Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13705 and BWI76_RS13710 overlap by 1 nucleotides BWI76_RS13710 and BWI76_RS13715 are separated by 9 nucleotides BWI76_RS13715 and BWI76_RS13720 are separated by 9 nucleotides BWI76_RS13720 and BWI76_RS13725 overlap by 4 nucleotides
BWI76_RS13705: BWI76_RS13705 - Rieske (2Fe-2S) domain-containing protein, at 2,859,559 to 2,860,941
_RS13705
BWI76_RS13710: BWI76_RS13710 - benzoate 1,2-dioxygenase small subunit, at 2,860,941 to 2,861,426
_RS13710
BWI76_RS13715: BWI76_RS13715 - 2-chlorobenzoate 1 2-dioxygenase electron transfer subunit, at 2,861,436 to 2,862,452
_RS13715
BWI76_RS13720: BWI76_RS13720 - 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase, at 2,862,462 to 2,863,235
_RS13720
BWI76_RS13725: BWI76_RS13725 - aromatic amino acid exporter, at 2,863,232 to 2,864,110
_RS13725
Position (kb)
2861
2862
2863 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2860.680 kb on + strand, within BWI76_RS13705 at 2860.833 kb on + strand at 2860.933 kb on - strand at 2861.043 kb on + strand, within BWI76_RS13710 at 2861.065 kb on - strand, within BWI76_RS13710 at 2861.295 kb on - strand, within BWI76_RS13710 at 2861.316 kb on - strand, within BWI76_RS13710 at 2861.740 kb on - strand, within BWI76_RS13715 at 2861.740 kb on - strand, within BWI76_RS13715 at 2861.740 kb on - strand, within BWI76_RS13715 at 2861.815 kb on - strand, within BWI76_RS13715 at 2861.902 kb on + strand, within BWI76_RS13715 at 2862.617 kb on - strand, within BWI76_RS13720 at 2862.897 kb on + strand, within BWI76_RS13720 at 2862.897 kb on + strand, within BWI76_RS13720 at 2862.949 kb on + strand at 2863.137 kb on - strand, within BWI76_RS13720 at 2863.254 kb on - strand at 2863.258 kb on + strand at 2863.322 kb on - strand, within BWI76_RS13725 at 2863.322 kb on - strand, within BWI76_RS13725 at 2863.424 kb on + strand, within BWI76_RS13725 at 2863.437 kb on + strand, within BWI76_RS13725
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,860,680 + BWI76_RS13705 0.81 +1.4 2,860,833 + +0.6 2,860,933 - -0.9 2,861,043 + BWI76_RS13710 0.21 +0.5 2,861,065 - BWI76_RS13710 0.26 +0.4 2,861,295 - BWI76_RS13710 0.73 -0.1 2,861,316 - BWI76_RS13710 0.77 +2.1 2,861,740 - BWI76_RS13715 0.30 -2.1 2,861,740 - BWI76_RS13715 0.30 -0.2 2,861,740 - BWI76_RS13715 0.30 -1.0 2,861,815 - BWI76_RS13715 0.37 +0.7 2,861,902 + BWI76_RS13715 0.46 -2.3 2,862,617 - BWI76_RS13720 0.20 +0.5 2,862,897 + BWI76_RS13720 0.56 +0.3 2,862,897 + BWI76_RS13720 0.56 +1.0 2,862,949 + -0.8 2,863,137 - BWI76_RS13720 0.87 -2.1 2,863,254 - -1.2 2,863,258 + +0.7 2,863,322 - BWI76_RS13725 0.10 -1.1 2,863,322 - BWI76_RS13725 0.10 +0.3 2,863,424 + BWI76_RS13725 0.22 -1.0 2,863,437 + BWI76_RS13725 0.23 -2.4
Or see this region's nucleotide sequence