Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12370 and BWI76_RS12375 are separated by 91 nucleotides BWI76_RS12375 and BWI76_RS12380 are separated by 488 nucleotides BWI76_RS12380 and BWI76_RS12385 are separated by 1 nucleotides
BWI76_RS12370: BWI76_RS12370 - hypothetical protein, at 2,583,369 to 2,585,315
_RS12370
BWI76_RS12375: BWI76_RS12375 - DNA-invertase hin, at 2,585,407 to 2,585,958
_RS12375
BWI76_RS12380: BWI76_RS12380 - DNA polymerase V subunit UmuD, at 2,586,447 to 2,586,866
_RS12380
BWI76_RS12385: BWI76_RS12385 - DNA polymerase V subunit UmuC, at 2,586,868 to 2,588,133
_RS12385
Position (kb)
2585
2586 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2584.559 kb on - strand, within BWI76_RS12370 at 2584.563 kb on + strand, within BWI76_RS12370 at 2584.564 kb on - strand, within BWI76_RS12370 at 2584.773 kb on - strand, within BWI76_RS12370 at 2584.773 kb on - strand, within BWI76_RS12370 at 2584.965 kb on - strand, within BWI76_RS12370 at 2585.108 kb on + strand, within BWI76_RS12370 at 2585.211 kb on + strand at 2585.536 kb on + strand, within BWI76_RS12375 at 2585.614 kb on - strand, within BWI76_RS12375 at 2585.644 kb on - strand, within BWI76_RS12375 at 2585.800 kb on + strand, within BWI76_RS12375 at 2586.058 kb on - strand at 2586.071 kb on + strand at 2586.188 kb on - strand at 2586.460 kb on - strand at 2586.478 kb on + strand at 2586.505 kb on + strand, within BWI76_RS12380 at 2586.547 kb on - strand, within BWI76_RS12380 at 2586.570 kb on - strand, within BWI76_RS12380 at 2586.582 kb on + strand, within BWI76_RS12380 at 2586.687 kb on + strand, within BWI76_RS12380 at 2586.758 kb on + strand, within BWI76_RS12380 at 2586.764 kb on + strand, within BWI76_RS12380 at 2586.825 kb on - strand at 2586.827 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,584,559 - BWI76_RS12370 0.61 -0.6 2,584,563 + BWI76_RS12370 0.61 -1.6 2,584,564 - BWI76_RS12370 0.61 -0.5 2,584,773 - BWI76_RS12370 0.72 -2.2 2,584,773 - BWI76_RS12370 0.72 -0.0 2,584,965 - BWI76_RS12370 0.82 -0.5 2,585,108 + BWI76_RS12370 0.89 +0.5 2,585,211 + +0.0 2,585,536 + BWI76_RS12375 0.23 -1.5 2,585,614 - BWI76_RS12375 0.38 -0.6 2,585,644 - BWI76_RS12375 0.43 -0.0 2,585,800 + BWI76_RS12375 0.71 -1.9 2,586,058 - -1.0 2,586,071 + -0.2 2,586,188 - -1.5 2,586,460 - -1.1 2,586,478 + +0.1 2,586,505 + BWI76_RS12380 0.14 -0.7 2,586,547 - BWI76_RS12380 0.24 -0.3 2,586,570 - BWI76_RS12380 0.29 -1.2 2,586,582 + BWI76_RS12380 0.32 -1.5 2,586,687 + BWI76_RS12380 0.57 +0.2 2,586,758 + BWI76_RS12380 0.74 -3.1 2,586,764 + BWI76_RS12380 0.75 -0.6 2,586,825 - -1.3 2,586,827 + -0.5
Or see this region's nucleotide sequence