Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11440 and BWI76_RS11445 are separated by 162 nucleotides BWI76_RS11445 and BWI76_RS11450 are separated by 37 nucleotides BWI76_RS11450 and BWI76_RS11455 are separated by 100 nucleotides BWI76_RS11455 and BWI76_RS11460 overlap by 17 nucleotides
BWI76_RS11440: BWI76_RS11440 - hypothetical protein, at 2,408,280 to 2,408,789
_RS11440
BWI76_RS11445: BWI76_RS11445 - hypothetical protein, at 2,408,952 to 2,409,644
_RS11445
BWI76_RS11450: BWI76_RS11450 - cyanate MFS transporter, at 2,409,682 to 2,410,866
_RS11450
BWI76_RS11455: BWI76_RS11455 - AraC family transcriptional regulator, at 2,410,967 to 2,411,758
_RS11455
BWI76_RS11460: BWI76_RS11460 - DUF441 family protein, at 2,411,742 to 2,412,188
_RS11460
Position (kb)
2409
2410
2411 Strain fitness (log2 ratio)
-2
-1
0
1 at 2408.829 kb on - strand at 2408.949 kb on + strand at 2409.372 kb on - strand, within BWI76_RS11445 at 2409.379 kb on + strand, within BWI76_RS11445 at 2409.387 kb on - strand, within BWI76_RS11445 at 2409.469 kb on + strand, within BWI76_RS11445 at 2409.606 kb on - strand at 2410.009 kb on + strand, within BWI76_RS11450 at 2410.017 kb on - strand, within BWI76_RS11450 at 2410.579 kb on + strand, within BWI76_RS11450 at 2410.592 kb on - strand, within BWI76_RS11450 at 2410.592 kb on - strand, within BWI76_RS11450 at 2410.623 kb on + strand, within BWI76_RS11450 at 2410.726 kb on + strand, within BWI76_RS11450 at 2410.837 kb on + strand at 2411.331 kb on + strand, within BWI76_RS11455 at 2411.569 kb on - strand, within BWI76_RS11455 at 2411.656 kb on - strand, within BWI76_RS11455 at 2411.861 kb on + strand, within BWI76_RS11460
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,408,829 - +0.5 2,408,949 + +0.4 2,409,372 - BWI76_RS11445 0.61 -0.2 2,409,379 + BWI76_RS11445 0.62 -2.1 2,409,387 - BWI76_RS11445 0.63 +0.6 2,409,469 + BWI76_RS11445 0.75 +0.6 2,409,606 - -0.4 2,410,009 + BWI76_RS11450 0.28 +0.5 2,410,017 - BWI76_RS11450 0.28 +1.1 2,410,579 + BWI76_RS11450 0.76 +1.2 2,410,592 - BWI76_RS11450 0.77 +0.5 2,410,592 - BWI76_RS11450 0.77 +0.5 2,410,623 + BWI76_RS11450 0.79 -1.5 2,410,726 + BWI76_RS11450 0.88 +1.1 2,410,837 + +0.3 2,411,331 + BWI76_RS11455 0.46 +0.1 2,411,569 - BWI76_RS11455 0.76 -2.5 2,411,656 - BWI76_RS11455 0.87 +0.0 2,411,861 + BWI76_RS11460 0.27 +0.1
Or see this region's nucleotide sequence