Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00350 and BWI76_RS00355 are separated by 396 nucleotides BWI76_RS00355 and BWI76_RS00360 are separated by 139 nucleotides BWI76_RS00360 and BWI76_RS00365 are separated by 28 nucleotides
BWI76_RS00350: BWI76_RS00350 - acyltransferase, at 72,900 to 73,799
_RS00350
BWI76_RS00355: BWI76_RS00355 - DNA polymerase I, at 74,196 to 76,985
_RS00355
BWI76_RS00360: BWI76_RS00360 - hypothetical protein, at 77,125 to 77,306
_RS00360
BWI76_RS00365: BWI76_RS00365 - YihA family ribosome biogenesis GTP-binding protein, at 77,335 to 77,967
_RS00365
Position (kb) 74
75
76
77 Strain fitness (log2 ratio) -2
-1
0
1
2 at 73.221 kb on + strand, within BWI76_RS00350 at 73.238 kb on + strand, within BWI76_RS00350 at 73.250 kb on - strand, within BWI76_RS00350 at 73.255 kb on + strand, within BWI76_RS00350 at 73.256 kb on - strand, within BWI76_RS00350 at 73.274 kb on - strand, within BWI76_RS00350 at 73.274 kb on - strand, within BWI76_RS00350 at 73.279 kb on - strand, within BWI76_RS00350 at 73.286 kb on - strand, within BWI76_RS00350 at 73.317 kb on + strand, within BWI76_RS00350 at 73.340 kb on + strand, within BWI76_RS00350 at 73.348 kb on - strand, within BWI76_RS00350 at 73.392 kb on + strand, within BWI76_RS00350 at 73.474 kb on - strand, within BWI76_RS00350 at 73.491 kb on + strand, within BWI76_RS00350 at 73.491 kb on + strand, within BWI76_RS00350 at 73.499 kb on - strand, within BWI76_RS00350 at 73.501 kb on - strand, within BWI76_RS00350 at 73.520 kb on - strand, within BWI76_RS00350 at 73.520 kb on - strand, within BWI76_RS00350 at 73.520 kb on - strand, within BWI76_RS00350 at 73.521 kb on - strand, within BWI76_RS00350 at 73.566 kb on + strand, within BWI76_RS00350 at 73.566 kb on + strand, within BWI76_RS00350 at 73.574 kb on - strand, within BWI76_RS00350 at 73.580 kb on + strand, within BWI76_RS00350 at 73.588 kb on - strand, within BWI76_RS00350 at 73.599 kb on - strand, within BWI76_RS00350 at 73.600 kb on - strand, within BWI76_RS00350 at 73.610 kb on - strand, within BWI76_RS00350 at 73.619 kb on - strand at 73.625 kb on - strand, within BWI76_RS00350 at 73.627 kb on - strand, within BWI76_RS00350 at 73.703 kb on + strand, within BWI76_RS00350 at 73.754 kb on + strand at 73.754 kb on + strand at 73.762 kb on - strand at 73.824 kb on - strand at 73.837 kb on + strand at 73.837 kb on + strand at 73.845 kb on - strand at 73.845 kb on - strand at 73.918 kb on + strand at 73.926 kb on - strand at 73.944 kb on + strand at 73.962 kb on - strand at 74.072 kb on + strand at 74.080 kb on - strand at 74.080 kb on - strand at 74.091 kb on + strand at 75.445 kb on + strand, within BWI76_RS00355 at 76.113 kb on + strand, within BWI76_RS00355 at 76.465 kb on + strand, within BWI76_RS00355 at 77.143 kb on + strand at 77.308 kb on - strand at 77.380 kb on - strand at 77.381 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 73,221 + BWI76_RS00350 0.36 -0.2 73,238 + BWI76_RS00350 0.38 +1.4 73,250 - BWI76_RS00350 0.39 -0.6 73,255 + BWI76_RS00350 0.39 +0.3 73,256 - BWI76_RS00350 0.40 +0.0 73,274 - BWI76_RS00350 0.42 -0.4 73,274 - BWI76_RS00350 0.42 +1.2 73,279 - BWI76_RS00350 0.42 +0.6 73,286 - BWI76_RS00350 0.43 -1.6 73,317 + BWI76_RS00350 0.46 -0.0 73,340 + BWI76_RS00350 0.49 -0.1 73,348 - BWI76_RS00350 0.50 +0.6 73,392 + BWI76_RS00350 0.55 -1.7 73,474 - BWI76_RS00350 0.64 +2.6 73,491 + BWI76_RS00350 0.66 -0.3 73,491 + BWI76_RS00350 0.66 -1.1 73,499 - BWI76_RS00350 0.67 +1.8 73,501 - BWI76_RS00350 0.67 -1.1 73,520 - BWI76_RS00350 0.69 -0.3 73,520 - BWI76_RS00350 0.69 -0.5 73,520 - BWI76_RS00350 0.69 +0.2 73,521 - BWI76_RS00350 0.69 +0.2 73,566 + BWI76_RS00350 0.74 -1.1 73,566 + BWI76_RS00350 0.74 +0.6 73,574 - BWI76_RS00350 0.75 -0.0 73,580 + BWI76_RS00350 0.76 -0.7 73,588 - BWI76_RS00350 0.76 +0.7 73,599 - BWI76_RS00350 0.78 +0.5 73,600 - BWI76_RS00350 0.78 +0.2 73,610 - BWI76_RS00350 0.79 -0.1 73,619 - -0.2 73,625 - BWI76_RS00350 0.81 -0.2 73,627 - BWI76_RS00350 0.81 -1.0 73,703 + BWI76_RS00350 0.89 -0.0 73,754 + +0.3 73,754 + -1.8 73,762 - -0.6 73,824 - -2.5 73,837 + +0.1 73,837 + +0.8 73,845 - +1.7 73,845 - +2.1 73,918 + +1.0 73,926 - -0.6 73,944 + -0.8 73,962 - -1.6 74,072 + +1.8 74,080 - +0.7 74,080 - +1.0 74,091 + +1.0 75,445 + BWI76_RS00355 0.45 -1.0 76,113 + BWI76_RS00355 0.69 -1.7 76,465 + BWI76_RS00355 0.81 +1.0 77,143 + +0.3 77,308 - +0.8 77,380 - +0.4 77,381 - +0.3
Or see this region's nucleotide sequence