Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21705 and BWI76_RS21710 are separated by 171 nucleotides BWI76_RS21710 and BWI76_RS21715 overlap by 10 nucleotides BWI76_RS21715 and BWI76_RS21720 are separated by 1 nucleotides BWI76_RS21720 and BWI76_RS21725 overlap by 52 nucleotides BWI76_RS21725 and BWI76_RS21730 are separated by 92 nucleotides BWI76_RS21730 and BWI76_RS21735 are separated by 14 nucleotides
BWI76_RS21705: BWI76_RS21705 - MFS transporter, at 4,539,495 to 4,540,685
_RS21705
BWI76_RS21710: BWI76_RS21710 - nitroreductase, at 4,540,857 to 4,541,231
_RS21710
BWI76_RS21715: BWI76_RS21715 - transcriptional regulator, at 4,541,222 to 4,541,401
_RS21715
BWI76_RS21720: BWI76_RS21720 - hypothetical protein, at 4,541,403 to 4,541,645
_RS21720
BWI76_RS21725: BWI76_RS21725 - flavin reductase, at 4,541,594 to 4,542,166
_RS21725
BWI76_RS21730: BWI76_RS21730 - hypothetical protein, at 4,542,259 to 4,542,774
_RS21730
BWI76_RS21735: BWI76_RS21735 - aldehyde oxidase, at 4,542,789 to 4,543,754
_RS21735
Position (kb) 4541
4542
4543 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4540.689 kb on + strand at 4540.697 kb on - strand at 4540.697 kb on - strand at 4540.707 kb on - strand at 4540.724 kb on - strand at 4540.751 kb on - strand at 4540.772 kb on + strand at 4540.772 kb on + strand at 4540.772 kb on + strand at 4540.777 kb on + strand at 4540.782 kb on + strand at 4540.815 kb on + strand at 4540.855 kb on + strand at 4540.863 kb on - strand at 4540.863 kb on - strand at 4540.863 kb on - strand at 4540.868 kb on - strand at 4540.904 kb on - strand, within BWI76_RS21710 at 4540.925 kb on + strand, within BWI76_RS21710 at 4540.926 kb on - strand, within BWI76_RS21710 at 4541.051 kb on + strand, within BWI76_RS21710 at 4541.062 kb on + strand, within BWI76_RS21710 at 4541.083 kb on + strand, within BWI76_RS21710 at 4541.159 kb on + strand, within BWI76_RS21710 at 4541.249 kb on - strand, within BWI76_RS21715 at 4541.658 kb on + strand, within BWI76_RS21725 at 4541.673 kb on - strand, within BWI76_RS21725 at 4541.797 kb on + strand, within BWI76_RS21725 at 4541.818 kb on + strand, within BWI76_RS21725 at 4541.823 kb on + strand, within BWI76_RS21725 at 4541.823 kb on + strand, within BWI76_RS21725 at 4541.836 kb on - strand, within BWI76_RS21725 at 4541.836 kb on - strand, within BWI76_RS21725 at 4541.887 kb on + strand, within BWI76_RS21725 at 4541.914 kb on - strand, within BWI76_RS21725 at 4541.917 kb on - strand, within BWI76_RS21725 at 4541.917 kb on - strand, within BWI76_RS21725 at 4541.922 kb on - strand, within BWI76_RS21725 at 4541.935 kb on + strand, within BWI76_RS21725 at 4541.953 kb on - strand, within BWI76_RS21725 at 4541.996 kb on + strand, within BWI76_RS21725 at 4541.997 kb on + strand, within BWI76_RS21725 at 4541.997 kb on + strand, within BWI76_RS21725 at 4541.997 kb on + strand, within BWI76_RS21725 at 4542.005 kb on - strand, within BWI76_RS21725 at 4542.005 kb on - strand, within BWI76_RS21725 at 4542.005 kb on - strand, within BWI76_RS21725 at 4542.021 kb on + strand, within BWI76_RS21725 at 4542.022 kb on - strand, within BWI76_RS21725 