Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04515 and BWI76_RS04520 are separated by 2 nucleotides BWI76_RS04520 and BWI76_RS04525 overlap by 4 nucleotides BWI76_RS04525 and BWI76_RS04530 are separated by 112 nucleotides
BWI76_RS04515: BWI76_RS04515 - Co2+/Mg2+ efflux protein ApaG, at 934,244 to 934,621
_RS04515
BWI76_RS04520: BWI76_RS04520 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase, at 934,624 to 935,445
_RS04520
BWI76_RS04525: BWI76_RS04525 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 935,442 to 936,431
_RS04525
BWI76_RS04530: BWI76_RS04530 - peptidylprolyl isomerase SurA, at 936,544 to 937,830
_RS04530
Position (kb) 935
936
937 Strain fitness (log2 ratio) -2
-1
0
1 at 934.605 kb on - strand at 935.290 kb on + strand, within BWI76_RS04520 at 935.422 kb on + strand at 935.511 kb on + strand at 935.533 kb on - strand at 935.946 kb on - strand, within BWI76_RS04525 at 935.951 kb on - strand, within BWI76_RS04525 at 935.997 kb on - strand, within BWI76_RS04525 at 936.736 kb on + strand, within BWI76_RS04530 at 936.830 kb on - strand, within BWI76_RS04530 at 936.904 kb on - strand, within BWI76_RS04530 at 937.353 kb on + strand, within BWI76_RS04530
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 934,605 - -0.7 935,290 + BWI76_RS04520 0.81 -0.1 935,422 + +0.9 935,511 + +0.9 935,533 - +0.7 935,946 - BWI76_RS04525 0.51 +0.1 935,951 - BWI76_RS04525 0.51 -1.9 935,997 - BWI76_RS04525 0.56 -0.1 936,736 + BWI76_RS04530 0.15 -1.5 936,830 - BWI76_RS04530 0.22 +0.9 936,904 - BWI76_RS04530 0.28 -0.7 937,353 + BWI76_RS04530 0.63 +0.9
Or see this region's nucleotide sequence