Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10855 and BWI76_RS10860 overlap by 4 nucleotides BWI76_RS10860 and BWI76_RS10865 are separated by 288 nucleotides BWI76_RS10865 and BWI76_RS10870 are separated by 145 nucleotides
BWI76_RS10855: BWI76_RS10855 - RND family efflux transporter MFP subunit, at 2,293,384 to 2,294,532
_RS10855
BWI76_RS10860: BWI76_RS10860 - ACR family transporter, at 2,294,529 to 2,297,603
_RS10860
BWI76_RS10865: BWI76_RS10865 - tRNA-Ser, at 2,297,892 to 2,297,979
_RS10865
BWI76_RS10870: BWI76_RS10870 - nicotinamidase, at 2,298,125 to 2,298,802
_RS10870
Position (kb)
2294
2295
2296
2297
2298 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2293.656 kb on + strand, within BWI76_RS10855 at 2294.037 kb on - strand, within BWI76_RS10855 at 2294.638 kb on + strand at 2294.638 kb on + strand at 2294.644 kb on - strand at 2295.155 kb on - strand at 2295.263 kb on - strand, within BWI76_RS10860 at 2295.496 kb on + strand, within BWI76_RS10860 at 2295.496 kb on + strand, within BWI76_RS10860 at 2295.496 kb on + strand, within BWI76_RS10860 at 2295.901 kb on + strand, within BWI76_RS10860 at 2296.007 kb on - strand, within BWI76_RS10860 at 2296.017 kb on - strand, within BWI76_RS10860 at 2296.201 kb on + strand, within BWI76_RS10860 at 2296.611 kb on + strand, within BWI76_RS10860 at 2296.735 kb on - strand, within BWI76_RS10860 at 2296.735 kb on - strand, within BWI76_RS10860 at 2296.901 kb on + strand, within BWI76_RS10860 at 2297.067 kb on - strand, within BWI76_RS10860 at 2297.198 kb on + strand, within BWI76_RS10860 at 2297.455 kb on + strand at 2297.629 kb on - strand at 2298.029 kb on - strand at 2298.309 kb on - strand, within BWI76_RS10870 at 2298.319 kb on - strand, within BWI76_RS10870
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,293,656 + BWI76_RS10855 0.24 -0.1 2,294,037 - BWI76_RS10855 0.57 -0.4 2,294,638 + +2.5 2,294,638 + -1.7 2,294,644 - -0.2 2,295,155 - +0.9 2,295,263 - BWI76_RS10860 0.24 +0.9 2,295,496 + BWI76_RS10860 0.31 -2.0 2,295,496 + BWI76_RS10860 0.31 -0.1 2,295,496 + BWI76_RS10860 0.31 +0.7 2,295,901 + BWI76_RS10860 0.45 +0.9 2,296,007 - BWI76_RS10860 0.48 -0.3 2,296,017 - BWI76_RS10860 0.48 +0.8 2,296,201 + BWI76_RS10860 0.54 +0.2 2,296,611 + BWI76_RS10860 0.68 +0.0 2,296,735 - BWI76_RS10860 0.72 +0.9 2,296,735 - BWI76_RS10860 0.72 +0.3 2,296,901 + BWI76_RS10860 0.77 -0.1 2,297,067 - BWI76_RS10860 0.83 -0.4 2,297,198 + BWI76_RS10860 0.87 -1.0 2,297,455 + +1.2 2,297,629 - -0.1 2,298,029 - -0.4 2,298,309 - BWI76_RS10870 0.27 +0.3 2,298,319 - BWI76_RS10870 0.29 -0.0
Or see this region's nucleotide sequence