Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12135

Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBWI76_RS12110 and BWI76_RS12115 overlap by 4 nucleotidesBWI76_RS12115 and BWI76_RS12120 overlap by 8 nucleotidesBWI76_RS12120 and BWI76_RS12125 are separated by 0 nucleotidesBWI76_RS12125 and BWI76_RS12130 overlap by 1 nucleotidesBWI76_RS12130 and BWI76_RS12135 overlap by 4 nucleotidesBWI76_RS12135 and BWI76_RS12140 are separated by 248 nucleotidesBWI76_RS12140 and BWI76_RS12145 overlap by 1 nucleotides BWI76_RS12110: BWI76_RS12110 - hypothetical protein, at 2,542,145 to 2,542,930 _RS12110 BWI76_RS12115: BWI76_RS12115 - hypothetical protein, at 2,542,927 to 2,543,130 _RS12115 BWI76_RS12120: BWI76_RS12120 - hypothetical protein, at 2,543,123 to 2,543,308 _RS12120 BWI76_RS12125: BWI76_RS12125 - hypothetical protein, at 2,543,309 to 2,543,521 _RS12125 BWI76_RS12130: BWI76_RS12130 - hypothetical protein, at 2,543,521 to 2,543,778 _RS12130 BWI76_RS12135: BWI76_RS12135 - C-5 cytosine-specific DNA methylase, at 2,543,775 to 2,545,757 _RS12135 BWI76_RS12140: BWI76_RS12140 - hypothetical protein, at 2,546,006 to 2,546,659 _RS12140 BWI76_RS12145: BWI76_RS12145 - hypothetical protein, at 2,546,659 to 2,547,765 _RS12145 Position (kb) 2543 2544 2545 2546Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2542.866 kb on + strandat 2543.010 kb on - strand, within BWI76_RS12115at 2543.010 kb on - strand, within BWI76_RS12115at 2543.015 kb on - strand, within BWI76_RS12115at 2543.015 kb on - strand, within BWI76_RS12115at 2543.107 kb on - strand, within BWI76_RS12115at 2543.108 kb on - strand, within BWI76_RS12115at 2543.311 kb on + strandat 2543.311 kb on + strandat 2543.311 kb on + strandat 2543.456 kb on + strand, within BWI76_RS12125at 2543.490 kb on - strand, within BWI76_RS12125at 2543.913 kb on - strandat 2543.927 kb on + strandat 2544.089 kb on - strand, within BWI76_RS12135at 2544.253 kb on + strandat 2544.529 kb on + strand, within BWI76_RS12135at 2544.604 kb on + strand, within BWI76_RS12135at 2544.614 kb on - strand, within BWI76_RS12135at 2544.890 kb on - strand, within BWI76_RS12135at 2544.890 kb on - strand, within BWI76_RS12135at 2545.115 kb on + strand, within BWI76_RS12135at 2545.115 kb on + strand, within BWI76_RS12135at 2545.327 kb on + strand, within BWI76_RS12135at 2545.454 kb on - strand, within BWI76_RS12135at 2545.507 kb on - strand, within BWI76_RS12135at 2545.579 kb on + strandat 2545.580 kb on + strandat 2545.580 kb on + strandat 2545.588 kb on - strandat 2545.590 kb on + strandat 2545.590 kb on + strandat 2545.598 kb on - strandat 2545.603 kb on - strandat 2545.603 kb on - strandat 2545.603 kb on - strandat 2545.625 kb on + strandat 2545.625 kb on + strandat 2545.635 kb on + strandat 2545.672 kb on + strandat 2545.672 kb on + strandat 2545.672 kb on + strandat 2545.672 kb on + strandat 2545.680 kb on - strandat 2545.681 kb