Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16385 and BWI76_RS16390 are separated by 596 nucleotides BWI76_RS16390 and BWI76_RS16395 are separated by 108 nucleotides BWI76_RS16395 and BWI76_RS16400 are separated by 47 nucleotides
BWI76_RS16385: BWI76_RS16385 - endonuclease III, at 3,399,202 to 3,399,837
_RS16385
BWI76_RS16390: BWI76_RS16390 - dipeptide/tripeptide permease A, at 3,400,434 to 3,401,939
_RS16390
BWI76_RS16395: BWI76_RS16395 - glutathione S-transferase, at 3,402,048 to 3,402,653
_RS16395
BWI76_RS16400: BWI76_RS16400 - pyridoxal kinase, at 3,402,701 to 3,403,561
_RS16400
Position (kb)
3400
3401
3402 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3399.711 kb on + strand, within BWI76_RS16385 at 3400.267 kb on - strand at 3400.308 kb on - strand at 3400.448 kb on + strand at 3400.488 kb on - strand at 3400.817 kb on + strand, within BWI76_RS16390 at 3400.817 kb on + strand, within BWI76_RS16390 at 3400.825 kb on - strand, within BWI76_RS16390 at 3401.195 kb on + strand, within BWI76_RS16390 at 3401.301 kb on + strand, within BWI76_RS16390 at 3401.391 kb on - strand, within BWI76_RS16390 at 3402.033 kb on + strand at 3402.111 kb on + strand, within BWI76_RS16395 at 3402.119 kb on - strand, within BWI76_RS16395 at 3402.407 kb on + strand, within BWI76_RS16395 at 3402.565 kb on - strand, within BWI76_RS16395 at 3402.727 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,399,711 + BWI76_RS16385 0.80 -1.8 3,400,267 - +0.3 3,400,308 - +1.7 3,400,448 + +1.8 3,400,488 - -1.1 3,400,817 + BWI76_RS16390 0.25 +1.7 3,400,817 + BWI76_RS16390 0.25 +0.8 3,400,825 - BWI76_RS16390 0.26 -0.3 3,401,195 + BWI76_RS16390 0.51 +0.6 3,401,301 + BWI76_RS16390 0.58 +1.3 3,401,391 - BWI76_RS16390 0.64 +0.9 3,402,033 + +0.2 3,402,111 + BWI76_RS16395 0.10 +0.8 3,402,119 - BWI76_RS16395 0.12 -0.3 3,402,407 + BWI76_RS16395 0.59 -0.4 3,402,565 - BWI76_RS16395 0.85 -1.2 3,402,727 + -0.4
Or see this region's nucleotide sequence