Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04420 and BWI76_RS04425 are separated by 15 nucleotides BWI76_RS04425 and BWI76_RS04430 are separated by 160 nucleotides BWI76_RS04430 and BWI76_RS04435 are separated by 278 nucleotides
BWI76_RS04420: BWI76_RS04420 - oxaloacetate decarboxylase, at 912,522 to 914,288
_RS04420
BWI76_RS04425: BWI76_RS04425 - oxaloacetate decarboxylase subunit gamma, at 914,304 to 914,555
_RS04425
BWI76_RS04430: BWI76_RS04430 - citrate carrier protein, at 914,716 to 916,056
_RS04430
BWI76_RS04435: BWI76_RS04435 - [citrate (pro-3S)-lyase] ligase, at 916,335 to 917,363
_RS04435
Position (kb) 914
915
916
917 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 913.825 kb on - strand, within BWI76_RS04420 at 913.835 kb on - strand, within BWI76_RS04420 at 913.863 kb on - strand, within BWI76_RS04420 at 914.101 kb on + strand at 914.511 kb on + strand, within BWI76_RS04425 at 914.540 kb on - strand at 914.630 kb on - strand at 914.820 kb on - strand at 914.820 kb on - strand at 914.836 kb on - strand at 914.861 kb on + strand, within BWI76_RS04430 at 914.866 kb on - strand, within BWI76_RS04430 at 914.868 kb on + strand, within BWI76_RS04430 at 915.014 kb on - strand, within BWI76_RS04430 at 915.019 kb on - strand, within BWI76_RS04430 at 915.121 kb on + strand, within BWI76_RS04430 at 915.129 kb on - strand, within BWI76_RS04430 at 915.136 kb on + strand, within BWI76_RS04430 at 915.214 kb on - strand, within BWI76_RS04430 at 915.244 kb on + strand at 915.324 kb on + strand, within BWI76_RS04430 at 915.342 kb on - strand, within BWI76_RS04430 at 915.389 kb on + strand, within BWI76_RS04430 at 915.397 kb on - strand, within BWI76_RS04430 at 915.400 kb on + strand, within BWI76_RS04430 at 915.406 kb on + strand, within BWI76_RS04430 at 915.411 kb on + strand at 915.423 kb on + strand, within BWI76_RS04430 at 915.423 kb on + strand, within BWI76_RS04430 at 915.425 kb on + strand, within BWI76_RS04430 at 915.430 kb on + strand, within BWI76_RS04430 at 915.431 kb on - strand, within BWI76_RS04430 at 915.431 kb on - strand, within BWI76_RS04430 at 915.438 kb on - strand, within BWI76_RS04430 at 915.478 kb on + strand, within BWI76_RS04430 at 915.497 kb on - strand, within BWI76_RS04430 at 915.497 kb on - strand, within BWI76_RS04430 at 915.531 kb on - strand, within BWI76_RS04430 at 915.532 kb on - strand, within BWI76_RS04430 at 915.532 kb on - strand, within BWI76_RS04430 at 915.534 kb on - strand, within BWI76_RS04430 at 915.557 kb on - strand, within BWI76_RS04430 at 915.571 kb on + strand, within BWI76_RS04430 at 915.576 kb on - strand, within BWI76_RS04430 at 915.579 kb on - strand, within BWI76_RS04430 at 915.589 kb on - strand, within BWI76_RS04430 at 915.601 kb on - strand, within BWI76_RS04430 at 915.601 kb on - strand, within BWI76_RS04430 at 915.621 kb on + strand, within