Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02915 and BWI76_RS02920 are separated by 18 nucleotides BWI76_RS02920 and BWI76_RS02925 are separated by 25 nucleotides BWI76_RS02925 and BWI76_RS02930 are separated by 17 nucleotides BWI76_RS02930 and BWI76_RS02935 are separated by 257 nucleotides
BWI76_RS02915: BWI76_RS02915 - Hcp family T6SS protein Cts2H, at 595,729 to 596,214
_RS02915
BWI76_RS02920: BWI76_RS02920 - addiction module toxin RelE, at 596,233 to 596,379
_RS02920
BWI76_RS02925: BWI76_RS02925 - type IV secretion protein Rhs, at 596,405 to 598,123
_RS02925
BWI76_RS02930: BWI76_RS02930 - anaerobic ribonucleoside-triphosphate reductase activating protein, at 598,141 to 598,605
_RS02930
BWI76_RS02935: BWI76_RS02935 - anaerobic ribonucleoside triphosphate reductase, at 598,863 to 601,001
_RS02935
Position (kb) 596
597
598
599 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 595.444 kb on + strand at 595.444 kb on + strand at 595.444 kb on + strand at 595.444 kb on + strand at 595.444 kb on + strand at 595.452 kb on - strand at 595.452 kb on - strand at 595.500 kb on + strand at 595.508 kb on - strand at 595.508 kb on - strand at 595.545 kb on + strand at 595.545 kb on + strand at 595.545 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.554 kb on - strand at 595.554 kb on - strand at 595.554 kb on - strand at 595.598 kb on - strand at 595.612 kb on - strand at 595.623 kb on + strand at 595.623 kb on + strand at 595.623 kb on + strand at 595.623 kb on + strand at 595.623 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.690 kb on + strand at 595.698 kb on - strand at 595.700 kb on + strand at 595.700 kb on + strand at 595.700 kb on + strand at 595.700 kb on + strand at 595.700 kb on + strand at 595.700 kb on + strand at 595.708 kb on - strand at 595.708 kb on - strand at 595.718 kb on - strand at 595.720 kb on + strand at 595.720 kb on + strand at 595.722 kb on + strand at 595.765 kb on + strand at 595.765 kb on + strand at 595.774 kb on - strand at 595.776 kb on + strand at 595.784 kb on - strand, within BWI76_RS02915 at 595.814 kb on + strand at 595.817 kb on + strand, within BWI76_RS02915 at 595.817 kb on + strand, within BWI76_RS02915 at 595.817 kb on + strand, within BWI76_RS02915 at 595.817 kb on + strand, within BWI76_RS02915 at 595.817 kb on + strand, within BWI76_RS02915 at 595.825 kb on - strand, within BWI76_RS02915 at 595.825 kb on - strand, within BWI76_RS02915 at 595.825 kb on - strand, within BWI76_RS02915 at 595.851 kb on + strand, within BWI76_RS02915 at 595.851 kb on + strand, within BWI76_RS02915 at 595.869 kb on - strand, within BWI76_RS02915 at 595.932 kb on + strand, within BWI76_RS02915 at 595.939 kb on + strand, within BWI76_RS02915 at 595.941 kb on + strand, within BWI76_RS02915 at 595.947 kb on - strand, within BWI76_RS02915 at 596.048 kb on + strand, within BWI76_RS02915 at 596.215 kb on + strand at 596.216 kb on + strand at 596.223 kb on - strand at 596.231 kb on - strand at 596.239 kb on - strand at 596.332 kb on + strand, within BWI76_RS02920 at 596.427 kb on + strand at 596.638 kb on + strand, within BWI76_RS02925 at 596.803 kb on - strand, within BWI76_RS02925 at 596.895 kb on + strand, within BWI76_RS02925 at 596.972 kb on + strand, within BWI76_RS02925 at 597.121 