Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13995 and BWI76_RS14000 are separated by 103 nucleotides BWI76_RS14000 and BWI76_RS14005 are separated by 2 nucleotides BWI76_RS14005 and BWI76_RS14010 are separated by 116 nucleotides
BWI76_RS13995: BWI76_RS13995 - penicillin-binding protein 2, at 2,923,874 to 2,925,793
_RS13995
BWI76_RS14000: BWI76_RS14000 - glutamine amidotransferase, at 2,925,897 to 2,926,640
_RS14000
BWI76_RS14005: BWI76_RS14005 - TetR family transcriptional regulator, at 2,926,643 to 2,927,191
_RS14005
BWI76_RS14010: BWI76_RS14010 - SDR family oxidoreductase, at 2,927,308 to 2,928,090
_RS14010
Position (kb)
2926
2927
2928 Strain fitness (log2 ratio)
-1
0
1 at 2926.067 kb on + strand, within BWI76_RS14000 at 2926.339 kb on - strand, within BWI76_RS14000 at 2926.343 kb on + strand, within BWI76_RS14000 at 2926.523 kb on - strand, within BWI76_RS14000 at 2926.570 kb on + strand at 2926.816 kb on + strand, within BWI76_RS14005 at 2927.693 kb on + strand, within BWI76_RS14010 at 2927.773 kb on + strand, within BWI76_RS14010 at 2927.810 kb on + strand, within BWI76_RS14010 at 2927.957 kb on + strand, within BWI76_RS14010 at 2928.097 kb on + strand at 2928.114 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,926,067 + BWI76_RS14000 0.23 +0.6 2,926,339 - BWI76_RS14000 0.59 +0.9 2,926,343 + BWI76_RS14000 0.60 +0.8 2,926,523 - BWI76_RS14000 0.84 -0.4 2,926,570 + +0.2 2,926,816 + BWI76_RS14005 0.32 -1.3 2,927,693 + BWI76_RS14010 0.49 +0.1 2,927,773 + BWI76_RS14010 0.59 +0.3 2,927,810 + BWI76_RS14010 0.64 +0.4 2,927,957 + BWI76_RS14010 0.83 +0.1 2,928,097 + +0.2 2,928,114 + +1.3
Or see this region's nucleotide sequence