Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13685 and BWI76_RS13690 are separated by 261 nucleotides BWI76_RS13690 and BWI76_RS13695 are separated by 12 nucleotides BWI76_RS13695 and BWI76_RS13700 are separated by 70 nucleotides
BWI76_RS13685: BWI76_RS13685 - IclR family transcriptional regulator, at 2,855,976 to 2,856,773
_RS13685
BWI76_RS13690: BWI76_RS13690 - muconate and chloromuconate cycloisomerase, at 2,857,035 to 2,858,153
_RS13690
BWI76_RS13695: BWI76_RS13695 - muconolactone delta-isomerase, at 2,858,166 to 2,858,456
_RS13695
BWI76_RS13700: BWI76_RS13700 - catechol 1,2-dioxygenase, at 2,858,527 to 2,859,453
_RS13700
Position (kb)
2857
2858
2859 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2856.585 kb on + strand, within BWI76_RS13685 at 2856.657 kb on - strand, within BWI76_RS13685 at 2857.252 kb on + strand at 2857.306 kb on + strand, within BWI76_RS13690 at 2857.314 kb on - strand, within BWI76_RS13690 at 2857.323 kb on + strand, within BWI76_RS13690 at 2857.379 kb on + strand, within BWI76_RS13690 at 2857.387 kb on - strand, within BWI76_RS13690 at 2857.387 kb on - strand, within BWI76_RS13690 at 2857.412 kb on + strand, within BWI76_RS13690 at 2857.457 kb on + strand, within BWI76_RS13690 at 2857.721 kb on + strand at 2857.729 kb on - strand, within BWI76_RS13690 at 2857.889 kb on + strand, within BWI76_RS13690 at 2857.978 kb on + strand, within BWI76_RS13690 at 2857.978 kb on + strand, within BWI76_RS13690 at 2857.978 kb on + strand, within BWI76_RS13690 at 2858.003 kb on - strand, within BWI76_RS13690 at 2858.022 kb on + strand, within BWI76_RS13690 at 2858.059 kb on + strand at 2858.067 kb on - strand at 2858.077 kb on - strand at 2858.082 kb on - strand at 2858.082 kb on - strand at 2858.100 kb on - strand at 2858.104 kb on + strand at 2858.104 kb on + strand at 2858.104 kb on + strand at 2858.104 kb on + strand at 2858.104 kb on + strand at 2858.104 kb on + strand at 2858.112 kb on - strand at 2858.112 kb on - strand at 2858.114 kb on + strand at 2858.115 kb on + strand at 2858.117 kb on - strand at 2858.122 kb on - strand at 2858.123 kb on - strand at 2858.123 kb on - strand at 2858.148 kb on + strand at 2858.148 kb on + strand at 2858.148 kb on + strand at 2858.156 kb on - strand at 2858.156 kb on - strand at 2858.156 kb on - strand at 2858.156 kb on - strand at 2858.156 kb on - strand at 2858.156 kb on - strand at 2858.156 kb on - strand at 2858.156 kb on - strand at 2858.167 kb on - strand at 2858.171 kb on + strand at 2858.179 kb on - strand at 2858.230 kb on + strand, within BWI76_RS13695 at 2858.238 kb on - strand, within BWI76_RS13695 at 2858.264 kb on + strand, within BWI76_RS13695 at 2858.270 kb on + strand, within BWI76_RS13695 at 2858.270 kb on + strand, within BWI76_RS13695 at 2858.278 kb on - strand, within BWI76_RS13695 at 2858.310 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.419 kb on + strand, within BWI76_RS13695 at 2858.421 kb on + strand, within BWI76_RS13695 at 2858.421 kb on + strand, within BWI76_RS13695 at 2858.547 kb on + strand at 2858.547 kb on + strand at 2858.547 kb on + strand at 2858.547 kb on + strand at 2858.547 kb on + strand at 2858.547 kb on + strand at 2858.555 kb on - strand at 2858.645 kb on - strand, within BWI76_RS13700 at 2858.665 kb on + strand, within BWI76_RS13700 at 2858.678 kb on + strand, within BWI76_RS13700 at 2858.686 kb on + strand, within BWI76_RS13700 at 2858.688 kb on + strand, within BWI76_RS13700 at 2858.694 kb on - strand, within BWI76_RS13700 at 2858.696 kb on - strand, within BWI76_RS13700 at 2858.745 kb on + strand, within BWI76_RS13700 at 2858.762 kb on + strand, within BWI76_RS13700 at 2858.764 kb on - strand, within BWI76_RS13700 at 2858.772 kb on - strand, within BWI76_RS13700 at 2858.774 kb on + strand, within BWI76_RS13700 at 2858.782 kb on - strand, within BWI76_RS13700 