Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09015 and BWI76_RS09020 are separated by 151 nucleotides BWI76_RS09020 and BWI76_RS09025 are separated by 70 nucleotides BWI76_RS09025 and BWI76_RS09030 are separated by 43 nucleotides
BWI76_RS09015: BWI76_RS09015 - aldo/keto reductase, at 1,898,905 to 1,899,945
_RS09015
BWI76_RS09020: BWI76_RS09020 - 6-phospho-beta-glucosidase, at 1,900,097 to 1,901,473
_RS09020
BWI76_RS09025: BWI76_RS09025 - GntR family transcriptional regulator, at 1,901,544 to 1,902,260
_RS09025
BWI76_RS09030: BWI76_RS09030 - transpeptidase, at 1,902,304 to 1,903,218
_RS09030
Position (kb)
1900
1901
1902 Strain fitness (log2 ratio)
-2
-1
0
1 at 1899.119 kb on - strand, within BWI76_RS09015 at 1899.242 kb on + strand, within BWI76_RS09015 at 1899.754 kb on + strand, within BWI76_RS09015 at 1899.772 kb on - strand, within BWI76_RS09015 at 1900.696 kb on + strand, within BWI76_RS09020 at 1900.717 kb on + strand, within BWI76_RS09020 at 1900.762 kb on + strand, within BWI76_RS09020 at 1900.762 kb on + strand, within BWI76_RS09020 at 1900.784 kb on - strand, within BWI76_RS09020 at 1900.784 kb on - strand, within BWI76_RS09020 at 1900.858 kb on + strand, within BWI76_RS09020 at 1900.953 kb on - strand, within BWI76_RS09020 at 1901.409 kb on - strand at 1901.496 kb on - strand at 1901.501 kb on - strand at 1901.700 kb on + strand, within BWI76_RS09025 at 1901.735 kb on - strand, within BWI76_RS09025 at 1902.048 kb on - strand, within BWI76_RS09025 at 1902.391 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,899,119 - BWI76_RS09015 0.21 +0.3 1,899,242 + BWI76_RS09015 0.32 -0.9 1,899,754 + BWI76_RS09015 0.82 +0.4 1,899,772 - BWI76_RS09015 0.83 +0.4 1,900,696 + BWI76_RS09020 0.44 +0.5 1,900,717 + BWI76_RS09020 0.45 -1.7 1,900,762 + BWI76_RS09020 0.48 -2.4 1,900,762 + BWI76_RS09020 0.48 +0.6 1,900,784 - BWI76_RS09020 0.50 +1.5 1,900,784 - BWI76_RS09020 0.50 +1.4 1,900,858 + BWI76_RS09020 0.55 +0.1 1,900,953 - BWI76_RS09020 0.62 +0.3 1,901,409 - -0.3 1,901,496 - -2.0 1,901,501 - -0.4 1,901,700 + BWI76_RS09025 0.22 -1.0 1,901,735 - BWI76_RS09025 0.27 +0.2 1,902,048 - BWI76_RS09025 0.70 -0.7 1,902,391 - -0.2
Or see this region's nucleotide sequence