Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17975

Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17970 and BWI76_RS17975 are separated by 236 nucleotidesBWI76_RS17975 and BWI76_RS17980 are separated by 19 nucleotidesBWI76_RS17980 and BWI76_RS17985 are separated by 97 nucleotides BWI76_RS17970: BWI76_RS17970 - zinc metalloprotease HtpX, at 3,732,923 to 3,733,804 _RS17970 BWI76_RS17975: BWI76_RS17975 - tail-specific protease, at 3,734,041 to 3,736,089 _RS17975 BWI76_RS17980: BWI76_RS17980 - RNA chaperone ProQ, at 3,736,109 to 3,736,792 _RS17980 BWI76_RS17985: BWI76_RS17985 - hypothetical protein, at 3,736,890 to 3,737,387 _RS17985 Position (kb) 3734 3735 3736 3737Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3733.496 kb on + strand, within BWI76_RS17970at 3734.532 kb on + strand, within BWI76_RS17975at 3734.577 kb on + strand, within BWI76_RS17975at 3734.611 kb on - strand, within BWI76_RS17975at 3734.890 kb on - strand, within BWI76_RS17975at 3735.093 kb on + strand, within BWI76_RS17975at 3735.745 kb on - strand, within BWI76_RS17975at 3735.988 kb on - strandat 3736.159 kb on + strandat 3736.446 kb on + strand, within BWI76_RS17980at 3736.446 kb on + strand, within BWI76_RS17980at 3736.454 kb on - strand, within BWI76_RS17980at 3736.575 kb on - strand, within BWI76_RS17980at 3736.575 kb on - strand, within BWI76_RS17980at 3736.582 kb on + strand, within BWI76_RS17980at 3736.871 kb on + strandat 3736.871 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,733,496 + BWI76_RS17970 0.65 -0.4
3,734,532 + BWI76_RS17975 0.24 -4.0
3,734,577 + BWI76_RS17975 0.26 -0.4
3,734,611 - BWI76_RS17975 0.28 -3.8
3,734,890 - BWI76_RS17975 0.41 -2.4
3,735,093 + BWI76_RS17975 0.51 -2.0
3,735,745 - BWI76_RS17975 0.83 -3.3
3,735,988 - -4.0
3,736,159 + +0.6
3,736,446 + BWI76_RS17980 0.49 -3.7
3,736,446 + BWI76_RS17980 0.49 -3.7
3,736,454 - BWI76_RS17980 0.50 -2.0
3,736,575 - BWI76_RS17980 0.68 -2.2
3,736,575 - BWI76_RS17980 0.68 -2.8
3,736,582 + BWI76_RS17980 0.69 -3.2
3,736,871 + -0.5
3,736,871 + +0.4

Or see this region's nucleotide sequence