Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15520 and BWI76_RS15525 are separated by 86 nucleotides BWI76_RS15525 and BWI76_RS15530 overlap by 14 nucleotides BWI76_RS15530 and BWI76_RS15535 overlap by 4 nucleotides BWI76_RS15535 and BWI76_RS15540 overlap by 1 nucleotides
BWI76_RS15520: BWI76_RS15520 - hypothetical protein, at 3,250,770 to 3,252,617
_RS15520
BWI76_RS15525: BWI76_RS15525 - hypothetical protein, at 3,252,704 to 3,255,181
_RS15525
BWI76_RS15530: BWI76_RS15530 - hypothetical protein, at 3,255,168 to 3,255,563
_RS15530
BWI76_RS15535: BWI76_RS15535 - hypothetical protein, at 3,255,560 to 3,256,030
_RS15535
BWI76_RS15540: BWI76_RS15540 - hypothetical protein, at 3,256,030 to 3,256,506
_RS15540
Position (kb)
3252
3253
3254
3255
3256 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3251.712 kb on - strand, within BWI76_RS15520 at 3251.737 kb on + strand, within BWI76_RS15520 at 3251.745 kb on - strand, within BWI76_RS15520 at 3251.770 kb on - strand, within BWI76_RS15520 at 3251.770 kb on - strand, within BWI76_RS15520 at 3251.864 kb on + strand, within BWI76_RS15520 at 3251.893 kb on - strand, within BWI76_RS15520 at 3251.893 kb on - strand, within BWI76_RS15520 at 3251.916 kb on + strand, within BWI76_RS15520 at 3251.919 kb on + strand, within BWI76_RS15520 at 3251.919 kb on - strand, within BWI76_RS15520 at 3251.927 kb on - strand, within BWI76_RS15520 at 3251.929 kb on + strand, within BWI76_RS15520 at 3251.937 kb on - strand, within BWI76_RS15520 at 3251.961 kb on + strand, within BWI76_RS15520 at 3251.985 kb on - strand, within BWI76_RS15520 at 3252.020 kb on - strand, within BWI76_RS15520 at 3252.025 kb on - strand, within BWI76_RS15520 at 3252.030 kb on + strand, within BWI76_RS15520 at 3252.048 kb on - strand, within BWI76_RS15520 at 3252.314 kb on + strand, within BWI76_RS15520 at 3252.390 kb on - strand, within BWI76_RS15520 at 3252.436 kb on + strand at 3252.444 kb on - strand at 3252.452 kb on + strand at 3252.460 kb on - strand at 3252.469 kb on + strand at 3252.477 kb on - strand at 3252.517 kb on - strand at 3252.519 kb on - strand at 3252.523 kb on + strand at 3252.525 kb on - strand at 3252.543 kb on + strand at 3252.551 kb on - strand at 3252.683 kb on - strand at 3252.766 kb on + strand at 3252.774 kb on - strand at 3252.795 kb on - strand at 3252.805 kb on - strand at 3253.001 kb on + strand, within BWI76_RS15525 at 3253.051 kb on + strand, within BWI76_RS15525 at 3253.056 kb on + strand, within BWI76_RS15525 at 3253.069 kb on + strand, within BWI76_RS15525 at 3253.162 kb on + strand at 3253.170 kb on - strand, within BWI76_RS15525 at 3253.314 kb on + strand, within BWI76_RS15525 at 3253.334 kb on + strand, within BWI76_RS15525 at 3253.489 kb on - strand, within BWI76_RS15525 at 3253.520 kb on - strand, within BWI76_RS15525 at 3254.115 kb on + strand, within BWI76_RS15525 at 3254.120 kb on + strand, within BWI76_RS15525 at 3254.199 kb on - strand, within BWI76_RS15525 at 3254.204 kb on - strand, within BWI76_RS15525 at 3254.209 kb on - strand, within