BWI76_RS04430 at 915.629 kb on - strand, within BWI76_RS04430 at 915.629 kb on - strand, within BWI76_RS04430 at 915.629 kb on - strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.658 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.680 kb on + strand, within BWI76_RS04430 at 915.698 kb on + strand, within BWI76_RS04430 at 915.741 kb on + strand, within BWI76_RS04430 at 915.741 kb on + strand, within BWI76_RS04430 at 915.756 kb on + strand, within BWI76_RS04430 at 915.799 kb on + strand, within BWI76_RS04430 at 915.818 kb on + strand, within BWI76_RS04430 at 915.892 kb on - strand, within BWI76_RS04430 at 915.895 kb on + strand, within BWI76_RS04430 at 915.915 kb on + strand, within BWI76_RS04430 at 915.923 kb on - strand at 915.954 kb on + strand at 915.954 kb on + strand at 915.957 kb on - strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.962 kb on - strand at 915.969 kb on - strand at 915.969 kb on - strand at 915.969 kb on - strand at 915.970 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.044 kb on - strand at 916.068 kb on - strand at 916.238 kb on + strand at 916.294 kb on + strand at 916.294 kb on + strand at 916.303 kb on - strand at 916.389 kb on + strand at 916.390 kb on + strand at 916.390 kb on - strand at 916.397 kb on + strand at 916.398 kb on - strand at 916.418 kb on + strand at 916.418 kb on + strand at 916.426 kb on - strand at 916.438 kb on + strand, within BWI76_RS04435 at 916.461 kb on + strand, within BWI76_RS04435 at 916.503 kb on + strand, within BWI76_RS04435 at 916.503 kb on + strand, within BWI76_RS04435 at 916.526 kb on - strand, within BWI76_RS04435 at 916.552 kb on + strand, within BWI76_RS04435 at 916.552 kb on + strand, within BWI76_RS04435 at 916.560 kb on - strand, within BWI76_RS04435 at 916.560 kb on - strand, within BWI76_RS04435 at 916.565 kb on + strand, within BWI76_RS04435 at 916.571 kb on + strand, within BWI76_RS04435 at 916.585 kb on + strand, within BWI76_RS04435 at 916.830 kb on - strand, within BWI76_RS04435
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 913,825 - BWI76_RS04420 0.74 -0.7 913,835 - BWI76_RS04420 0.74 +0.0 913,863 - BWI76_RS04420 0.76 +0.2 914,101 + -2.1 914,511 + BWI76_RS04425 0.82 +0.0 914,540 - -1.1 914,630 - +0.6 914,820 - +0.9 914,820 - -0.6 914,836 - -0.3 914,861 + BWI76_RS04430 0.11 -0.6 914,866 - BWI76_RS04430 0.11 -1.4 914,868 + BWI76_RS04430 0.11 -0.1 915,014 - BWI76_RS04430 0.22 +0.3 915,019 - BWI76_RS04430 0.23 -0.3 915,121 + BWI76_RS04430 0.30 +0.6 915,129 - BWI76_RS04430 0.31 -0.1 915,136 + BWI76_RS04430 0.31 -0.2 915,214 - BWI76_RS04430 0.37 -0.7 915,244 + -0.1 915,324 + BWI76_RS04430 0.45 -0.9 915,342 - BWI76_RS04430 0.47 -0.8 915,389 + BWI76_RS04430 0.50 -0.4 915,397 - BWI76_RS04430 0.51 -1.3 915,400 + BWI76_RS04430 0.51 -2.5 915,406 + BWI76_RS04430 0.51 -1.3 915,411 + -0.9 915,423 + BWI76_RS04430 0.53 -0.2 915,423 + BWI76_RS04430 0.53 -0.3 915,425 + BWI76_RS04430 0.53 +1.0 915,430 + BWI76_RS04430 0.53 -0.7 915,431 - BWI76_RS04430 0.53 -1.2 915,431 - BWI76_RS04430 0.53 -1.1 915,438 - BWI76_RS04430 0.54 +0.3 915,478 + BWI76_RS04430 0.57 +0.1 915,497 - BWI76_RS04430 0.58 -1.2 915,497 - BWI76_RS04430 0.58 -0.7 915,531 - BWI76_RS04430 0.61 -0.1 915,532 - BWI76_RS04430 0.61 -0.7 915,532 - BWI76_RS04430 0.61 -0.1 915,534 - BWI76_RS04430 0.61 -1.2 915,557 - BWI76_RS04430 0.63 +0.7 915,571 + BWI76_RS04430 0.64 -1.0 915,576 - BWI76_RS04430 0.64 -1.0 915,579 - BWI76_RS04430 0.64 +1.7 915,589 - BWI76_RS04430 0.65 +0.9 915,601 - BWI76_RS04430 0.66 +0.1 915,601 - BWI76_RS04430 0.66 -0.6 915,621 + BWI76_RS04430 0.67 +0.7 915,629 - BWI76_RS04430 0.68 +0.7 915,629 - BWI76_RS04430 0.68 -0.1 915,629 - BWI76_RS04430 0.68 -0.7 915,651 + BWI76_RS04430 0.70 -1.1 915,651 + BWI76_RS04430 0.70 +0.7 915,651 + BWI76_RS04430 0.70 +0.6 915,651 + BWI76_RS04430 0.70 +0.4 915,651 + BWI76_RS04430 0.70 -1.1 915,651 + BWI76_RS04430 0.70 -2.4 915,651 + BWI76_RS04430 0.70 +1.4 915,651 + BWI76_RS04430 0.70 -0.7 915,651 + -1.9 915,651 + BWI76_RS04430 0.70 +0.4 915,651 + BWI76_RS04430 0.70 -1.7 915,651 + BWI76_RS04430 0.70 +0.5 915,651 + BWI76_RS04430 0.70 -0.5 915,651 + BWI76_RS04430 0.70 +3.2 915,658 - BWI76_RS04430 0.70 -1.3 915,659 - BWI76_RS04430 0.70 +0.2 915,659 - BWI76_RS04430 0.70 +0.6 915,659 - BWI76_RS04430 0.70 +0.9 915,659 - BWI76_RS04430 0.70 -0.1 915,659 - BWI76_RS04430 0.70 -1.3 915,680 + BWI76_RS04430 0.72 -1.6 915,698 + BWI76_RS04430 0.73 +0.9 915,741 + BWI76_RS04430 0.76 -1.3 915,741 + BWI76_RS04430 0.76 -1.6 915,756 + BWI76_RS04430 0.78 -1.1 915,799 + BWI76_RS04430 0.81 +0.1 915,818 + BWI76_RS04430 0.82 -0.8 915,892 - BWI76_RS04430 0.88 +0.9 915,895 + BWI76_RS04430 0.88 -0.1 915,915 + BWI76_RS04430 0.89 -0.4 915,923 - -0.5 915,954 + -0.8 915,954 + -0.1 915,957 - -1.9 915,961 + -0.5 915,961 + -0.7 915,961 + +0.9 915,961 + +0.9 915,961 + +0.9 915,962 - +1.1 915,969 - -1.7 915,969 - -1.1 915,969 - +0.6 915,970 - -1.1 916,039 - -1.9 916,039 - -2.7 916,039 - +0.5 916,039 - -2.3 916,039 - -1.7 916,039 - -0.1 916,039 - -0.4 916,044 - -0.6 916,068 - +0.9 916,238 + -1.1 916,294 + +2.9 916,294 + -1.1 916,303 - +0.4 916,389 + +0.0 916,390 + +1.0 916,390 - -1.0 916,397 + +0.6 916,398 - +1.2 916,418 + -0.7 916,418 + -0.9 916,426 - -2.5 916,438 + BWI76_RS04435 0.10 +0.2 916,461 + BWI76_RS04435 0.12 -0.7 916,503 + BWI76_RS04435 0.16 -0.6 916,503 + BWI76_RS04435 0.16 +0.3 916,526 - BWI76_RS04435 0.19 -0.1 916,552 + BWI76_RS04435 0.21 +0.9 916,552 + BWI76_RS04435 0.21 -0.3 916,560 - BWI76_RS04435 0.22 -0.7 916,560 - BWI76_RS04435 0.22 -0.5 916,565 + BWI76_RS04435 0.22 +1.4 916,571 + BWI76_RS04435 0.23 +0.3 916,585 + BWI76_RS04435 0.24 +0.4 916,830 - BWI76_RS04435 0.48 +0.2
Or see this region's nucleotide sequence