Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05420 and BWI76_RS05425 are separated by 94 nucleotides BWI76_RS05425 and BWI76_RS05430 are separated by 84 nucleotides BWI76_RS05430 and BWI76_RS05435 are separated by 57 nucleotides
BWI76_RS05420: BWI76_RS05420 - MFS transporter, at 1,138,012 to 1,139,202
_RS05420
BWI76_RS05425: BWI76_RS05425 - EEP domain-containing protein, at 1,139,297 to 1,140,103
_RS05425
BWI76_RS05430: BWI76_RS05430 - UbiE/COQ5 family methyltransferase, at 1,140,188 to 1,140,958
_RS05430
BWI76_RS05435: BWI76_RS05435 - murein transglycosylase D, at 1,141,016 to 1,142,383
_RS05435
Position (kb)
1140
1141 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1139.205 kb on - strand at 1139.242 kb on + strand at 1139.367 kb on - strand at 1139.456 kb on + strand, within BWI76_RS05425 at 1139.801 kb on - strand, within BWI76_RS05425 at 1139.890 kb on - strand, within BWI76_RS05425 at 1140.133 kb on + strand at 1140.211 kb on - strand at 1140.243 kb on + strand at 1140.251 kb on - strand at 1140.256 kb on - strand at 1140.481 kb on + strand, within BWI76_RS05430 at 1140.540 kb on + strand, within BWI76_RS05430 at 1140.752 kb on - strand, within BWI76_RS05430 at 1141.076 kb on - strand at 1141.147 kb on - strand at 1141.154 kb on + strand, within BWI76_RS05435 at 1141.358 kb on + strand, within BWI76_RS05435 at 1141.378 kb on - strand, within BWI76_RS05435 at 1141.415 kb on + strand, within BWI76_RS05435 at 1141.503 kb on - strand, within BWI76_RS05435 at 1141.592 kb on + strand, within BWI76_RS05435 at 1141.632 kb on - strand, within BWI76_RS05435 at 1141.795 kb on - strand, within BWI76_RS05435
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,139,205 - -1.4 1,139,242 + +0.6 1,139,367 - +0.3 1,139,456 + BWI76_RS05425 0.20 -0.5 1,139,801 - BWI76_RS05425 0.62 +0.3 1,139,890 - BWI76_RS05425 0.73 +0.5 1,140,133 + -0.3 1,140,211 - -0.2 1,140,243 + +0.3 1,140,251 - -0.0 1,140,256 - +0.6 1,140,481 + BWI76_RS05430 0.38 +0.8 1,140,540 + BWI76_RS05430 0.46 +1.0 1,140,752 - BWI76_RS05430 0.73 +0.2 1,141,076 - -0.7 1,141,147 - +0.1 1,141,154 + BWI76_RS05435 0.10 +0.4 1,141,358 + BWI76_RS05435 0.25 -3.1 1,141,378 - BWI76_RS05435 0.26 +0.7 1,141,415 + BWI76_RS05435 0.29 +1.0 1,141,503 - BWI76_RS05435 0.36 -0.0 1,141,592 + BWI76_RS05435 0.42 +0.1 1,141,632 - BWI76_RS05435 0.45 +1.0 1,141,795 - BWI76_RS05435 0.57 +1.0
Or see this region's nucleotide sequence