Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08790 and BWI76_RS08795 are separated by 4 nucleotides BWI76_RS08795 and BWI76_RS08800 overlap by 4 nucleotides BWI76_RS08800 and BWI76_RS08805 are separated by 111 nucleotides
BWI76_RS08790: BWI76_RS08790 - two-component sensor histidine kinase, at 1,853,744 to 1,854,841
_RS08790
BWI76_RS08795: BWI76_RS08795 - DNA-binding response regulator, at 1,854,846 to 1,855,517
_RS08795
BWI76_RS08800: BWI76_RS08800 - phosphoethanolamine transferase EptA, at 1,855,514 to 1,857,157
_RS08800
BWI76_RS08805: BWI76_RS08805 - gluconeogenesis factor, at 1,857,269 to 1,858,174
_RS08805
Position (kb)
1855
1856
1857
1858 Strain fitness (log2 ratio)
-2
-1
0
1 at 1854.619 kb on + strand, within BWI76_RS08790 at 1854.629 kb on - strand, within BWI76_RS08790 at 1854.638 kb on + strand, within BWI76_RS08790 at 1854.821 kb on - strand at 1854.851 kb on - strand at 1854.893 kb on - strand at 1854.929 kb on + strand, within BWI76_RS08795 at 1854.937 kb on - strand, within BWI76_RS08795 at 1854.952 kb on + strand, within BWI76_RS08795 at 1855.344 kb on + strand, within BWI76_RS08795 at 1855.586 kb on - strand at 1855.839 kb on - strand, within BWI76_RS08800 at 1856.083 kb on - strand, within BWI76_RS08800 at 1856.122 kb on + strand, within BWI76_RS08800 at 1856.866 kb on + strand, within BWI76_RS08800 at 1857.043 kb on + strand at 1857.130 kb on - strand at 1857.170 kb on + strand at 1857.267 kb on + strand at 1857.274 kb on - strand at 1857.288 kb on + strand at 1857.296 kb on - strand at 1857.313 kb on - strand at 1857.347 kb on + strand at 1857.491 kb on + strand, within BWI76_RS08805 at 1857.550 kb on - strand, within BWI76_RS08805 at 1857.725 kb on + strand, within BWI76_RS08805 at 1857.978 kb on - strand, within BWI76_RS08805 at 1858.072 kb on + strand, within BWI76_RS08805
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,854,619 + BWI76_RS08790 0.80 +0.4 1,854,629 - BWI76_RS08790 0.81 +0.1 1,854,638 + BWI76_RS08790 0.81 +0.6 1,854,821 - +0.2 1,854,851 - +0.2 1,854,893 - +0.5 1,854,929 + BWI76_RS08795 0.12 +0.5 1,854,937 - BWI76_RS08795 0.14 +0.3 1,854,952 + BWI76_RS08795 0.16 -2.0 1,855,344 + BWI76_RS08795 0.74 +0.1 1,855,586 - +0.2 1,855,839 - BWI76_RS08800 0.20 +0.5 1,856,083 - BWI76_RS08800 0.35 -0.0 1,856,122 + BWI76_RS08800 0.37 +1.4 1,856,866 + BWI76_RS08800 0.82 +1.1 1,857,043 + -0.4 1,857,130 - -2.7 1,857,170 + -0.2 1,857,267 + +0.4 1,857,274 - +0.1 1,857,288 + +0.3 1,857,296 - -0.7 1,857,313 - +0.3 1,857,347 + +0.4 1,857,491 + BWI76_RS08805 0.25 +0.9 1,857,550 - BWI76_RS08805 0.31 +0.1 1,857,725 + BWI76_RS08805 0.50 +0.4 1,857,978 - BWI76_RS08805 0.78 -0.6 1,858,072 + BWI76_RS08805 0.89 +1.1
Or see this region's nucleotide sequence