Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09800 and BWI76_RS09805 are separated by 46 nucleotides BWI76_RS09805 and BWI76_RS09810 are separated by 52 nucleotides BWI76_RS09810 and BWI76_RS09815 overlap by 4 nucleotides
BWI76_RS09800: BWI76_RS09800 - tetraacyldisaccharide 4'-kinase, at 2,056,880 to 2,057,860
_RS09800
BWI76_RS09805: BWI76_RS09805 - hypothetical protein, at 2,057,907 to 2,059,136
_RS09805
BWI76_RS09810: BWI76_RS09810 - hypothetical protein, at 2,059,189 to 2,059,371
_RS09810
BWI76_RS09815: BWI76_RS09815 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 2,059,368 to 2,060,114
_RS09815
Position (kb)
2057
2058
2059
2060 Strain fitness (log2 ratio)
-1
0
1 at 2057.888 kb on + strand at 2057.897 kb on + strand at 2057.913 kb on + strand at 2057.934 kb on - strand at 2058.070 kb on + strand, within BWI76_RS09805 at 2058.108 kb on + strand, within BWI76_RS09805 at 2058.116 kb on - strand, within BWI76_RS09805 at 2058.116 kb on - strand, within BWI76_RS09805 at 2058.235 kb on - strand, within BWI76_RS09805 at 2058.853 kb on - strand, within BWI76_RS09805 at 2058.993 kb on - strand, within BWI76_RS09805
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,057,888 + +0.3 2,057,897 + +0.1 2,057,913 + +0.1 2,057,934 - +0.4 2,058,070 + BWI76_RS09805 0.13 -0.3 2,058,108 + BWI76_RS09805 0.16 +0.5 2,058,116 - BWI76_RS09805 0.17 -1.0 2,058,116 - BWI76_RS09805 0.17 -1.5 2,058,235 - BWI76_RS09805 0.27 -0.4 2,058,853 - BWI76_RS09805 0.77 -0.1 2,058,993 - BWI76_RS09805 0.88 +0.6
Or see this region's nucleotide sequence