Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26305

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26300 and BWI76_RS26305 are separated by 122 nucleotidesBWI76_RS26305 and BWI76_RS26310 are separated by 271 nucleotides BWI76_RS26300: BWI76_RS26300 - glycerol-3-phosphate transporter permease, at 5,443,117 to 5,444,004 _RS26300 BWI76_RS26305: BWI76_RS26305 - sn-glycerol-3-phosphate ABC transporter substrate-binding protein, at 5,444,127 to 5,445,443 _RS26305 BWI76_RS26310: BWI76_RS26310 - IclR family transcriptional regulator, at 5,445,715 to 5,446,482 _RS26310 Position (kb) 5444 5445 5446Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 5443.150 kb on + strandat 5443.167 kb on + strandat 5443.170 kb on - strandat 5443.554 kb on - strand, within BWI76_RS26300at 5443.666 kb on - strand, within BWI76_RS26300at 5443.782 kb on - strand, within BWI76_RS26300at 5443.837 kb on - strand, within BWI76_RS26300at 5443.886 kb on + strand, within BWI76_RS26300at 5444.072 kb on + strandat 5444.096 kb on + strandat 5444.185 kb on - strandat 5444.276 kb on + strand, within BWI76_RS26305at 5444.374 kb on - strand, within BWI76_RS26305at 5444.502 kb on - strand, within BWI76_RS26305at 5444.551 kb on + strand, within BWI76_RS26305at 5444.551 kb on + strand, within BWI76_RS26305at 5444.559 kb on - strand, within BWI76_RS26305at 5444.559 kb on - strand, within BWI76_RS26305at 5444.738 kb on + strand, within BWI76_RS26305at 5444.772 kb on + strand, within BWI76_RS26305at 5444.968 kb on - strand, within BWI76_RS26305at 5445.123 kb on + strand, within BWI76_RS26305at 5445.179 kb on + strand, within BWI76_RS26305at 5445.304 kb on - strand, within BWI76_RS26305at 5445.324 kb on - strandat 5445.370 kb on - strandat 5445.375 kb on + strandat 5445.395 kb on + strandat 5445.413 kb on + strandat 5445.421 kb on + strandat 5445.450 kb on + strandat 5445.610 kb on - strandat 5445.623 kb on - strandat 5445.623 kb on - strandat 5445.636 kb on + strandat 5445.644 kb on - strandat 5445.644 kb on - strandat 5445.698 kb on - strandat 5445.726 kb on + strandat 5445.731 kb on + strandat 5445.763 kb on + strandat 5445.771 kb on - strandat 5445.790 kb on - strandat 5445.790 kb on - strandat 5445.804 kb on + strand, within BWI76_RS26310at 5445.817 kb on - strand, within BWI76_RS26310at 5445.851 kb on - strand, within BWI76_RS26310at 5445.853 kb on + strand, within BWI76_RS26310at 5445.906 kb on + strand, within BWI76_RS26310at 5445.922 kb on + strand, within BWI76_RS26310at 5445.922 kb on + strand, within BWI76_RS26310at 5445.922 kb on + strand, within BWI76_RS26310at 5445.930 kb on - strand, within BWI76_RS26310at 5445.930 kb on - strand, within BWI76_RS26310at 5445.930 kb on - strand, within BWI76_RS26310at 5445.930 kb on - strand, within BWI76_RS26310at 5445.930 kb on - strand, within BWI76_RS26310at 5445.932 kb on + strand, within BWI76_RS26310at 5445.932 kb on + strand, within BWI76_RS26310at 5445.932 kb on + strand, within BWI76_RS26310at 5445.940 kb on - strand, within BWI76_RS26310at 5445.940 kb on - strand, within BWI76_RS26310at 5446.037 kb on + strand, within BWI76_RS26310at 5446.089 kb on + strand, within BWI76_RS26310at 5446.126 kb on + strand, within BWI76_RS26310at 5446.126 kb on + strand, within BWI76_RS26310at 5446.131 kb on + strand, within BWI76_RS26310at 5446.179 kb on - strand, within BWI76_RS26310at 5446.253 kb on - strand, within BWI76_RS26310