on - strandat 2545.681 kb on - strandat 2545.704 kb on + strandat 2545.709 kb on + strandat 2545.709 kb on + strandat 2545.714 kb on + strandat 2545.714 kb on + strandat 2545.714 kb on + strandat 2545.714 kb on + strandat 2545.714 kb on + strandat 2545.717 kb on - strandat 2545.722 kb on - strandat 2545.728 kb on + strandat 2545.733 kb on + strandat 2545.738 kb on + strandat 2545.738 kb on + strandat 2545.741 kb on - strandat 2545.741 kb on - strandat 2545.746 kb on - strandat 2545.786 kb on + strandat 2545.811 kb on + strandat 2545.814 kb on - strandat 2545.814 kb on - strandat 2545.819 kb on - strandat 2545.824 kb on + strandat 2545.832 kb on - strandat 2545.840 kb on - strandat 2545.886 kb on + strandat 2545.886 kb on + strandat 2545.894 kb on - strandat 2545.894 kb on - strandat 2545.894 kb on - strandat 2545.894 kb on - strandat 2545.894 kb on - strandat 2545.894 kb on - strandat 2545.894 kb on - strandat 2545.925 kb on - strandat 2545.930 kb on - strandat 2545.930 kb on - strandat 2545.930 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
remove
2,542,866 + +0.4
2,543,010 - BWI76_RS12115 0.41 -0.7
2,543,010 - BWI76_RS12115 0.41 -1.0
2,543,015 - BWI76_RS12115 0.43 -0.3
2,543,015 - BWI76_RS12115 0.43 -0.1
2,543,107 - BWI76_RS12115 0.88 -0.7
2,543,108 - BWI76_RS12115 0.89 -1.2
2,543,311 + -0.9
2,543,311 + -0.5
2,543,311 + -0.3
2,543,456 + BWI76_RS12125 0.69 -0.5
2,543,490 - BWI76_RS12125 0.85 -1.5
2,543,913 - -0.3
2,543,927 + -0.3
2,544,089 - BWI76_RS12135 0.16 -2.4
2,544,253 + -0.5
2,544,529 + BWI76_RS12135 0.38 -0.8
2,544,604 + BWI76_RS12135 0.42 -1.4
2,544,614 - BWI76_RS12135 0.42 -0.3
2,544,890 - BWI76_RS12135 0.56 -0.5
2,544,890 - BWI76_RS12135 0.56 -0.3
2,545,115 + BWI76_RS12135 0.68 +0.2
2,545,115 + BWI76_RS12135 0.68 -0.1
2,545,327 + BWI76_RS12135 0.78 -0.6
2,545,454 - BWI76_RS12135 0.85 -1.9
2,545,507 - BWI76_RS12135 0.87 -3.4
2,545,579 + -1.4
2,545,580 + -0.1
2,545,580 + -0.2
2,545,588 - -2.5
2,545,590 + +0.3
2,545,590 + -1.1
2,545,598 - +0.0
2,545,603 - -1.2
2,545,603 - -1.0
2,545,603 - -1.1
2,545,625 + -0.4
2,545,625 + -0.4
2,545,635 + -0.2
2,545,672 + -1.0
2,545,672 + +0.1
2,545,672 + -1.2
2,545,672 + -0.7
2,545,680 - -1.1
2,545,681 - -1.5
2,545,681 - -0.1
2,545,704 + -1.4
2,545,709 + -1.0
2,545,709 + +0.4
2,545,714 + +0.1
2,545,714 + -2.7
2,545,714 + +0.9
2,545,714 + -0.3
2,545,714 + -1.8
2,545,717 - -1.9
2,545,722 - +0.5
2,545,728 + -0.6
2,545,733 + -2.5
2,545,738 + -1.5
2,545,738 + +0.1
2,545,741 - -2.2
2,545,741 - -1.7
2,545,746 - +0.5
2,545,786 + -1.2
2,545,811 + -1.4
2,545,814 - -0.9
2,545,814 - -0.6
2,545,819 - -0.3
2,545,824 + -0.4
2,545,832 - -0.3
2,545,840 - -0.4
2,545,886 + -0.9
2,545,886 + -2.1
2,545,894 - -0.2
2,545,894 - -0.3
2,545,894 - -0.5
2,545,894 - -0.1
2,545,894 - -2.0
2,545,894 - +1.8
2,545,894 - -0.6
2,545,925 - +0.1
2,545,930 - -0.8
2,545,930 - -0.9
2,545,930 - +0.2

Or see this region's nucleotide sequence