Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12245 and BWI76_RS12250 are separated by 14 nucleotides BWI76_RS12250 and BWI76_RS12255 are separated by 4 nucleotides
BWI76_RS12245: BWI76_RS12245 - hypothetical protein, at 2,558,804 to 2,559,706
_RS12245
BWI76_RS12250: BWI76_RS12250 - terminase, at 2,559,721 to 2,560,989
_RS12250
BWI76_RS12255: BWI76_RS12255 - hypothetical protein, at 2,560,994 to 2,562,418
_RS12255
Position (kb)
2559
2560
2561 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2558.774 kb on + strand at 2558.780 kb on + strand at 2558.893 kb on - strand at 2558.898 kb on + strand, within BWI76_RS12245 at 2558.906 kb on - strand, within BWI76_RS12245 at 2558.906 kb on - strand, within BWI76_RS12245 at 2559.047 kb on + strand, within BWI76_RS12245 at 2559.060 kb on - strand, within BWI76_RS12245 at 2559.073 kb on + strand, within BWI76_RS12245 at 2559.177 kb on - strand, within BWI76_RS12245 at 2559.250 kb on + strand, within BWI76_RS12245 at 2559.356 kb on - strand, within BWI76_RS12245 at 2559.356 kb on - strand, within BWI76_RS12245 at 2559.356 kb on - strand, within BWI76_RS12245 at 2559.356 kb on - strand, within BWI76_RS12245 at 2559.508 kb on + strand, within BWI76_RS12245 at 2559.516 kb on - strand, within BWI76_RS12245 at 2559.557 kb on + strand, within BWI76_RS12245 at 2559.557 kb on + strand, within BWI76_RS12245 at 2559.634 kb on - strand at 2559.634 kb on - strand at 2559.634 kb on - strand at 2559.688 kb on - strand at 2559.823 kb on - strand at 2559.840 kb on + strand at 2559.848 kb on - strand, within BWI76_RS12250 at 2559.848 kb on - strand, within BWI76_RS12250 at 2559.983 kb on - strand, within BWI76_RS12250 at 2560.011 kb on + strand, within BWI76_RS12250 at 2560.019 kb on - strand, within BWI76_RS12250 at 2560.054 kb on + strand, within BWI76_RS12250 at 2560.175 kb on + strand, within BWI76_RS12250 at 2560.206 kb on + strand, within BWI76_RS12250 at 2560.234 kb on + strand, within BWI76_RS12250 at 2560.249 kb on + strand, within BWI76_RS12250 at 2560.314 kb on + strand, within BWI76_RS12250 at 2560.340 kb on - strand, within BWI76_RS12250 at 2560.340 kb on - strand, within BWI76_RS12250 at 2560.340 kb on - strand, within BWI76_RS12250 at 2560.384 kb on - strand, within BWI76_RS12250 at 2560.441 kb on + strand, within BWI76_RS12250 at 2560.469 kb on - strand, within BWI76_RS12250 at 2560.502 kb on + strand, within BWI76_RS12250 at 2560.510 kb on - strand, within BWI76_RS12250 at 2560.533 kb on + strand, within BWI76_RS12250 at 2560.540 kb on - strand, within BWI76_RS12250 at 2560.730 kb on + strand, within BWI76_RS12250 at 2560.776 kb on + strand, within BWI76_RS12250 at 2560.840 kb on - strand, within BWI76_RS12250 at 2561.024 kb on - strand at 2561.149 kb on + strand, within BWI76_RS12255 at 2561.171 kb on + strand, within BWI76_RS12255 at 2561.186 kb on - strand, within BWI76_RS12255 at 2561.220 kb on - strand, within BWI76_RS12255 at 2561.232 