Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04485 and BWI76_RS04490 are separated by 166 nucleotides BWI76_RS04490 and BWI76_RS04495 overlap by 8 nucleotides
BWI76_RS04485: BWI76_RS04485 - hypothetical protein, at 928,559 to 930,115
_RS04485
BWI76_RS04490: BWI76_RS04490 - glutathione-regulated potassium-efflux system ancillary protein KefF, at 930,282 to 930,815
_RS04490
BWI76_RS04495: BWI76_RS04495 - glutathione-regulated potassium-efflux system protein KefC, at 930,808 to 932,673
_RS04495
Position (kb) 930
931 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 929.284 kb on + strand, within BWI76_RS04485 at 929.284 kb on + strand, within BWI76_RS04485 at 929.284 kb on + strand, within BWI76_RS04485 at 929.284 kb on + strand, within BWI76_RS04485 at 929.284 kb on + strand at 929.287 kb on + strand, within BWI76_RS04485 at 929.307 kb on - strand, within BWI76_RS04485 at 929.328 kb on + strand, within BWI76_RS04485 at 929.329 kb on - strand, within BWI76_RS04485 at 929.333 kb on + strand, within BWI76_RS04485 at 929.333 kb on + strand, within BWI76_RS04485 at 929.333 kb on + strand, within BWI76_RS04485 at 929.333 kb on + strand, within BWI76_RS04485 at 929.333 kb on + strand, within BWI76_RS04485 at 929.336 kb on - strand, within BWI76_RS04485 at 929.339 kb on + strand, within BWI76_RS04485 at 929.339 kb on + strand, within BWI76_RS04485 at 929.339 kb on + strand, within BWI76_RS04485 at 929.339 kb on + strand, within BWI76_RS04485 at 929.339 kb on + strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand at 929.341 kb on - strand, within BWI76_RS04485 at 929.342 kb on - strand at 929.342 kb on - strand, within BWI76_RS04485 at 929.342 kb on - strand, within BWI76_RS04485 at 929.342 kb on - strand, within BWI76_RS04485 at 929.346 kb on + strand, within BWI76_RS04485 at 929.347 kb on - strand at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.354 kb on - strand, within BWI76_RS04485 at 929.354 kb on - strand, within BWI76_RS04485 at 929.354 kb on - strand, within BWI76_RS04485 at 929.389 kb on + strand, within BWI76_RS04485 at 929.405 kb on - strand, within BWI76_RS04485 at 929.417 kb on + strand, within BWI76_RS04485 at 929.562 kb on + strand, within BWI76_RS04485 at 929.858 kb on - strand, within BWI76_RS04485 at 929.867 kb on - strand, within BWI76_RS04485 at 929.872 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.958 kb on + strand, within BWI76_RS04485 at 929.958 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 930.037 kb on - strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.041 kb on + strand at 930.047 kb on - strand at 930.074 kb on + strand at 930.074 kb on + strand at 930.082 kb on - strand at 930.093 kb on - strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.114 kb on + strand at 930.117 kb on - strand at 930.117 kb on - strand at 930.117 kb on - strand at 930.117 kb on - strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.127 kb on - strand at 930.135 kb on - strand at 930.139 kb on + strand at 930.147 kb on - strand at 930.147 kb on - strand at 930.159 kb on + strand at 930.166 kb on + strand at 930.166 kb on + strand at 930.174 kb on + strand at 930.174 kb on + strand at 930.174 kb on + strand at 930.174 kb on + strand at 930.174 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.184 kb on + strand at 930.184 kb on + strand at 930.184 kb on + strand at 930.185 kb on + strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.215 kb on - strand at 930.243 kb on + strand