Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05425 and BWI76_RS05430 are separated by 84 nucleotides BWI76_RS05430 and BWI76_RS05435 are separated by 57 nucleotides BWI76_RS05435 and BWI76_RS05440 are separated by 71 nucleotides BWI76_RS05440 and BWI76_RS05445 are separated by 32 nucleotides
BWI76_RS05425: BWI76_RS05425 - EEP domain-containing protein, at 1,139,297 to 1,140,103
_RS05425
BWI76_RS05430: BWI76_RS05430 - UbiE/COQ5 family methyltransferase, at 1,140,188 to 1,140,958
_RS05430
BWI76_RS05435: BWI76_RS05435 - murein transglycosylase D, at 1,141,016 to 1,142,383
_RS05435
BWI76_RS05440: BWI76_RS05440 - hydroxyacylglutathione hydrolase, at 1,142,455 to 1,143,210
_RS05440
BWI76_RS05445: BWI76_RS05445 - type 11 methyltransferase, at 1,143,243 to 1,143,965
_RS05445
Position (kb)
1141
1142
1143 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1140.133 kb on + strand at 1140.211 kb on - strand at 1140.243 kb on + strand at 1140.251 kb on - strand at 1140.256 kb on - strand at 1140.481 kb on + strand, within BWI76_RS05430 at 1140.540 kb on + strand, within BWI76_RS05430 at 1140.752 kb on - strand, within BWI76_RS05430 at 1141.076 kb on - strand at 1141.147 kb on - strand at 1141.154 kb on + strand, within BWI76_RS05435 at 1141.358 kb on + strand, within BWI76_RS05435 at 1141.378 kb on - strand, within BWI76_RS05435 at 1141.415 kb on + strand, within BWI76_RS05435 at 1141.503 kb on - strand, within BWI76_RS05435 at 1141.592 kb on + strand, within BWI76_RS05435 at 1141.632 kb on - strand, within BWI76_RS05435 at 1141.795 kb on - strand, within BWI76_RS05435 at 1141.987 kb on + strand, within BWI76_RS05435 at 1141.995 kb on - strand, within BWI76_RS05435 at 1142.204 kb on - strand, within BWI76_RS05435 at 1142.208 kb on - strand, within BWI76_RS05435 at 1142.230 kb on + strand, within BWI76_RS05435 at 1142.230 kb on + strand, within BWI76_RS05435 at 1142.278 kb on - strand at 1142.305 kb on + strand at 1142.384 kb on - strand at 1142.384 kb on - strand at 1142.453 kb on - strand at 1142.469 kb on + strand at 1142.491 kb on + strand at 1142.491 kb on + strand at 1142.499 kb on - strand at 1142.499 kb on - strand at 1142.637 kb on - strand, within BWI76_RS05440 at 1142.692 kb on + strand, within BWI76_RS05440 at 1142.715 kb on + strand, within BWI76_RS05440 at 1142.718 kb on - strand, within BWI76_RS05440 at 1142.736 kb on - strand, within BWI76_RS05440 at 1142.750 kb on + strand, within BWI76_RS05440 at 1142.800 kb on - strand, within BWI76_RS05440 at 1142.800 kb on - strand, within BWI76_RS05440 at 1142.800 kb on - strand, within BWI76_RS05440 at 1142.841 kb on + strand, within BWI76_RS05440 at 1142.879 kb on - strand, within BWI76_RS05440 at 1142.879 kb on - strand, within BWI76_RS05440 at 1143.029 kb on + strand, within BWI76_RS05440 at 1143.076 kb on - strand, within BWI76_RS05440 at 1143.264 kb on - strand at 1143.294 kb on + strand at 1143.352 kb on - strand, within BWI76_RS05445 at 1143.352 kb on - strand, within BWI76_RS05445
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,140,133 + -0.3 1,140,211 - -0.2 1,140,243 + +0.3 1,140,251 - -0.0 1,140,256 - +0.6 1,140,481 + BWI76_RS05430 0.38 +0.8 1,140,540 + BWI76_RS05430 0.46 +1.0 1,140,752 - BWI76_RS05430 0.73 +0.2 1,141,076 - -0.7 1,141,147 - +0.1 1,141,154 + BWI76_RS05435 0.10 +0.4 1,141,358 + BWI76_RS05435 0.25 -3.1 1,141,378 - BWI76_RS05435 0.26 +0.7 1,141,415 + BWI76_RS05435 0.29 +1.0 1,141,503 - BWI76_RS05435 0.36 -0.0 1,141,592 + BWI76_RS05435 0.42 +0.1 1,141,632 - BWI76_RS05435 0.45 +1.0 1,141,795 - BWI76_RS05435 0.57 +1.0 1,141,987 + BWI76_RS05435 0.71 +0.9 1,141,995 - BWI76_RS05435 0.72 +0.5 1,142,204 - BWI76_RS05435 0.87 +0.6 1,142,208 - BWI76_RS05435 0.87 +0.9 1,142,230 + BWI76_RS05435 0.89 +0.4 1,142,230 + BWI76_RS05435 0.89 +0.3 1,142,278 - +0.4 1,142,305 + +1.0 1,142,384 - -1.1 1,142,384 - -0.3 1,142,453 - +0.1 1,142,469 + -1.7 1,142,491 + -1.7 1,142,491 + -1.0 1,142,499 - -1.1 1,142,499 - -0.0 1,142,637 - BWI76_RS05440 0.24 -3.5 1,142,692 + BWI76_RS05440 0.31 -1.7 1,142,715 + BWI76_RS05440 0.34 +0.8 1,142,718 - BWI76_RS05440 0.35 -0.1 1,142,736 - BWI76_RS05440 0.37 -0.2 1,142,750 + BWI76_RS05440 0.39 -2.4 1,142,800 - BWI76_RS05440 0.46 -3.1 1,142,800 - BWI76_RS05440 0.46 -0.2 1,142,800 - BWI76_RS05440 0.46 +1.3 1,142,841 + BWI76_RS05440 0.51 -1.1 1,142,879 - BWI76_RS05440 0.56 -2.3 1,142,879 - BWI76_RS05440 0.56 -1.2 1,143,029 + BWI76_RS05440 0.76 -0.8 1,143,076 - BWI76_RS05440 0.82 +0.6 1,143,264 - -3.5 1,143,294 + +0.6 1,143,352 - BWI76_RS05445 0.15 -0.2 1,143,352 - BWI76_RS05445 0.15 -1.0
Or see this region's nucleotide sequence