Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09975 and BWI76_RS09980 are separated by 462 nucleotides BWI76_RS09980 and BWI76_RS09985 overlap by 13 nucleotides
BWI76_RS09975: BWI76_RS09975 - cell division inhibitor SulA, at 2,099,646 to 2,100,155
_RS09975
BWI76_RS09980: BWI76_RS09980 - competence protein, at 2,100,618 to 2,101,241
_RS09980
BWI76_RS09985: BWI76_RS09985 - membrane protein, at 2,101,229 to 2,103,364
_RS09985
Position (kb)
2100
2101
2102 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2099.723 kb on + strand, within BWI76_RS09975 at 2099.724 kb on + strand, within BWI76_RS09975 at 2099.725 kb on + strand, within BWI76_RS09975 at 2099.797 kb on - strand, within BWI76_RS09975 at 2099.893 kb on + strand, within BWI76_RS09975 at 2099.893 kb on + strand, within BWI76_RS09975 at 2099.901 kb on - strand, within BWI76_RS09975 at 2100.118 kb on - strand at 2100.153 kb on - strand at 2100.191 kb on + strand at 2100.206 kb on + strand at 2100.281 kb on + strand at 2100.302 kb on - strand at 2100.370 kb on + strand at 2100.370 kb on + strand at 2100.370 kb on - strand at 2100.375 kb on - strand at 2100.378 kb on - strand at 2100.378 kb on - strand at 2100.378 kb on - strand at 2100.378 kb on - strand at 2100.378 kb on - strand at 2100.378 kb on - strand at 2100.378 kb on - strand at 2100.385 kb on + strand at 2100.388 kb on - strand at 2100.388 kb on - strand at 2100.390 kb on + strand at 2100.398 kb on + strand at 2100.398 kb on - strand at 2100.398 kb on - strand at 2100.398 kb on - strand at 2100.398 kb on - strand at 2100.399 kb on - strand at 2100.412 kb on - strand at 2100.446 kb on + strand at 2100.446 kb on + strand at 2100.510 kb on - strand at 2100.523 kb on + strand at 2100.640 kb on + strand at 2100.640 kb on + strand at 2100.640 kb on + strand at 2100.645 kb on + strand at 2100.648 kb on - strand at 2100.650 kb on + strand at 2100.650 kb on + strand at 2100.650 kb on + strand at 2100.650 kb on + strand at 2100.650 kb on + strand at 2100.658 kb on - strand at 2100.658 kb on - strand at 2100.668 kb on - strand at 2100.669 kb on - strand at 2100.669 kb on - strand at 2100.669 kb on - strand at 2100.705 kb on + strand, within BWI76_RS09980 at 2100.706 kb on + strand, within BWI76_RS09980 at 2100.706 kb on + strand, within BWI76_RS09980 at 2100.706 kb on + strand at 2100.711 kb on + strand, within BWI76_RS09980 at 2100.714 kb on - strand, within BWI76_RS09980 at 2100.714 kb on - strand, within BWI76_RS09980 at 2100.714 kb on - strand, within BWI76_RS09980 at 2100.714 kb on - strand, within BWI76_RS09980 at 2100.714 kb on - strand, within BWI76_RS09980 at 2100.720 kb on + strand, within BWI76_RS09980 at 2100.730 kb on - strand, within BWI76_RS09980 at 2100.772 kb on + strand, within BWI76_RS09980 at 2100.784 kb on + strand, within BWI76_RS09980 at 2100.791 kb on + strand, within BWI76_RS09980 at 2100.791 kb on + strand, within BWI76_RS09980 at 2100.791 kb on + strand, within BWI76_RS09980 at 2100.791 kb on + strand, within BWI76_RS09980 at 2100.794 kb on - strand, within BWI76_RS09980 at 2100.794 kb on - strand, within BWI76_RS09980 at 2100.799 kb on - strand, within BWI76_RS09980 at 2100.799 kb on - strand, within BWI76_RS09980 at 2100.799 kb on - strand, within BWI76_RS09980 at 2100.838 kb on + strand, within BWI76_RS09980 at 2100.900 kb on - strand, within BWI76_RS09980 