at 4542.028 kb on - strand, within BWI76_RS21725 at 4542.040 kb on + strand, within BWI76_RS21725 at 4542.054 kb on - strand, within BWI76_RS21725 at 4542.060 kb on - strand, within BWI76_RS21725 at 4542.064 kb on + strand, within BWI76_RS21725 at 4542.088 kb on - strand, within BWI76_RS21725 at 4542.117 kb on + strand at 4542.135 kb on + strand at 4542.153 kb on - strand at 4542.153 kb on - strand at 4542.213 kb on + strand at 4542.235 kb on + strand at 4542.262 kb on + strand at 4542.262 kb on + strand at 4542.270 kb on - strand at 4542.281 kb on + strand at 4542.350 kb on - strand, within BWI76_RS21730 at 4542.438 kb on - strand, within BWI76_RS21730 at 4542.441 kb on + strand, within BWI76_RS21730 at 4542.449 kb on - strand, within BWI76_RS21730 at 4542.566 kb on + strand, within BWI76_RS21730 at 4542.566 kb on + strand, within BWI76_RS21730 at 4542.605 kb on + strand, within BWI76_RS21730 at 4542.653 kb on + strand, within BWI76_RS21730 at 4542.660 kb on + strand, within BWI76_RS21730 at 4542.668 kb on - strand, within BWI76_RS21730 at 4542.678 kb on + strand, within BWI76_RS21730 at 4542.678 kb on + strand, within BWI76_RS21730 at 4542.678 kb on + strand, within BWI76_RS21730 at 4542.686 kb on - strand, within BWI76_RS21730 at 4542.686 kb on - strand, within BWI76_RS21730 at 4542.686 kb on - strand, within BWI76_RS21730 at 4542.686 kb on - strand, within BWI76_RS21730 at 4542.691 kb on + strand, within BWI76_RS21730 at 4542.696 kb on + strand, within BWI76_RS21730 at 4542.701 kb on - strand, within BWI76_RS21730 at 4542.712 kb on - strand, within BWI76_RS21730 at 4542.712 kb on - strand, within BWI76_RS21730 at 4542.714 kb on + strand, within BWI76_RS21730 at 4542.722 kb on - strand, within BWI76_RS21730 at 4542.723 kb on - strand at 4542.725 kb on + strand at 4542.727 kb on - strand at 4542.727 kb on - strand at 4542.733 kb on - strand at 4542.735 kb on + strand at 4542.740 kb on + strand at 4542.819 kb on + strand at 4542.819 kb on + strand at 4542.819 kb on + strand at 4542.827 kb on - strand at 4542.827 kb on - strand at 4542.827 kb on - strand at 4542.827 kb on - strand at 4542.827 kb on - strand at 4542.827 kb on - strand at 4542.827 kb on - strand at 4542.866 kb on - strand at 4542.902 kb on + strand, within BWI76_RS21735 at 4542.910 kb on - strand, within BWI76_RS21735 at 4542.928 kb on + strand, within BWI76_RS21735 at 4542.936 kb on - strand, within BWI76_RS21735 at 4542.936 kb on - strand, within BWI76_RS21735 at 4542.936 kb on - strand, within BWI76_RS21735 at 4543.006 kb on + strand, within BWI76_RS21735 at 4543.089 kb on + strand, within BWI76_RS21735 at 4543.091 kb on - strand, within BWI76_RS21735 at 4543.099 kb on + strand, within BWI76_RS21735 at 4543.122 kb on - strand, within BWI76_RS21735
