Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08925 and BWI76_RS08930 are separated by 122 nucleotides BWI76_RS08930 and BWI76_RS08935 are separated by 285 nucleotides BWI76_RS08935 and BWI76_RS08940 are separated by 68 nucleotides
BWI76_RS08925: BWI76_RS08925 - DNA-binding protein YbiB, at 1,880,663 to 1,881,631
_RS08925
BWI76_RS08930: BWI76_RS08930 - hypothetical protein, at 1,881,754 to 1,882,014
_RS08930
BWI76_RS08935: BWI76_RS08935 - hypothetical protein, at 1,882,300 to 1,882,566
_RS08935
BWI76_RS08940: BWI76_RS08940 - PKHD-type hydroxylase, at 1,882,635 to 1,883,312
_RS08940
Position (kb)
1881
1882
1883 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1880.773 kb on + strand, within BWI76_RS08925 at 1880.799 kb on + strand, within BWI76_RS08925 at 1881.086 kb on + strand, within BWI76_RS08925 at 1881.201 kb on - strand, within BWI76_RS08925 at 1881.368 kb on + strand, within BWI76_RS08925 at 1881.510 kb on + strand, within BWI76_RS08925 at 1881.706 kb on + strand at 1881.727 kb on - strand at 1881.768 kb on + strand at 1882.032 kb on - strand at 1882.034 kb on + strand at 1882.034 kb on + strand at 1882.211 kb on + strand at 1882.571 kb on - strand at 1882.576 kb on + strand at 1882.589 kb on + strand at 1882.591 kb on + strand at 1882.661 kb on + strand at 1882.839 kb on + strand, within BWI76_RS08940 at 1882.898 kb on + strand, within BWI76_RS08940
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,880,773 + BWI76_RS08925 0.11 -0.6 1,880,799 + BWI76_RS08925 0.14 -3.6 1,881,086 + BWI76_RS08925 0.44 -1.8 1,881,201 - BWI76_RS08925 0.56 +1.3 1,881,368 + BWI76_RS08925 0.73 +0.8 1,881,510 + BWI76_RS08925 0.87 +1.0 1,881,706 + +0.5 1,881,727 - +0.3 1,881,768 + -2.2 1,882,032 - -0.2 1,882,034 + -2.0 1,882,034 + -4.2 1,882,211 + -0.0 1,882,571 - -0.2 1,882,576 + +0.5 1,882,589 + +1.6 1,882,591 + +1.6 1,882,661 + +0.9 1,882,839 + BWI76_RS08940 0.30 -0.4 1,882,898 + BWI76_RS08940 0.39 +0.6
Or see this region's nucleotide sequence