Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16135 and BWI76_RS16140 are separated by 10 nucleotides BWI76_RS16140 and BWI76_RS16145 are separated by 10 nucleotides BWI76_RS16145 and BWI76_RS16150 are separated by 167 nucleotides
BWI76_RS16135: BWI76_RS16135 - acetyl-CoA acetyltransferase, at 3,347,750 to 3,348,952
_RS16135
BWI76_RS16140: BWI76_RS16140 - 3-oxoadipate CoA-transferase, at 3,348,963 to 3,349,619
_RS16140
BWI76_RS16145: BWI76_RS16145 - 3-oxoadipate CoA-transferase, at 3,349,630 to 3,350,316
_RS16145
BWI76_RS16150: BWI76_RS16150 - IclR family transcriptional regulator, at 3,350,484 to 3,351,290
_RS16150
Position (kb) 3348
3349
3350 Strain fitness (log2 ratio) -1
0
1 at 3348.604 kb on - strand, within BWI76_RS16135 at 3349.004 kb on + strand at 3349.401 kb on - strand, within BWI76_RS16140 at 3349.411 kb on + strand, within BWI76_RS16140 at 3350.569 kb on + strand, within BWI76_RS16150 at 3350.577 kb on - strand, within BWI76_RS16150
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,348,604 - BWI76_RS16135 0.71 +0.7 3,349,004 + +0.7 3,349,401 - BWI76_RS16140 0.67 +1.0 3,349,411 + BWI76_RS16140 0.68 +0.1 3,350,569 + BWI76_RS16150 0.11 -0.0 3,350,577 - BWI76_RS16150 0.12 -0.9
Or see this region's nucleotide sequence