at 2100.921 kb on + strand, within BWI76_RS09980 at 2100.927 kb on - strand, within BWI76_RS09980 at 2101.005 kb on + strand, within BWI76_RS09980 at 2101.005 kb on + strand, within BWI76_RS09980 at 2101.005 kb on + strand, within BWI76_RS09980 at 2101.005 kb on + strand, within BWI76_RS09980 at 2101.005 kb on + strand, within BWI76_RS09980 at 2101.005 kb on + strand, within BWI76_RS09980 at 2101.005 kb on + strand, within BWI76_RS09980 at 2101.005 kb on + strand, within BWI76_RS09980 at 2101.013 kb on - strand, within BWI76_RS09980 at 2101.013 kb on - strand, within BWI76_RS09980 at 2101.038 kb on + strand, within BWI76_RS09980 at 2101.039 kb on + strand, within BWI76_RS09980 at 2101.039 kb on + strand, within BWI76_RS09980 at 2101.039 kb on + strand, within BWI76_RS09980 at 2101.039 kb on + strand, within BWI76_RS09980 at 2101.039 kb on + strand, within BWI76_RS09980 at 2101.047 kb on - strand at 2101.049 kb on + strand, within BWI76_RS09980 at 2101.049 kb on + strand, within BWI76_RS09980 at 2101.049 kb on + strand, within BWI76_RS09980 at 2101.049 kb on + strand, within BWI76_RS09980 at 2101.049 kb on + strand, within BWI76_RS09980 at 2101.049 kb on + strand, within BWI76_RS09980 at 2101.049 kb on + strand, within BWI76_RS09980 at 2101.057 kb on - strand, within BWI76_RS09980 at 2101.057 kb on - strand, within BWI76_RS09980 at 2101.057 kb on - strand, within BWI76_RS09980 at 2101.065 kb on + strand, within BWI76_RS09980 at 2101.065 kb on + strand, within BWI76_RS09980 at 2101.065 kb on + strand, within BWI76_RS09980 at 2101.075 kb on + strand, within BWI76_RS09980 at 2101.075 kb on + strand, within BWI76_RS09980 at 2101.126 kb on + strand, within BWI76_RS09980 at 2101.130 kb on + strand, within BWI76_RS09980 at 2101.137 kb on + strand, within BWI76_RS09980 at 2101.147 kb on + strand, within BWI76_RS09980 at 2101.155 kb on - strand, within BWI76_RS09980 at 2101.155 kb on - strand, within BWI76_RS09980 at 2101.163 kb on + strand, within BWI76_RS09980 at 2101.170 kb on + strand, within BWI76_RS09980 at 2101.186 kb on + strand at 2101.187 kb on + strand at 2101.188 kb on + strand at 2101.194 kb on - strand at 2101.196 kb on - strand at 2101.211 kb on - strand at 2101.292 kb on - strand at 2101.320 kb on + strand at 2101.404 kb on + strand at 2101.625 kb on + strand, within BWI76_RS09985 at 2101.625 kb on + strand at 2101.657 kb on - strand, within BWI76_RS09985 at 2101.804 kb on - strand, within BWI76_RS09985 at 2101.851 kb on + strand, within BWI76_RS09985 at 2101.913 kb on + strand, within BWI76_RS09985 at 2102.174 kb on + strand, within BWI76_RS09985 at 2102.182 kb on - strand, within BWI76_RS09985 at 2102.197 kb on - strand, within BWI76_RS09985
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,099,723 + BWI76_RS09975 0.15 -0.4 2,099,724 + BWI76_RS09975 0.15 +0.6 2,099,725 + BWI76_RS09975 0.15 +1.1 2,099,797 - BWI76_RS09975 0.30 +0.7 2,099,893 + BWI76_RS09975 0.48 +0.3 2,099,893 + BWI76_RS09975 0.48 -0.6 2,099,901 - BWI76_RS09975 0.50 +0.0 2,100,118 - -0.4 2,100,153 - +1.3 2,100,191 + -0.8 2,100,206 + +0.4 2,100,281 + +0.0 2,100,302 - -1.6 2,100,370 + -2.9 2,100,370 + +1.0 2,100,370 - +1.6 2,100,375 - +1.6 2,100,378 - -1.2 2,100,378 - +0.0 2,100,378 - -3.0 2,100,378 - -0.2 2,100,378 - +0.6 2,100,378 - -1.9 2,100,378 - -0.8 2,100,385 + -2.2 2,100,388 - -2.8 2,100,388 - -0.7 