Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17970 and BWI76_RS17975 are separated by 236 nucleotides BWI76_RS17975 and BWI76_RS17980 are separated by 19 nucleotides BWI76_RS17980 and BWI76_RS17985 are separated by 97 nucleotides
BWI76_RS17970: BWI76_RS17970 - zinc metalloprotease HtpX, at 3,732,923 to 3,733,804
_RS17970
BWI76_RS17975: BWI76_RS17975 - tail-specific protease, at 3,734,041 to 3,736,089
_RS17975
BWI76_RS17980: BWI76_RS17980 - RNA chaperone ProQ, at 3,736,109 to 3,736,792
_RS17980
BWI76_RS17985: BWI76_RS17985 - hypothetical protein, at 3,736,890 to 3,737,387
_RS17985
Position (kb)
3734
3735
3736
3737 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3733.496 kb on + strand, within BWI76_RS17970 at 3734.532 kb on + strand, within BWI76_RS17975 at 3734.577 kb on + strand, within BWI76_RS17975 at 3734.611 kb on - strand, within BWI76_RS17975 at 3734.890 kb on - strand, within BWI76_RS17975 at 3735.093 kb on + strand, within BWI76_RS17975 at 3735.745 kb on - strand, within BWI76_RS17975 at 3735.988 kb on - strand at 3736.159 kb on + strand at 3736.446 kb on + strand, within BWI76_RS17980 at 3736.446 kb on + strand, within BWI76_RS17980 at 3736.454 kb on - strand, within BWI76_RS17980 at 3736.575 kb on - strand, within BWI76_RS17980 at 3736.575 kb on - strand, within BWI76_RS17980 at 3736.582 kb on + strand, within BWI76_RS17980 at 3736.871 kb on + strand at 3736.871 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,733,496 + BWI76_RS17970 0.65 -0.4 3,734,532 + BWI76_RS17975 0.24 -4.0 3,734,577 + BWI76_RS17975 0.26 -0.4 3,734,611 - BWI76_RS17975 0.28 -3.8 3,734,890 - BWI76_RS17975 0.41 -2.4 3,735,093 + BWI76_RS17975 0.51 -2.0 3,735,745 - BWI76_RS17975 0.83 -3.3 3,735,988 - -4.0 3,736,159 + +0.6 3,736,446 + BWI76_RS17980 0.49 -3.7 3,736,446 + BWI76_RS17980 0.49 -3.7 3,736,454 - BWI76_RS17980 0.50 -2.0 3,736,575 - BWI76_RS17980 0.68 -2.2 3,736,575 - BWI76_RS17980 0.68 -2.8 3,736,582 + BWI76_RS17980 0.69 -3.2 3,736,871 + -0.5 3,736,871 + +0.4
Or see this region's nucleotide sequence