at 930.244 kb on - strand at 930.245 kb on - strand at 930.251 kb on - strand at 930.253 kb on - strand at 930.259 kb on - strand at 930.263 kb on + strand at 930.264 kb on - strand at 930.264 kb on - strand at 930.270 kb on - strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.313 kb on - strand at 930.313 kb on - strand at 930.313 kb on - strand at 930.334 kb on + strand at 930.340 kb on + strand, within BWI76_RS04490 at 930.340 kb on + strand, within BWI76_RS04490 at 930.350 kb on + strand, within BWI76_RS04490 at 930.358 kb on - strand, within BWI76_RS04490 at 930.367 kb on + strand, within BWI76_RS04490 at 930.367 kb on + strand, within BWI76_RS04490 at 930.369 kb on + strand, within BWI76_RS04490 at 930.380 kb on - strand, within BWI76_RS04490 at 930.421 kb on + strand, within BWI76_RS04490 at 930.435 kb on + strand, within BWI76_RS04490 at 930.435 kb on + strand, within BWI76_RS04490 at 930.435 kb on + strand, within BWI76_RS04490 at 930.443 kb on - strand, within BWI76_RS04490 at 930.445 kb on + strand, within BWI76_RS04490 at 930.485 kb on - strand, within BWI76_RS04490 at 930.495 kb on - strand, within BWI76_RS04490 at 930.523 kb on - strand, within BWI76_RS04490 at 930.528 kb on + strand, within BWI76_RS04490 at 930.533 kb on + strand, within BWI76_RS04490 at 930.680 kb on + strand, within BWI76_RS04490 at 930.815 kb on + strand at 930.815 kb on + strand at 930.881 kb on + strand at 930.889 kb on - strand at 930.997 kb on + strand, within BWI76_RS04495 at 931.027 kb on - strand, within BWI76_RS04495 at 931.042 kb on - strand, within BWI76_RS04495 at 931.116 kb on + strand, within BWI76_RS04495 at 931.169 kb on + strand, within BWI76_RS04495 at 931.179 kb on + strand, within BWI76_RS04495 at 931.187 kb on - strand, within BWI76_RS04495 at 931.250 kb on + strand, within BWI76_RS04495 at 931.795 kb on - strand, within BWI76_RS04495
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 929,284 + BWI76_RS04485 0.47 +2.1 929,284 + BWI76_RS04485 0.47 -1.9 929,284 + BWI76_RS04485 0.47 +0.8 929,284 + BWI76_RS04485 0.47 +0.3 929,284 + +2.5 929,287 + BWI76_RS04485 0.47 +0.9 929,307 - BWI76_RS04485 0.48 -2.4 929,328 + BWI76_RS04485 0.49 -0.3 929,329 - BWI76_RS04485 0.49 -1.9 929,333 + BWI76_RS04485 0.50 +1.2 929,333 + BWI76_RS04485 0.50 -1.7 929,333 + BWI76_RS04485 0.50 -1.9 929,333 + BWI76_RS04485 0.50 +0.9 929,333 + BWI76_RS04485 0.50 -0.1 929,336 - BWI76_RS04485 0.50 -3.6 929,339 + BWI76_RS04485 0.50 +1.9 929,339 + BWI76_RS04485 0.50 -2.1 929,339 + BWI76_RS04485 0.50 +0.9 929,339 + BWI76_RS04485 0.50 -0.5 929,339 + BWI76_RS04485 0.50 -1.4 929,341 - BWI76_RS04485 0.50 -2.1 929,341 - BWI76_RS04485 0.50 -1.7 929,341 - BWI76_RS04485 0.50 -2.7 929,341 - BWI76_RS04485 0.50 -0.7 929,341 - BWI76_RS04485 0.50 +0.9 929,341 - BWI76_RS04485 0.50 +1.4 929,341 - BWI76_RS04485 0.50 +0.4 929,341 - BWI76_RS04485 0.50 +0.9 929,341 - BWI76_RS04485 0.50 -1.4 929,341 - +0.9 929,341 - BWI76_RS04485 0.50 +2.2 929,342 - -0.7 929,342 - BWI76_RS04485 0.50 -2.3 929,342 - BWI76_RS04485 0.50 +0.7 929,342 - BWI76_RS04485 0.50 -3.0 929,346 + BWI76_RS04485 0.51 +0.5 929,347 - +2.2 929,347 - BWI76_RS04485 0.51 -1.0 929,347 - BWI76_RS04485 0.51 +0.9 929,347 - BWI76_RS04485 0.51 -0.7 929,347 - BWI76_RS04485 0.51 -1.7 929,347 - BWI76_RS04485 0.51 -1.7 929,347 - BWI76_RS04485 0.51 -0.1 929,347 - BWI76_RS04485 0.51 -0.1 929,347 - BWI76_RS04485 0.51 -2.8 929,347 - BWI76_RS04485 0.51 -1.7 929,354 - BWI76_RS04485 0.51 +1.5 929,354 - BWI76_RS04485 0.51 +3.0 929,354 - BWI76_RS04485 0.51 +0.9 929,389 + BWI76_RS04485 0.53 +1.4 929,405 - BWI76_RS04485 0.54 -0.7 929,417 + BWI76_RS04485 0.55 -0.7 929,562 + BWI76_RS04485 0.64 -0.1 929,858 - BWI76_RS04485 0.83 -0.0 929,867 - BWI76_RS04485 0.84 -0.3 929,872 + BWI76_RS04485 0.84 -1.1 929,904 + BWI76_RS04485 0.86 -1.7 929,904 + BWI76_RS04485 0.86 -2.7 929,904 + BWI76_RS04485 0.86 -1.9 929,904 + BWI76_RS04485 0.86 -1.1 929,904 + BWI76_RS04485 0.86 -2.2 929,904 + BWI76_RS04485 0.86 -0.3 929,904 + BWI76_RS04485 0.86 +0.9 929,912 - BWI76_RS04485 0.87 -1.1 929,912 - BWI76_RS04485 0.87 +0.1 929,912 - BWI76_RS04485 0.87 -1.7 929,912 - BWI76_RS04485 0.87 -1.4 929,912 - BWI76_RS04485 0.87 -1.4 929,912 - BWI76_RS04485 0.87 +1.1 929,958 + BWI76_RS04485 0.90 -1.4 929,958 + BWI76_RS04485 0.90 -3.2 929,959 + BWI76_RS04485 0.90 +2.9 929,959 + BWI76_RS04485 0.90 -2.0 929,959 + BWI76_RS04485 0.90 +0.2 929,959 + BWI76_RS04485 0.90 -0.3 929,959 + BWI76_RS04485 0.90 -0.5 929,967 - -0.2 929,967 - -1.4 929,967 - +0.9 929,967 - +0.9 929,967 - -2.7 929,967 - +0.9 929,967 - -1.2 930,037 - -1.1 930,039 + +1.2 930,039 + -2.8 930,039 + -1.1 930,039 + -0.8 930,039 + -0.5 930,039 + -1.7 930,039 + +0.9 930,039 + -2.7 930,039 + +1.6 930,041 + +0.7 930,047 - +0.9 930,074 + -0.3 930,074 + -2.7 930,082 - +0.2 930,093 - -2.2 930,109 + +1.5 930,109 + -0.5 930,109 + -0.4 930,109 + -1.1 930,109 + -0.7 930,109 + -0.1 930,109 + -2.1 930,109 + +0.4 930,114 + -0.1 930,117 - -2.4 930,117 - +0.8 930,117 - -3.0 930,117 - +2.5 930,119 + -1.4 930,119 + -0.2 930,119 + -0.2 930,119 + -0.5 930,119 + +0.8 930,127 - +0.9 930,135 - +1.9 930,139 + +0.4 930,147 - -0.7 930,147 - -1.4 930,159 + -0.8 930,166 + +0.9 930,166 + +1.5 930,174 + +0.9 930,174 + +2.1 930,174 + +1.3 930,174 + +1.2 930,174 - +0.0 930,182 - -0.7 930,182 - +0.6 930,182 - -0.1 930,182 - +1.2 930,182 - +0.3 930,182 - +2.0 930,182 - -0.1 930,182 - -3.1 930,182 - +0.4 930,182 - +1.3 930,182 - -0.0 930,182 - -0.4 930,182 - -1.7 930,182 - +0.3 930,184 + +0.4 930,184 + +0.7 930,184 + -3.2 930,185 + -3.1 930,192 - +0.9 930,192 - +0.3 930,192 - -3.8 930,192 - +0.3 930,192 - +0.2 930,192 - +0.5 930,215 - -0.1 930,243 + -1.9 930,244 - +0.5 930,245 - +0.3 930,251 - +0.7 930,253 - -0.1 930,259 - +1.0 930,263 + +0.0 930,264 - -1.3 930,264 - +1.2 930,270 - +0.2 930,305 + +0.2 930,305 + -0.1 930,305 + -0.5 930,305 + -1.2 930,305 + +0.5 930,313 - -2.9 930,313 - -0.9 930,313 - +0.4 930,334 + +0.8 930,340 + BWI76_RS04490 0.11 +0.7 930,340 + BWI76_RS04490 0.11 -1.9 930,350 + BWI76_RS04490 0.13 +0.0 930,358 - BWI76_RS04490 0.14 -3.7 930,367 + BWI76_RS04490 0.16 -1.7 930,367 + BWI76_RS04490 0.16 -0.1 930,369 + BWI76_RS04490 0.16 -0.3 930,380 - BWI76_RS04490 0.18 +0.1 930,421 + BWI76_RS04490 0.26 -1.8 930,435 + BWI76_RS04490 0.29 +0.2 930,435 + BWI76_RS04490 0.29 -0.1 930,435 + BWI76_RS04490 0.29 -0.2 930,443 - BWI76_RS04490 0.30 -1.1 930,445 + BWI76_RS04490 0.31 -0.2 930,485 - BWI76_RS04490 0.38 +0.5 930,495 - BWI76_RS04490 0.40 -0.7 930,523 - BWI76_RS04490 0.45 -1.2 930,528 + BWI76_RS04490 0.46 -0.6 930,533 + BWI76_RS04490 0.47 +0.6 930,680 + BWI76_RS04490 0.75 -1.1 930,815 + -2.5 930,815 + -1.4 930,881 + +0.7 930,889 - -0.3 930,997 + BWI76_RS04495 0.10 +0.7 931,027 - BWI76_RS04495 0.12 +1.0 931,042 - BWI76_RS04495 0.13 -0.2 931,116 + BWI76_RS04495 0.17 +1.3 931,169 + BWI76_RS04495 0.19 +0.5 931,179 + BWI76_RS04495 0.20 -0.9 931,187 - BWI76_RS04495 0.20 +0.9 931,250 + BWI76_RS04495 0.24 +0.6 931,795 - BWI76_RS04495 0.53 -2.8
Or see this region's nucleotide sequence