Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20870 and BWI76_RS20875 are separated by 27 nucleotides BWI76_RS20875 and BWI76_RS20880 are separated by 232 nucleotides BWI76_RS20880 and BWI76_RS20885 are separated by 77 nucleotides
BWI76_RS20870: BWI76_RS20870 - succinyl-diaminopimelate desuccinylase, at 4,364,225 to 4,365,352
_RS20870
BWI76_RS20875: BWI76_RS20875 - membrane protein, at 4,365,380 to 4,365,583
_RS20875
BWI76_RS20880: BWI76_RS20880 - esterase, at 4,365,816 to 4,366,514
_RS20880
BWI76_RS20885: BWI76_RS20885 - tRNA(Met) cytidine acetyltransferase TmcA, at 4,366,592 to 4,368,592
_RS20885
Position (kb)
4365
4366
4367 Strain fitness (log2 ratio)
-1
0
1 at 4365.337 kb on + strand at 4365.407 kb on + strand, within BWI76_RS20875 at 4366.189 kb on - strand, within BWI76_RS20880 at 4366.394 kb on + strand, within BWI76_RS20880 at 4366.824 kb on - strand at 4366.831 kb on - strand, within BWI76_RS20885 at 4367.071 kb on + strand, within BWI76_RS20885 at 4367.079 kb on - strand, within BWI76_RS20885
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,365,337 + -0.0 4,365,407 + BWI76_RS20875 0.13 +0.2 4,366,189 - BWI76_RS20880 0.53 +0.5 4,366,394 + BWI76_RS20880 0.83 +0.9 4,366,824 - +0.7 4,366,831 - BWI76_RS20885 0.12 +0.6 4,367,071 + BWI76_RS20885 0.24 -0.8 4,367,079 - BWI76_RS20885 0.24 +1.7
Or see this region's nucleotide sequence