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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5,443,150 + +1.3
5,443,167 + -1.6
5,443,170 - +0.3
5,443,554 - BWI76_RS26300 0.49 +0.0
5,443,666 - BWI76_RS26300 0.62 -0.2
5,443,782 - BWI76_RS26300 0.75 +0.2
5,443,837 - BWI76_RS26300 0.81 -3.3
5,443,886 + BWI76_RS26300 0.87 +0.2
5,444,072 + +0.9
5,444,096 + -1.8
5,444,185 - +0.3
5,444,276 + BWI76_RS26305 0.11 +2.6
5,444,374 - BWI76_RS26305 0.19 +0.8
5,444,502 - BWI76_RS26305 0.28 -3.1
5,444,551 + BWI76_RS26305 0.32 +0.5
5,444,551 + BWI76_RS26305 0.32 -1.0
5,444,559 - BWI76_RS26305 0.33 -0.1
5,444,559 - BWI76_RS26305 0.33 -0.3
5,444,738 + BWI76_RS26305 0.46 -0.5
5,444,772 + BWI76_RS26305 0.49 -3.6
5,444,968 - BWI76_RS26305 0.64 +1.0
5,445,123 + BWI76_RS26305 0.76 -0.6
5,445,179 + BWI76_RS26305 0.80 +0.2
5,445,304 - BWI76_RS26305 0.89 -2.0
5,445,324 - +2.0
5,445,370 - -1.3
5,445,375 + +0.7
5,445,395 + -0.1
5,445,413 + -1.5
5,445,421 + +0.7
5,445,450 + +2.9
5,445,610 - +1.0
5,445,623 - +1.6
5,445,623 - -0.6
5,445,636 + +3.6
5,445,644 - +1.4
5,445,644 - +0.4
5,445,698 - +0.4
5,445,726 + +0.9
5,445,731 + -3.8
5,445,763 + +0.7
5,445,771 - +3.3
5,445,790 - +1.9
5,445,790 - +0.7
5,445,804 + BWI76_RS26310 0.12 -2.7
5,445,817 - BWI76_RS26310 0.13 -0.3
5,445,851 - BWI76_RS26310 0.18 -0.8
5,445,853 + BWI76_RS26310 0.18 -0.7
5,445,906 + BWI76_RS26310 0.25 -1.5
5,445,922 + BWI76_RS26310 0.27 +1.3
5,445,922 + BWI76_RS26310 0.27 -0.8
5,445,922 + BWI76_RS26310 0.27 -1.0
5,445,930 - BWI76_RS26310 0.28 -0.3
5,445,930 - BWI76_RS26310 0.28 -1.3
5,445,930 - BWI76_RS26310 0.28 +0.4
5,445,930 - BWI76_RS26310 0.28 -0.8
5,445,930 - BWI76_RS26310 0.28 +0.7
5,445,932 + BWI76_RS26310 0.28 -0.9
5,445,932 + BWI76_RS26310 0.28 +2.9
5,445,932 + BWI76_RS26310 0.28 +1.0
5,445,940 - BWI76_RS26310 0.29 -2.6
5,445,940 - BWI76_RS26310 0.29 +1.0
5,446,037 + BWI76_RS26310 0.42 -0.5
5,446,089 + BWI76_RS26310 0.49 -0.7
5,446,126 + BWI76_RS26310 0.54 -2.4
5,446,126 + BWI76_RS26310 0.54 +1.7
5,446,131 + BWI76_RS26310 0.54 -0.2
5,446,179 - BWI76_RS26310 0.60 +1.0
5,446,253 - BWI76_RS26310 0.70 -0.2

Or see this region's nucleotide sequence