BWI76_RS15525 at 3254.622 kb on + strand, within BWI76_RS15525 at 3254.692 kb on + strand, within BWI76_RS15525 at 3254.700 kb on - strand, within BWI76_RS15525 at 3254.829 kb on + strand, within BWI76_RS15525 at 3255.017 kb on - strand at 3255.432 kb on + strand, within BWI76_RS15530 at 3255.517 kb on + strand at 3255.517 kb on + strand, within BWI76_RS15530 at 3255.525 kb on - strand at 3255.525 kb on - strand at 3255.555 kb on - strand at 3255.574 kb on + strand at 3255.574 kb on + strand at 3255.582 kb on - strand at 3255.582 kb on - strand at 3255.642 kb on - strand, within BWI76_RS15535 at 3255.854 kb on - strand, within BWI76_RS15535 at 3255.979 kb on + strand, within BWI76_RS15535 at 3255.987 kb on - strand at 3256.015 kb on + strand at 3256.131 kb on + strand, within BWI76_RS15540
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,251,712 - BWI76_RS15520 0.51 -0.9 3,251,737 + BWI76_RS15520 0.52 -1.2 3,251,745 - BWI76_RS15520 0.53 -2.0 3,251,770 - BWI76_RS15520 0.54 -3.7 3,251,770 - BWI76_RS15520 0.54 -0.2 3,251,864 + BWI76_RS15520 0.59 -0.3 3,251,893 - BWI76_RS15520 0.61 -1.8 3,251,893 - BWI76_RS15520 0.61 -1.2 3,251,916 + BWI76_RS15520 0.62 -0.7 3,251,919 + BWI76_RS15520 0.62 -1.4 3,251,919 - BWI76_RS15520 0.62 -0.3 3,251,927 - BWI76_RS15520 0.63 -0.9 3,251,929 + BWI76_RS15520 0.63 -0.5 3,251,937 - BWI76_RS15520 0.63 -0.5 3,251,961 + BWI76_RS15520 0.64 -1.4 3,251,985 - BWI76_RS15520 0.66 -1.0 3,252,020 - BWI76_RS15520 0.68 -2.2 3,252,025 - BWI76_RS15520 0.68 -0.4 3,252,030 + BWI76_RS15520 0.68 -3.1 3,252,048 - BWI76_RS15520 0.69 -0.6 3,252,314 + BWI76_RS15520 0.84 +0.3 3,252,390 - BWI76_RS15520 0.88 +0.7 3,252,436 + -0.4 3,252,444 - +1.1 3,252,452 + -0.6 3,252,460 - -0.4 3,252,469 + -0.9 3,252,477 - -0.3 3,252,517 - -1.4 3,252,519 - -1.0 3,252,523 + -0.2 3,252,525 - -0.7 3,252,543 + -0.7 3,252,551 - -3.2 3,252,683 - -0.1 3,252,766 + -0.6 3,252,774 - -0.6 3,252,795 - -0.4 3,252,805 - -0.6 3,253,001 + BWI76_RS15525 0.12 -1.5 3,253,051 + BWI76_RS15525 0.14 -0.8 3,253,056 + BWI76_RS15525 0.14 -1.8 3,253,069 + BWI76_RS15525 0.15 -2.1 3,253,162 + +0.7 3,253,170 - BWI76_RS15525 0.19 -1.1 3,253,314 + BWI76_RS15525 0.25 -1.3 3,253,334 + BWI76_RS15525 0.25 -1.6 3,253,489 - BWI76_RS15525 0.32 -0.5 3,253,520 - BWI76_RS15525 0.33 +0.1 3,254,115 + BWI76_RS15525 0.57 -2.1 3,254,120 + BWI76_RS15525 0.57 -2.6 3,254,199 - BWI76_RS15525 0.60 -0.9 3,254,204 - BWI76_RS15525 0.61 -0.3 3,254,209 - BWI76_RS15525 0.61 -0.2 3,254,622 + BWI76_RS15525 0.77 -0.4 3,254,692 + BWI76_RS15525 0.80 -0.3 3,254,700 - BWI76_RS15525 0.81 -0.2 3,254,829 + BWI76_RS15525 0.86 -0.6 3,255,017 - -1.4 3,255,432 + BWI76_RS15530 0.67 -1.1 3,255,517 + -0.3 3,255,517 + BWI76_RS15530 0.88 +0.6 3,255,525 - -0.8 3,255,525 - -0.4 3,255,555 - -0.2 3,255,574 + +0.5 3,255,574 + +0.1 3,255,582 - -0.0 3,255,582 - -0.7 3,255,642 - BWI76_RS15535 0.17 -0.4 3,255,854 - BWI76_RS15535 0.62 -1.1 3,255,979 + BWI76_RS15535 0.89 -1.1 3,255,987 - -1.2 3,256,015 + -0.6 3,256,131 + BWI76_RS15540 0.21 -4.0
Or see this region's nucleotide sequence