Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14205 and BWI76_RS14210 are separated by 181 nucleotides BWI76_RS14210 and BWI76_RS14215 are separated by 235 nucleotides BWI76_RS14215 and BWI76_RS14220 are separated by 21 nucleotides BWI76_RS14220 and BWI76_RS14225 are separated by 53 nucleotides
BWI76_RS14205: BWI76_RS14205 - aromatic acid/H+ symport family MFS transporter, at 2,973,111 to 2,974,480
_RS14205
BWI76_RS14210: BWI76_RS14210 - LysR family transcriptional regulator, at 2,974,662 to 2,975,624
_RS14210
BWI76_RS14215: BWI76_RS14215 - hypothetical protein, at 2,975,860 to 2,976,141
_RS14215
BWI76_RS14220: BWI76_RS14220 - hypothetical protein, at 2,976,163 to 2,976,504
_RS14220
BWI76_RS14225: BWI76_RS14225 - anti-ECF sigma factor ChrR, at 2,976,558 to 2,977,229
_RS14225
Position (kb)
2974
2975
2976 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2973.711 kb on + strand, within BWI76_RS14205 at 2973.745 kb on + strand, within BWI76_RS14205 at 2973.745 kb on + strand, within BWI76_RS14205 at 2973.745 kb on + strand, within BWI76_RS14205 at 2973.745 kb on + strand, within BWI76_RS14205 at 2973.753 kb on - strand, within BWI76_RS14205 at 2973.753 kb on - strand, within BWI76_RS14205 at 2973.753 kb on - strand, within BWI76_RS14205 at 2973.753 kb on - strand, within BWI76_RS14205 at 2973.866 kb on + strand, within BWI76_RS14205 at 2973.997 kb on - strand, within BWI76_RS14205 at 2974.002 kb on - strand, within BWI76_RS14205 at 2974.027 kb on + strand, within BWI76_RS14205 at 2974.056 kb on + strand, within BWI76_RS14205 at 2974.056 kb on + strand, within BWI76_RS14205 at 2974.197 kb on - strand, within BWI76_RS14205 at 2974.284 kb on - strand, within BWI76_RS14205 at 2974.349 kb on + strand at 2974.349 kb on + strand at 2974.349 kb on + strand at 2974.349 kb on + strand at 2974.349 kb on + strand at 2974.357 kb on - strand at 2974.357 kb on - strand at 2974.367 kb on - strand at 2974.371 kb on + strand at 2974.402 kb on + strand at 2974.405 kb on + strand at 2974.413 kb on - strand at 2974.442 kb on + strand at 2974.450 kb on + strand at 2974.476 kb on + strand at 2974.517 kb on - strand at 2974.521 kb on + strand at 2974.529 kb on - strand at 2974.550 kb on - strand at 2974.561 kb on + strand at 2974.642 kb on + strand at 2974.678 kb on + strand at 2974.730 kb on - strand at 2974.732 kb on + strand at 2974.740 kb on - strand at 2974.770 kb on + strand, within BWI76_RS14210 at 2974.774 kb on - strand, within BWI76_RS14210 at 2974.774 kb on - strand, within BWI76_RS14210 at 2974.778 kb on - strand, within BWI76_RS14210 at 2974.778 kb on - strand, within BWI76_RS14210 at 2974.792 kb on + strand, within BWI76_RS14210 at 2974.801 kb on - strand, within BWI76_RS14210 at 2974.801 kb on - strand, within BWI76_RS14210 