Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20650 and BWI76_RS20655 are separated by 42 nucleotides BWI76_RS20655 and BWI76_RS20660 are separated by 42 nucleotides BWI76_RS20660 and BWI76_RS20665 overlap by 4 nucleotides BWI76_RS20665 and BWI76_RS20670 are separated by 82 nucleotides
BWI76_RS20650: BWI76_RS20650 - phosphoenolpyruvate--protein phosphotransferase, at 4,320,863 to 4,322,590
_RS20650
BWI76_RS20655: BWI76_RS20655 - glucose-specific phosphotransferase enzyme IIA component, at 4,322,633 to 4,323,142
_RS20655
BWI76_RS20660: BWI76_RS20660 - hypothetical protein, at 4,323,185 to 4,323,424
_RS20660
BWI76_RS20665: BWI76_RS20665 - bifunctional pyridoxal kinase/hydroxymethylpyrimidine kinase, at 4,323,421 to 4,324,287
_RS20665
BWI76_RS20670: BWI76_RS20670 - transcriptional regulator PtsJ, at 4,324,370 to 4,325,668
_RS20670
Position (kb)
4323
4324
4325 Strain fitness (log2 ratio)
-2
-1
0
1 at 4322.527 kb on + strand at 4323.795 kb on + strand, within BWI76_RS20665 at 4323.959 kb on + strand, within BWI76_RS20665 at 4323.967 kb on - strand, within BWI76_RS20665 at 4324.019 kb on + strand, within BWI76_RS20665 at 4324.114 kb on - strand, within BWI76_RS20665 at 4324.152 kb on + strand, within BWI76_RS20665 at 4324.189 kb on + strand, within BWI76_RS20665 at 4324.220 kb on + strand at 4324.515 kb on + strand, within BWI76_RS20670 at 4324.533 kb on + strand, within BWI76_RS20670 at 4325.054 kb on - strand, within BWI76_RS20670 at 4325.140 kb on + strand, within BWI76_RS20670
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,322,527 + -0.1 4,323,795 + BWI76_RS20665 0.43 -0.7 4,323,959 + BWI76_RS20665 0.62 -0.6 4,323,967 - BWI76_RS20665 0.63 +1.5 4,324,019 + BWI76_RS20665 0.69 -1.1 4,324,114 - BWI76_RS20665 0.80 -0.4 4,324,152 + BWI76_RS20665 0.84 -2.4 4,324,189 + BWI76_RS20665 0.89 -1.6 4,324,220 + -0.0 4,324,515 + BWI76_RS20670 0.11 +0.4 4,324,533 + BWI76_RS20670 0.13 -0.1 4,325,054 - BWI76_RS20670 0.53 -0.3 4,325,140 + BWI76_RS20670 0.59 -1.0
Or see this region's nucleotide sequence