at 2858.787 kb on - strand, within BWI76_RS13700 at 2858.882 kb on - strand, within BWI76_RS13700 at 2858.940 kb on + strand, within BWI76_RS13700 at 2858.950 kb on + strand, within BWI76_RS13700 at 2858.958 kb on - strand, within BWI76_RS13700 at 2858.996 kb on + strand, within BWI76_RS13700 at 2859.041 kb on + strand, within BWI76_RS13700 at 2859.049 kb on - strand, within BWI76_RS13700 at 2859.055 kb on - strand, within BWI76_RS13700 at 2859.056 kb on + strand, within BWI76_RS13700 at 2859.106 kb on - strand, within BWI76_RS13700 at 2859.106 kb on - strand, within BWI76_RS13700 at 2859.106 kb on - strand, within BWI76_RS13700
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,856,585 + BWI76_RS13685 0.76 +0.4 2,856,657 - BWI76_RS13685 0.85 +0.5 2,857,252 + +3.3 2,857,306 + BWI76_RS13690 0.24 -0.9 2,857,314 - BWI76_RS13690 0.25 -0.4 2,857,323 + BWI76_RS13690 0.26 -0.3 2,857,379 + BWI76_RS13690 0.31 +0.2 2,857,387 - BWI76_RS13690 0.31 -0.2 2,857,387 - BWI76_RS13690 0.31 +1.7 2,857,412 + BWI76_RS13690 0.34 -0.3 2,857,457 + BWI76_RS13690 0.38 -0.3 2,857,721 + -0.3 2,857,729 - BWI76_RS13690 0.62 -0.4 2,857,889 + BWI76_RS13690 0.76 +0.2 2,857,978 + BWI76_RS13690 0.84 +0.0 2,857,978 + BWI76_RS13690 0.84 -0.3 2,857,978 + BWI76_RS13690 0.84 +0.6 2,858,003 - BWI76_RS13690 0.87 +0.2 2,858,022 + BWI76_RS13690 0.88 +0.3 2,858,059 + -0.6 2,858,067 - -0.5 2,858,077 - -0.3 2,858,082 - +0.1 2,858,082 - -0.8 2,858,100 - -1.8 2,858,104 + -0.1 2,858,104 + -0.5 2,858,104 + +1.1 2,858,104 + -0.6 2,858,104 + -1.6 2,858,104 + -0.3 2,858,112 - +0.8 2,858,112 - +0.5 2,858,114 + +0.0 2,858,115 + +0.7 2,858,117 - -1.6 2,858,122 - -1.4 2,858,123 - +0.5 2,858,123 - -2.2 2,858,148 + -1.3 2,858,148 + +0.7 2,858,148 + -1.4 2,858,156 - -1.9 2,858,156 - -2.4 2,858,156 - +0.1 2,858,156 - +1.7 2,858,156 - -2.9 2,858,156 - +1.1 2,858,156 - -1.4 2,858,156 - -2.8 2,858,167 - -2.4 2,858,171 + -0.1 2,858,179 - -3.2 2,858,230 + BWI76_RS13695 0.22 -1.9 2,858,238 - BWI76_RS13695 0.25 -0.3 2,858,264 + BWI76_RS13695 0.34 -0.9 2,858,270 + BWI76_RS13695 0.36 -3.9 2,858,270 + BWI76_RS13695 0.36 +0.1 2,858,278 - BWI76_RS13695 0.38 -1.1 2,858,310 + BWI76_RS13695 0.49 +0.7 2,858,419 + BWI76_RS13695 0.87 -0.6 2,858,419 + BWI76_RS13695 0.87 +0.5 2,858,419 + BWI76_RS13695 0.87 -2.2 2,858,419 + BWI76_RS13695 0.87 -2.9 2,858,419 + BWI76_RS13695 0.87 +0.4 2,858,419 + BWI76_RS13695 0.87 -2.3 2,858,419 + BWI76_RS13695 0.87 +1.7 2,858,419 + BWI76_RS13695 0.87 -0.1 2,858,419 + +0.7 2,858,419 + BWI76_RS13695 0.87 -1.5 2,858,419 + BWI76_RS13695 0.87 -0.1 2,858,419 + BWI76_RS13695 0.87 +0.1 2,858,419 + BWI76_RS13695 0.87 -1.7 2,858,419 + BWI76_RS13695 0.87 +0.1 2,858,419 + BWI76_RS13695 0.87 +1.1 2,858,421 + BWI76_RS13695 0.88 +0.7 2,858,421 + BWI76_RS13695 0.88 -0.4 2,858,547 + +0.3 2,858,547 + +1.7 2,858,547 + +0.7 2,858,547 + +0.7 2,858,547 + -2.6 2,858,547 + +0.1 2,858,555 - +0.7 2,858,645 - BWI76_RS13700 0.13 -1.4 2,858,665 + BWI76_RS13700 0.15 -1.1 2,858,678 + BWI76_RS13700 0.16 +0.7 2,858,686 + BWI76_RS13700 0.17 -0.3 2,858,688 + BWI76_RS13700 0.17 +0.1 2,858,694 - BWI76_RS13700 0.18 +0.7 2,858,696 - BWI76_RS13700 0.18 +0.1 2,858,745 + BWI76_RS13700 0.24 -0.3 2,858,762 + BWI76_RS13700 0.25 -2.8 2,858,764 - BWI76_RS13700 0.26 +0.7 2,858,772 - BWI76_RS13700 0.26 -0.2 2,858,774 + BWI76_RS13700 0.27 +0.9 2,858,782 - BWI76_RS13700 0.28 +0.7 2,858,787 - BWI76_RS13700 0.28 +1.7 2,858,882 - BWI76_RS13700 0.38 -0.7 2,858,940 + BWI76_RS13700 0.45 +1.5 2,858,950 + BWI76_RS13700 0.46 -0.9 2,858,958 - BWI76_RS13700 0.46 -0.9 2,858,996 + BWI76_RS13700 0.51 -0.2 2,859,041 + BWI76_RS13700 0.55 +1.7 2,859,049 - BWI76_RS13700 0.56 -0.1 2,859,055 - BWI76_RS13700 0.57 +1.3 2,859,056 + BWI76_RS13700 0.57 +0.0 2,859,106 - BWI76_RS13700 0.62 -0.9 2,859,106 - BWI76_RS13700 0.62 +0.7 2,859,106 - BWI76_RS13700 0.62 -0.3
Or see this region's nucleotide sequence