kb on + strand, within BWI76_RS02925 at 597.213 kb on + strand, within BWI76_RS02925 at 597.253 kb on - strand, within BWI76_RS02925 at 597.280 kb on + strand, within BWI76_RS02925 at 597.280 kb on + strand, within BWI76_RS02925 at 597.534 kb on + strand, within BWI76_RS02925 at 597.544 kb on - strand, within BWI76_RS02925 at 597.717 kb on - strand, within BWI76_RS02925 at 597.744 kb on - strand, within BWI76_RS02925 at 597.770 kb on - strand, within BWI76_RS02925 at 597.921 kb on - strand, within BWI76_RS02925 at 598.153 kb on - strand at 598.281 kb on + strand, within BWI76_RS02930 at 598.521 kb on + strand, within BWI76_RS02930 at 598.552 kb on + strand, within BWI76_RS02930 at 598.559 kb on + strand at 598.591 kb on - strand at 598.853 kb on - strand at 598.924 kb on + strand at 599.019 kb on + strand at 599.027 kb on - strand at 599.118 kb on + strand, within BWI76_RS02935
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 595,444 + -2.3 595,444 + -0.6 595,444 + +0.8 595,444 + +0.6 595,444 + +0.8 595,452 - -1.7 595,452 - -1.9 595,500 + +0.9 595,508 - -0.1 595,508 - +0.1 595,545 + -1.7 595,545 + +0.9 595,545 + -2.9 595,546 + -0.4 595,546 + -1.5 595,546 + -0.4 595,546 + +0.4 595,546 + +0.6 595,546 + +0.4 595,546 + -1.1 595,554 - -0.1 595,554 - -2.8 595,554 - -0.6 595,598 - -0.5 595,612 - +0.9 595,623 + +0.3 595,623 + -0.3 595,623 + -1.1 595,623 + +0.1 595,623 + -0.1 595,680 + +0.5 595,680 + +0.2 595,680 + +0.4 595,680 + -0.9 595,680 + -0.4 595,680 + -1.0 595,680 + -0.4 595,690 + -0.6 595,698 - -0.7 595,700 + +0.3 595,700 + -0.2 595,700 + +0.4 595,700 + +0.6 595,700 + +0.7 595,700 + +0.4 595,708 - +1.6 595,708 - +0.5 595,718 - +0.5 595,720 + +0.6 595,720 + +0.2 595,722 + -1.4 595,765 + -0.5 595,765 + -1.4 595,774 - +0.9 595,776 + -0.3 595,784 - BWI76_RS02915 0.11 -1.6 595,814 + -0.1 595,817 + BWI76_RS02915 0.18 +0.9 595,817 + BWI76_RS02915 0.18 +1.5 595,817 + BWI76_RS02915 0.18 +0.4 595,817 + BWI76_RS02915 0.18 +1.5 595,817 + BWI76_RS02915 0.18 -2.3 595,825 - BWI76_RS02915 0.20 +0.2 595,825 - BWI76_RS02915 0.20 -0.5 595,825 - BWI76_RS02915 0.20 +0.4 595,851 + BWI76_RS02915 0.25 -0.1 595,851 + BWI76_RS02915 0.25 +1.6 595,869 - BWI76_RS02915 0.29 +2.4 595,932 + BWI76_RS02915 0.42 -0.3 595,939 + BWI76_RS02915 0.43 -0.0 595,941 + BWI76_RS02915 0.44 -0.4 595,947 - BWI76_RS02915 0.45 -0.1 596,048 + BWI76_RS02915 0.66 +0.9 596,215 + -0.7 596,216 + -0.4 596,223 - -0.1 596,231 - -2.3 596,239 - -0.1 596,332 + BWI76_RS02920 0.67 +0.5 596,427 + +0.6 596,638 + BWI76_RS02925 0.14 +1.3 596,803 - BWI76_RS02925 0.23 +0.5 596,895 + BWI76_RS02925 0.29 -0.5 596,972 + BWI76_RS02925 0.33 +0.6 597,121 + BWI76_RS02925 0.42 -1.2 597,213 + BWI76_RS02925 0.47 +3.2 597,253 - BWI76_RS02925 0.49 +0.5 597,280 + BWI76_RS02925 0.51 -0.2 597,280 + BWI76_RS02925 0.51 +0.6 597,534 + BWI76_RS02925 0.66 -0.3 597,544 - BWI76_RS02925 0.66 +0.3 597,717 - BWI76_RS02925 0.76 +1.5 597,744 - BWI76_RS02925 0.78 +0.4 597,770 - BWI76_RS02925 0.79 +0.2 597,921 - BWI76_RS02925 0.88 -1.1 598,153 - +0.2 598,281 + BWI76_RS02930 0.30 +0.2 598,521 + BWI76_RS02930 0.82 -0.1 598,552 + BWI76_RS02930 0.88 +0.0 598,559 + -0.9 598,591 - +0.2 598,853 - +0.4 598,924 + -1.1 599,019 + -1.6 599,027 - -0.0 599,118 + BWI76_RS02935 0.12 -0.0
Or see this region's nucleotide sequence