Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16260 and BWI76_RS16265 are separated by 74 nucleotides BWI76_RS16265 and BWI76_RS16270 overlap by 11 nucleotides BWI76_RS16270 and BWI76_RS16275 are separated by 142 nucleotides
BWI76_RS16260: BWI76_RS16260 - two-component sensor histidine kinase, at 3,372,842 to 3,374,143
_RS16260
BWI76_RS16265: BWI76_RS16265 - DNA replication terminus site-binding protein, at 3,374,218 to 3,375,150
_RS16265
BWI76_RS16270: BWI76_RS16270 - fumarate hydratase, class II, at 3,375,140 to 3,376,540
_RS16270
BWI76_RS16275: BWI76_RS16275 - fumarate hydratase FumA, at 3,376,683 to 3,378,329
_RS16275
Position (kb)
3375
3376
3377 Strain fitness (log2 ratio)
-2
-1
0
1 at 3374.965 kb on + strand, within BWI76_RS16265 at 3375.238 kb on + strand at 3375.706 kb on + strand, within BWI76_RS16270 at 3376.030 kb on + strand, within BWI76_RS16270 at 3376.146 kb on + strand, within BWI76_RS16270 at 3376.166 kb on - strand, within BWI76_RS16270 at 3376.270 kb on + strand, within BWI76_RS16270 at 3376.367 kb on - strand, within BWI76_RS16270 at 3376.473 kb on + strand at 3376.531 kb on + strand at 3376.909 kb on - strand, within BWI76_RS16275
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,374,965 + BWI76_RS16265 0.80 -0.4 3,375,238 + +0.8 3,375,706 + BWI76_RS16270 0.40 -1.0 3,376,030 + BWI76_RS16270 0.64 +0.8 3,376,146 + BWI76_RS16270 0.72 +0.4 3,376,166 - BWI76_RS16270 0.73 +0.9 3,376,270 + BWI76_RS16270 0.81 +0.3 3,376,367 - BWI76_RS16270 0.88 +1.2 3,376,473 + -0.8 3,376,531 + +0.1 3,376,909 - BWI76_RS16275 0.14 -2.2
Or see this region's nucleotide sequence