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,540,689 + -2.2 4,540,697 - -0.8 4,540,697 - +1.1 4,540,707 - +1.6 4,540,724 - -0.9 4,540,751 - +0.1 4,540,772 + +1.2 4,540,772 + -1.7 4,540,772 + -0.5 4,540,777 + -0.2 4,540,782 + +0.5 4,540,815 + +0.8 4,540,855 + +1.1 4,540,863 - +0.9 4,540,863 - -0.3 4,540,863 - -1.4 4,540,868 - +0.1 4,540,904 - BWI76_RS21710 0.13 +1.6 4,540,925 + BWI76_RS21710 0.18 +0.1 4,540,926 - BWI76_RS21710 0.18 -1.9 4,541,051 + BWI76_RS21710 0.52 -0.4 4,541,062 + BWI76_RS21710 0.55 +0.1 4,541,083 + BWI76_RS21710 0.60 -0.0 4,541,159 + BWI76_RS21710 0.81 -1.7 4,541,249 - BWI76_RS21715 0.15 +0.6 4,541,658 + BWI76_RS21725 0.11 -2.9 4,541,673 - BWI76_RS21725 0.14 -0.9 4,541,797 + BWI76_RS21725 0.35 -0.4 4,541,818 + BWI76_RS21725 0.39 +0.3 4,541,823 + BWI76_RS21725 0.40 -0.7 4,541,823 + BWI76_RS21725 0.40 -3.3 4,541,836 - BWI76_RS21725 0.42 +0.6 4,541,836 - BWI76_RS21725 0.42 -1.3 4,541,887 + BWI76_RS21725 0.51 -1.6 4,541,914 - BWI76_RS21725 0.56 -0.1 4,541,917 - BWI76_RS21725 0.56 -0.5 4,541,917 - BWI76_RS21725 0.56 +0.6 4,541,922 - BWI76_RS21725 0.57 -2.1 4,541,935 + BWI76_RS21725 0.60 +1.0 4,541,953 - BWI76_RS21725 0.63 -0.7 4,541,996 + BWI76_RS21725 0.70 -0.7 4,541,997 + BWI76_RS21725 0.70 -2.3 4,541,997 + BWI76_RS21725 0.70 -0.3 4,541,997 + BWI76_RS21725 0.70 -2.4 4,542,005 - BWI76_RS21725 0.72 -1.6 4,542,005 - BWI76_RS21725 0.72 -0.9 4,542,005 - BWI76_RS21725 0.72 -1.0 4,542,021 + BWI76_RS21725 0.75 -1.1 4,542,022 - BWI76_RS21725 0.75 +0.8 4,542,028 - BWI76_RS21725 0.76 -1.0 4,542,040 + BWI76_RS21725 0.78 -1.7 4,542,054 - BWI76_RS21725 0.80 -0.2 4,542,060 - BWI76_RS21725 0.81 +0.4 4,542,064 + BWI76_RS21725 0.82 +0.1 4,542,088 - BWI76_RS21725 0.86 +1.0 4,542,117 + -0.7 4,542,135 + +1.6 4,542,153 - +0.8 4,542,153 - -0.1 4,542,213 + +1.6 4,542,235 + +0.2 4,542,262 + -0.8 4,542,262 + -0.7 4,542,270 - -1.0 4,542,281 + +1.3 4,542,350 - BWI76_RS21730 0.18 +0.0 4,542,438 - BWI76_RS21730 0.35 +1.3 4,542,441 + BWI76_RS21730 0.35 -0.6 4,542,449 - BWI76_RS21730 0.37 -0.3 4,542,566 + BWI76_RS21730 0.59 +0.9 4,542,566 + BWI76_RS21730 0.59 +0.2 4,542,605 + BWI76_RS21730 0.67 +0.5 4,542,653 + BWI76_RS21730 0.76 -0.9 4,542,660 + BWI76_RS21730 0.78 -1.5 4,542,668 - BWI76_RS21730 0.79 -0.0 4,542,678 + BWI76_RS21730 0.81 -0.7 4,542,678 + BWI76_RS21730 0.81 -0.1 4,542,678 + BWI76_RS21730 0.81 -0.4 4,542,686 - BWI76_RS21730 0.83 -0.4 4,542,686 - BWI76_RS21730 0.83 -0.1 4,542,686 - BWI76_RS21730 0.83 +2.6 4,542,686 - BWI76_RS21730 0.83 +0.9 4,542,691 + BWI76_RS21730 0.84 -3.5 4,542,696 + BWI76_RS21730 0.85 -1.1 4,542,701 - BWI76_RS21730 0.86 +0.4 4,542,712 - BWI76_RS21730 0.88 +0.4 4,542,712 - BWI76_RS21730 0.88 -0.2 4,542,714 + BWI76_RS21730 0.88 -0.1 4,542,722 - BWI76_RS21730 0.90 +0.2 4,542,723 - +0.4 4,542,725 + +1.1 4,542,727 - -0.8 4,542,727 - +0.3 4,542,733 - -1.2 4,542,735 + -2.0 4,542,740 + -0.3 4,542,819 + -1.3 4,542,819 + +1.4 4,542,819 + -0.9 4,542,827 - -0.2 4,542,827 - +0.6 4,542,827 - -0.1 4,542,827 - -0.5 4,542,827 - +0.6 4,542,827 - +0.6 4,542,827 - -1.9 4,542,866 - +0.8 4,542,902 + BWI76_RS21735 0.12 +1.6 4,542,910 - BWI76_RS21735 0.13 -0.8 4,542,928 + BWI76_RS21735 0.14 -0.1 4,542,936 - BWI76_RS21735 0.15 -0.1 4,542,936 - BWI76_RS21735 0.15 +0.3 4,542,936 - BWI76_RS21735 0.15 -0.5 4,543,006 + BWI76_RS21735 0.22 -0.2 4,543,089 + BWI76_RS21735 0.31 -0.3 4,543,091 - BWI76_RS21735 0.31 -1.1 4,543,099 + BWI76_RS21735 0.32 -2.2 4,543,122 - BWI76_RS21735 0.34 +0.4
Or see this region's nucleotide sequence