Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13065 and BWI76_RS13070 are separated by 47 nucleotides BWI76_RS13070 and BWI76_RS13075 are separated by 231 nucleotides BWI76_RS13075 and BWI76_RS13080 are separated by 176 nucleotides
BWI76_RS13065: BWI76_RS13065 - LysR family transcriptional regulator, at 2,725,118 to 2,725,993
_RS13065
BWI76_RS13070: BWI76_RS13070 - hypothetical protein, at 2,726,041 to 2,726,268
_RS13070
BWI76_RS13075: BWI76_RS13075 - cupin, at 2,726,500 to 2,726,832
_RS13075
BWI76_RS13080: BWI76_RS13080 - tyramine oxidase, at 2,727,009 to 2,729,267
_RS13080
Position (kb)
2726
2727 Strain fitness (log2 ratio)
-1
0
1 at 2725.215 kb on - strand, within BWI76_RS13065 at 2725.217 kb on + strand, within BWI76_RS13065 at 2725.261 kb on + strand, within BWI76_RS13065 at 2725.342 kb on + strand, within BWI76_RS13065 at 2725.387 kb on - strand, within BWI76_RS13065 at 2725.668 kb on + strand, within BWI76_RS13065 at 2726.069 kb on - strand, within BWI76_RS13070 at 2726.205 kb on - strand, within BWI76_RS13070 at 2727.014 kb on + strand at 2727.138 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,725,215 - BWI76_RS13065 0.11 +0.4 2,725,217 + BWI76_RS13065 0.11 -0.0 2,725,261 + BWI76_RS13065 0.16 +1.6 2,725,342 + BWI76_RS13065 0.26 +0.1 2,725,387 - BWI76_RS13065 0.31 -0.5 2,725,668 + BWI76_RS13065 0.63 +0.2 2,726,069 - BWI76_RS13070 0.12 +0.3 2,726,205 - BWI76_RS13070 0.72 +1.0 2,727,014 + +0.4 2,727,138 + +0.3
Or see this region's nucleotide sequence