Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08215 and BWI76_RS08220 are separated by 312 nucleotides BWI76_RS08220 and BWI76_RS08225 are separated by 178 nucleotides
BWI76_RS08215: BWI76_RS08215 - glucosamine-6-phosphate deaminase, at 1,737,921 to 1,738,721
_RS08215
BWI76_RS08220: BWI76_RS08220 - PTS N-acetyl glucosamine transporter subunit IIABC, at 1,739,034 to 1,740,986
_RS08220
BWI76_RS08225: BWI76_RS08225 - glutamine--tRNA ligase, at 1,741,165 to 1,742,832
_RS08225
Position (kb)
1739
1740
1741 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1738.242 kb on + strand, within BWI76_RS08215 at 1738.483 kb on + strand, within BWI76_RS08215 at 1738.505 kb on + strand, within BWI76_RS08215 at 1738.534 kb on + strand, within BWI76_RS08215 at 1738.696 kb on + strand at 1738.773 kb on + strand at 1739.234 kb on - strand at 1739.299 kb on + strand, within BWI76_RS08220 at 1739.595 kb on + strand, within BWI76_RS08220 at 1739.715 kb on - strand, within BWI76_RS08220 at 1739.815 kb on + strand, within BWI76_RS08220 at 1739.880 kb on - strand, within BWI76_RS08220 at 1739.932 kb on + strand, within BWI76_RS08220 at 1740.090 kb on + strand, within BWI76_RS08220 at 1740.095 kb on + strand, within BWI76_RS08220 at 1740.316 kb on - strand, within BWI76_RS08220 at 1740.823 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,738,242 + BWI76_RS08215 0.40 -3.0 1,738,483 + BWI76_RS08215 0.70 +0.8 1,738,505 + BWI76_RS08215 0.73 +0.1 1,738,534 + BWI76_RS08215 0.77 -1.8 1,738,696 + +0.4 1,738,773 + -0.2 1,739,234 - -0.3 1,739,299 + BWI76_RS08220 0.14 +0.6 1,739,595 + BWI76_RS08220 0.29 +1.0 1,739,715 - BWI76_RS08220 0.35 +1.3 1,739,815 + BWI76_RS08220 0.40 +0.7 1,739,880 - BWI76_RS08220 0.43 +0.7 1,739,932 + BWI76_RS08220 0.46 +0.4 1,740,090 + BWI76_RS08220 0.54 +1.1 1,740,095 + BWI76_RS08220 0.54 +0.3 1,740,316 - BWI76_RS08220 0.66 +0.3 1,740,823 + +0.0
Or see this region's nucleotide sequence