at 2974.902 kb on + strand, within BWI76_RS14210 at 2974.902 kb on + strand, within BWI76_RS14210 at 2974.904 kb on - strand, within BWI76_RS14210 at 2974.957 kb on - strand, within BWI76_RS14210 at 2974.998 kb on + strand, within BWI76_RS14210 at 2975.194 kb on + strand, within BWI76_RS14210 at 2975.275 kb on - strand, within BWI76_RS14210 at 2975.500 kb on + strand, within BWI76_RS14210 at 2975.969 kb on + strand, within BWI76_RS14215 at 2975.970 kb on - strand, within BWI76_RS14215 at 2975.998 kb on - strand, within BWI76_RS14215 at 2976.025 kb on - strand, within BWI76_RS14215 at 2976.097 kb on + strand, within BWI76_RS14215 at 2976.097 kb on + strand, within BWI76_RS14215 at 2976.110 kb on + strand, within BWI76_RS14215 at 2976.123 kb on + strand at 2976.133 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,973,711 + BWI76_RS14205 0.44 +0.8 2,973,745 + BWI76_RS14205 0.46 -0.4 2,973,745 + BWI76_RS14205 0.46 +0.7 2,973,745 + BWI76_RS14205 0.46 -1.1 2,973,745 + BWI76_RS14205 0.46 -3.9 2,973,753 - BWI76_RS14205 0.47 +0.1 2,973,753 - BWI76_RS14205 0.47 +0.0 2,973,753 - BWI76_RS14205 0.47 -0.3 2,973,753 - BWI76_RS14205 0.47 +0.3 2,973,866 + BWI76_RS14205 0.55 +0.2 2,973,997 - BWI76_RS14205 0.65 -0.2 2,974,002 - BWI76_RS14205 0.65 +0.2 2,974,027 + BWI76_RS14205 0.67 -0.0 2,974,056 + BWI76_RS14205 0.69 -0.4 2,974,056 + BWI76_RS14205 0.69 -0.3 2,974,197 - BWI76_RS14205 0.79 +1.0 2,974,284 - BWI76_RS14205 0.86 -0.2 2,974,349 + -0.8 2,974,349 + -0.4 2,974,349 + -0.3 2,974,349 + +0.0 2,974,349 + -1.4 2,974,357 - +0.3 2,974,357 - +1.7 2,974,367 - -0.5 2,974,371 + +1.3 2,974,402 + -0.7 2,974,405 + +0.5 2,974,413 - +0.5 2,974,442 + -0.1 2,974,450 + -0.1 2,974,476 + -0.3 2,974,517 - -1.7 2,974,521 + -1.3 2,974,529 - -0.8 2,974,550 - +1.1 2,974,561 + +0.2 2,974,642 + +0.1 2,974,678 + -1.0 2,974,730 - -1.2 2,974,732 + -2.8 2,974,740 - -0.5 2,974,770 + BWI76_RS14210 0.11 -0.6 2,974,774 - BWI76_RS14210 0.12 -1.6 2,974,774 - BWI76_RS14210 0.12 -0.8 2,974,778 - BWI76_RS14210 0.12 -1.2 2,974,778 - BWI76_RS14210 0.12 +0.2 2,974,792 + BWI76_RS14210 0.13 -4.1 2,974,801 - BWI76_RS14210 0.14 -1.3 2,974,801 - BWI76_RS14210 0.14 -3.1 2,974,902 + BWI76_RS14210 0.25 +0.7 2,974,902 + BWI76_RS14210 0.25 -0.2 2,974,904 - BWI76_RS14210 0.25 -0.0 2,974,957 - BWI76_RS14210 0.31 -0.9 2,974,998 + BWI76_RS14210 0.35 +0.0 2,975,194 + BWI76_RS14210 0.55 +0.7 2,975,275 - BWI76_RS14210 0.64 +0.5 2,975,500 + BWI76_RS14210 0.87 -0.8 2,975,969 + BWI76_RS14215 0.39 +0.2 2,975,970 - BWI76_RS14215 0.39 +1.2 2,975,998 - BWI76_RS14215 0.49 +0.9 2,976,025 - BWI76_RS14215 0.59 +0.2 2,976,097 + BWI76_RS14215 0.84 +0.4 2,976,097 + BWI76_RS14215 0.84 +0.2 2,976,110 + BWI76_RS14215 0.89 -0.5 2,976,123 + +0.3 2,976,133 - -0.4
Or see this region's nucleotide sequence