Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11370 and BWI76_RS11375 overlap by 4 nucleotides BWI76_RS11375 and BWI76_RS11380 overlap by 4 nucleotides BWI76_RS11380 and BWI76_RS11385 are separated by 58 nucleotides
BWI76_RS11370: BWI76_RS11370 - iron-dicitrate transporter subunit FecD, at 2,396,149 to 2,397,102
_RS11370
BWI76_RS11375: BWI76_RS11375 - iron-dicitrate transporter permease subunit, at 2,397,099 to 2,398,097
_RS11375
BWI76_RS11380: BWI76_RS11380 - iron-dicitrate transporter substrate-binding subunit, at 2,398,094 to 2,398,996
_RS11380
BWI76_RS11385: BWI76_RS11385 - TonB-dependent siderophore receptor, at 2,399,055 to 2,401,385
_RS11385
Position (kb)
2398
2399 Strain fitness (log2 ratio)
-1
0
1
2 at 2397.136 kb on + strand at 2397.144 kb on - strand at 2397.183 kb on + strand at 2397.191 kb on - strand at 2397.264 kb on + strand, within BWI76_RS11375 at 2397.605 kb on - strand, within BWI76_RS11375 at 2397.760 kb on + strand, within BWI76_RS11375 at 2398.281 kb on + strand, within BWI76_RS11380 at 2398.328 kb on - strand, within BWI76_RS11380 at 2398.416 kb on - strand, within BWI76_RS11380 at 2398.872 kb on - strand, within BWI76_RS11380 at 2398.883 kb on - strand, within BWI76_RS11380 at 2398.908 kb on - strand at 2398.958 kb on - strand at 2399.078 kb on + strand at 2399.208 kb on + strand at 2399.789 kb on - strand, within BWI76_RS11385 at 2399.789 kb on - strand, within BWI76_RS11385 at 2399.853 kb on + strand, within BWI76_RS11385 at 2399.885 kb on + strand, within BWI76_RS11385
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,397,136 + +0.2 2,397,144 - -0.3 2,397,183 + +0.3 2,397,191 - -1.5 2,397,264 + BWI76_RS11375 0.17 +0.2 2,397,605 - BWI76_RS11375 0.51 +0.2 2,397,760 + BWI76_RS11375 0.66 +0.7 2,398,281 + BWI76_RS11380 0.21 +0.7 2,398,328 - BWI76_RS11380 0.26 +0.5 2,398,416 - BWI76_RS11380 0.36 +1.0 2,398,872 - BWI76_RS11380 0.86 +0.8 2,398,883 - BWI76_RS11380 0.87 +1.0 2,398,908 - -0.2 2,398,958 - +0.6 2,399,078 + -0.2 2,399,208 + +1.4 2,399,789 - BWI76_RS11385 0.31 -0.0 2,399,789 - BWI76_RS11385 0.31 +1.0 2,399,853 + BWI76_RS11385 0.34 +1.8 2,399,885 + BWI76_RS11385 0.36 +0.6
Or see this region's nucleotide sequence