kb on - strand, within BWI76_RS12255 at 2561.237 kb on + strand, within BWI76_RS12255 at 2561.237 kb on + strand, within BWI76_RS12255 at 2561.247 kb on + strand, within BWI76_RS12255 at 2561.446 kb on + strand, within BWI76_RS12255 at 2561.446 kb on + strand, within BWI76_RS12255 at 2561.454 kb on - strand, within BWI76_RS12255 at 2561.477 kb on - strand, within BWI76_RS12255 at 2561.650 kb on + strand, within BWI76_RS12255 at 2561.717 kb on + strand, within BWI76_RS12255 at 2561.783 kb on - strand, within BWI76_RS12255 at 2561.784 kb on + strand, within BWI76_RS12255 at 2561.794 kb on + strand, within BWI76_RS12255
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,558,774 + +0.1 2,558,780 + -0.5 2,558,893 - -1.5 2,558,898 + BWI76_RS12245 0.10 -0.4 2,558,906 - BWI76_RS12245 0.11 -1.5 2,558,906 - BWI76_RS12245 0.11 -0.2 2,559,047 + BWI76_RS12245 0.27 -0.1 2,559,060 - BWI76_RS12245 0.28 -3.1 2,559,073 + BWI76_RS12245 0.30 -3.9 2,559,177 - BWI76_RS12245 0.41 +0.1 2,559,250 + BWI76_RS12245 0.49 -0.8 2,559,356 - BWI76_RS12245 0.61 -3.8 2,559,356 - BWI76_RS12245 0.61 +0.2 2,559,356 - BWI76_RS12245 0.61 -0.6 2,559,356 - BWI76_RS12245 0.61 -1.8 2,559,508 + BWI76_RS12245 0.78 -0.3 2,559,516 - BWI76_RS12245 0.79 -0.7 2,559,557 + BWI76_RS12245 0.83 -0.9 2,559,557 + BWI76_RS12245 0.83 -4.5 2,559,634 - -0.7 2,559,634 - -1.7 2,559,634 - -0.8 2,559,688 - -2.8 2,559,823 - -0.9 2,559,840 + -0.7 2,559,848 - BWI76_RS12250 0.10 -0.9 2,559,848 - BWI76_RS12250 0.10 +0.9 2,559,983 - BWI76_RS12250 0.21 -1.9 2,560,011 + BWI76_RS12250 0.23 +0.9 2,560,019 - BWI76_RS12250 0.23 -1.1 2,560,054 + BWI76_RS12250 0.26 -0.1 2,560,175 + BWI76_RS12250 0.36 -0.8 2,560,206 + BWI76_RS12250 0.38 -0.4 2,560,234 + BWI76_RS12250 0.40 -1.7 2,560,249 + BWI76_RS12250 0.42 +0.2 2,560,314 + BWI76_RS12250 0.47 -1.3 2,560,340 - BWI76_RS12250 0.49 +0.2 2,560,340 - BWI76_RS12250 0.49 -0.9 2,560,340 - BWI76_RS12250 0.49 -1.3 2,560,384 - BWI76_RS12250 0.52 -1.6 2,560,441 + BWI76_RS12250 0.57 -1.1 2,560,469 - BWI76_RS12250 0.59 -1.7 2,560,502 + BWI76_RS12250 0.62 -0.6 2,560,510 - BWI76_RS12250 0.62 -1.2 2,560,533 + BWI76_RS12250 0.64 -1.4 2,560,540 - BWI76_RS12250 0.65 -1.6 2,560,730 + BWI76_RS12250 0.80 -0.8 2,560,776 + BWI76_RS12250 0.83 -0.6 2,560,840 - BWI76_RS12250 0.88 -1.2 2,561,024 - -2.7 2,561,149 + BWI76_RS12255 0.11 -1.2 2,561,171 + BWI76_RS12255 0.12 -0.6 2,561,186 - BWI76_RS12255 0.13 -4.1 2,561,220 - BWI76_RS12255 0.16 -3.1 2,561,232 - BWI76_RS12255 0.17 -0.5 2,561,237 + BWI76_RS12255 0.17 -0.6 2,561,237 + BWI76_RS12255 0.17 -0.4 2,561,247 + BWI76_RS12255 0.18 -0.6 2,561,446 + BWI76_RS12255 0.32 -1.1 2,561,446 + BWI76_RS12255 0.32 -1.1 2,561,454 - BWI76_RS12255 0.32 -1.3 2,561,477 - BWI76_RS12255 0.34 -1.7 2,561,650 + BWI76_RS12255 0.46 -0.8 2,561,717 + BWI76_RS12255 0.51 -0.9 2,561,783 - BWI76_RS12255 0.55 -1.2 2,561,784 + BWI76_RS12255 0.55 -2.3 2,561,794 + BWI76_RS12255 0.56 -1.3
Or see this region's nucleotide sequence