2,100,390 + +0.4 2,100,398 + -1.9 2,100,398 - -0.2 2,100,398 - -0.4 2,100,398 - -0.7 2,100,398 - +0.0 2,100,399 - +1.6 2,100,412 - +0.1 2,100,446 + +1.1 2,100,446 + -0.4 2,100,510 - -1.0 2,100,523 + +0.5 2,100,640 + -0.5 2,100,640 + +1.9 2,100,640 + -0.6 2,100,645 + +0.2 2,100,648 - -1.2 2,100,650 + -0.7 2,100,650 + -0.4 2,100,650 + +1.4 2,100,650 + -1.9 2,100,650 + -0.7 2,100,658 - +1.6 2,100,658 - +1.6 2,100,668 - -0.7 2,100,669 - +1.6 2,100,669 - +0.6 2,100,669 - -1.6 2,100,705 + BWI76_RS09980 0.14 +1.4 2,100,706 + BWI76_RS09980 0.14 -2.0 2,100,706 + BWI76_RS09980 0.14 -2.2 2,100,706 + +1.0 2,100,711 + BWI76_RS09980 0.15 +0.2 2,100,714 - BWI76_RS09980 0.15 +0.0 2,100,714 - BWI76_RS09980 0.15 +1.6 2,100,714 - BWI76_RS09980 0.15 -0.4 2,100,714 - BWI76_RS09980 0.15 +0.9 2,100,714 - BWI76_RS09980 0.15 -0.2 2,100,720 + BWI76_RS09980 0.16 +0.3 2,100,730 - BWI76_RS09980 0.18 -1.2 2,100,772 + BWI76_RS09980 0.25 -1.2 2,100,784 + BWI76_RS09980 0.27 -1.2 2,100,791 + BWI76_RS09980 0.28 +1.3 2,100,791 + BWI76_RS09980 0.28 +1.6 2,100,791 + BWI76_RS09980 0.28 +0.4 2,100,791 + BWI76_RS09980 0.28 +0.3 2,100,794 - BWI76_RS09980 0.28 -1.2 2,100,794 - BWI76_RS09980 0.28 +1.9 2,100,799 - BWI76_RS09980 0.29 +0.6 2,100,799 - BWI76_RS09980 0.29 -1.5 2,100,799 - BWI76_RS09980 0.29 -0.5 2,100,838 + BWI76_RS09980 0.35 -2.3 2,100,900 - BWI76_RS09980 0.45 +1.0 2,100,921 + BWI76_RS09980 0.49 +1.0 2,100,927 - BWI76_RS09980 0.50 -0.4 2,101,005 + BWI76_RS09980 0.62 -0.5 2,101,005 + BWI76_RS09980 0.62 -0.3 2,101,005 + BWI76_RS09980 0.62 -1.4 2,101,005 + BWI76_RS09980 0.62 +0.1 2,101,005 + BWI76_RS09980 0.62 +0.4 2,101,005 + BWI76_RS09980 0.62 -0.2 2,101,005 + BWI76_RS09980 0.62 -1.0 2,101,005 + BWI76_RS09980 0.62 +0.2 2,101,013 - BWI76_RS09980 0.63 +0.4 2,101,013 - BWI76_RS09980 0.63 -1.2 2,101,038 + BWI76_RS09980 0.67 -0.5 2,101,039 + BWI76_RS09980 0.67 -0.9 2,101,039 + BWI76_RS09980 0.67 +1.1 2,101,039 + BWI76_RS09980 0.67 -0.9 2,101,039 + BWI76_RS09980 0.67 -1.2 2,101,039 + BWI76_RS09980 0.67 -2.2 2,101,047 - -0.4 2,101,049 + BWI76_RS09980 0.69 -1.7 2,101,049 + BWI76_RS09980 0.69 +1.0 2,101,049 + BWI76_RS09980 0.69 -0.7 2,101,049 + BWI76_RS09980 0.69 +0.3 2,101,049 + BWI76_RS09980 0.69 +0.3 2,101,049 + BWI76_RS09980 0.69 -0.0 2,101,049 + BWI76_RS09980 0.69 -0.7 2,101,057 - BWI76_RS09980 0.70 -1.4 2,101,057 - BWI76_RS09980 0.70 +0.0 2,101,057 - BWI76_RS09980 0.70 -3.2 2,101,065 + BWI76_RS09980 0.72 +2.3 2,101,065 + BWI76_RS09980 0.72 +1.6 2,101,065 + BWI76_RS09980 0.72 -0.4 2,101,075 + BWI76_RS09980 0.73 +1.3 2,101,075 + BWI76_RS09980 0.73 -0.8 2,101,126 + BWI76_RS09980 0.81 +0.1 2,101,130 + BWI76_RS09980 0.82 -2.3 2,101,137 + BWI76_RS09980 0.83 +1.6 2,101,147 + BWI76_RS09980 0.85 -0.4 2,101,155 - BWI76_RS09980 0.86 -1.9 2,101,155 - BWI76_RS09980 0.86 -0.1 2,101,163 + BWI76_RS09980 0.87 +0.4 2,101,170 + BWI76_RS09980 0.88 -2.0 2,101,186 + -0.3 2,101,187 + +0.0 2,101,188 + -1.2 2,101,194 - +0.2 2,101,196 - -0.3 2,101,211 - +0.2 2,101,292 - -0.0 2,101,320 + -0.7 2,101,404 + +0.4 2,101,625 + BWI76_RS09985 0.19 +0.4 2,101,625 + -0.2 2,101,657 - BWI76_RS09985 0.20 -1.3 2,101,804 - BWI76_RS09985 0.27 +0.6 2,101,851 + BWI76_RS09985 0.29 -2.5 2,101,913 + BWI76_RS09985 0.32 +0.6 2,102,174 + BWI76_RS09985 0.44 -1.2 2,102,182 - BWI76_RS09985 0.45 -0.3 2,102,197 - BWI76_RS09985 0.45 -0.8
Or see this region's nucleotide sequence