Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04395 and BWI76_RS04400 are separated by 92 nucleotides BWI76_RS04400 and BWI76_RS04405 are separated by 44 nucleotides BWI76_RS04405 and BWI76_RS04410 overlap by 8 nucleotides
BWI76_RS04395: BWI76_RS04395 - putative HTH-type transcriptional regulator, at 906,931 to 907,875
_RS04395
BWI76_RS04400: BWI76_RS04400 - alanyl-tRNA synthetase, at 907,968 to 908,597
_RS04400
BWI76_RS04405: BWI76_RS04405 - response regulator, at 908,642 to 909,340
_RS04405
BWI76_RS04410: BWI76_RS04410 - signal transduction histidine kinase regulating citrate/malate metabolism, at 909,333 to 910,961
_RS04410
Position (kb) 908
909
910 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 907.735 kb on + strand, within BWI76_RS04395 at 907.749 kb on - strand, within BWI76_RS04395 at 907.957 kb on + strand at 907.964 kb on + strand at 908.164 kb on + strand, within BWI76_RS04400 at 908.177 kb on - strand, within BWI76_RS04400 at 908.247 kb on + strand, within BWI76_RS04400 at 908.273 kb on + strand at 908.321 kb on - strand, within BWI76_RS04400 at 908.336 kb on - strand, within BWI76_RS04400 at 908.365 kb on - strand, within BWI76_RS04400 at 908.497 kb on - strand, within BWI76_RS04400 at 908.509 kb on + strand, within BWI76_RS04400 at 908.595 kb on - strand at 908.740 kb on + strand, within BWI76_RS04405 at 908.753 kb on - strand, within BWI76_RS04405 at 908.753 kb on - strand, within BWI76_RS04405 at 908.836 kb on - strand, within BWI76_RS04405 at 908.843 kb on + strand, within BWI76_RS04405 at 908.850 kb on - strand, within BWI76_RS04405 at 908.855 kb on + strand, within BWI76_RS04405 at 908.910 kb on + strand, within BWI76_RS04405 at 908.956 kb on + strand, within BWI76_RS04405 at 909.112 kb on + strand, within BWI76_RS04405 at 909.170 kb on - strand, within BWI76_RS04405 at 909.186 kb on + strand, within BWI76_RS04405 at 909.204 kb on - strand, within BWI76_RS04405 at 909.238 kb on - strand, within BWI76_RS04405 at 909.257 kb on - strand, within BWI76_RS04405 at 909.273 kb on + strand at 909.298 kb on + strand at 909.306 kb on - strand at 909.325 kb on + strand at 909.333 kb on - strand at 909.447 kb on - strand at 909.449 kb on + strand at 909.599 kb on + strand, within BWI76_RS04410 at 909.607 kb on - strand, within BWI76_RS04410 at 909.699 kb on + strand, within BWI76_RS04410 at 909.700 kb on - strand, within BWI76_RS04410 at 909.707 kb on - strand, within BWI76_RS04410 at 909.708 kb on - strand, within BWI76_RS04410 at 909.731 kb on + strand, within BWI76_RS04410 at 909.739 kb on - strand, within BWI76_RS04410 at 909.754 kb on + strand, within BWI76_RS04410 at 909.832 kb on - strand, within BWI76_RS04410 at 909.843 kb on - strand, within BWI76_RS04410 at 910.034 kb on - strand, within BWI76_RS04410 at 910.034 kb on - strand, within BWI76_RS04410 at 910.080 kb on - strand, within BWI76_RS04410 at 910.082 kb on + strand, within BWI76_RS04410 at 910.082 kb on + strand, within BWI76_RS04410 at 910.090 kb on - strand, within BWI76_RS04410 at 910.207 kb on - strand, within BWI76_RS04410 at 910.313 kb on + strand, within BWI76_RS04410
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 907,735 + BWI76_RS04395 0.85 +0.1 907,749 - BWI76_RS04395 0.87 -2.8 907,957 + +0.2 907,964 + +0.6 908,164 + BWI76_RS04400 0.31 +0.2 908,177 - BWI76_RS04400 0.33 +1.2 908,247 + BWI76_RS04400 0.44 -0.2 908,273 + -0.8 908,321 - BWI76_RS04400 0.56 -1.1 908,336 - BWI76_RS04400 0.58 +0.6 908,365 - BWI76_RS04400 0.63 -0.7 908,497 - BWI76_RS04400 0.84 +0.6 908,509 + BWI76_RS04400 0.86 -0.1 908,595 - -0.4 908,740 + BWI76_RS04405 0.14 +0.5 908,753 - BWI76_RS04405 0.16 +0.6 908,753 - BWI76_RS04405 0.16 +0.2 908,836 - BWI76_RS04405 0.28 +0.8 908,843 + BWI76_RS04405 0.29 -0.4 908,850 - BWI76_RS04405 0.30 -2.9 908,855 + BWI76_RS04405 0.30 +0.2 908,910 + BWI76_RS04405 0.38 -0.5 908,956 + BWI76_RS04405 0.45 -0.7 909,112 + BWI76_RS04405 0.67 -0.4 909,170 - BWI76_RS04405 0.76 +0.0 909,186 + BWI76_RS04405 0.78 +0.5 909,204 - BWI76_RS04405 0.80 -0.1 909,238 - BWI76_RS04405 0.85 +0.3 909,257 - BWI76_RS04405 0.88 +0.4 909,273 + +0.2 909,298 + -0.8 909,306 - +0.7 909,325 + +0.5 909,333 - -0.1 909,447 - -1.5 909,449 + -0.4 909,599 + BWI76_RS04410 0.16 +0.3 909,607 - BWI76_RS04410 0.17 +1.1 909,699 + BWI76_RS04410 0.22 -0.0 909,700 - BWI76_RS04410 0.23 +0.6 909,707 - BWI76_RS04410 0.23 +1.6 909,708 - BWI76_RS04410 0.23 +0.3 909,731 + BWI76_RS04410 0.24 -1.9 909,739 - BWI76_RS04410 0.25 -0.4 909,754 + BWI76_RS04410 0.26 -0.8 909,832 - BWI76_RS04410 0.31 +0.0 909,843 - BWI76_RS04410 0.31 +0.4 910,034 - BWI76_RS04410 0.43 -1.4 910,034 - BWI76_RS04410 0.43 +0.5 910,080 - BWI76_RS04410 0.46 +0.1 910,082 + BWI76_RS04410 0.46 +0.6 910,082 + BWI76_RS04410 0.46 +1.1 910,090 - BWI76_RS04410 0.46 +0.6 910,207 - BWI76_RS04410 0.54 +0.3 910,313 + BWI76_RS04410 0.60 +0.2
Or see this region's nucleotide sequence