Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19180

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19175 and BWI76_RS19180 are separated by 234 nucleotidesBWI76_RS19180 and BWI76_RS19185 overlap by 20 nucleotides BWI76_RS19175: BWI76_RS19175 - dicarboxylate/amino acid:cation symporter, at 3,990,445 to 3,991,719 _RS19175 BWI76_RS19180: BWI76_RS19180 - diguanylate cyclase, at 3,991,954 to 3,993,468 _RS19180 BWI76_RS19185: BWI76_RS19185 - ABC transporter ATP-binding protein, at 3,993,449 to 3,994,483 _RS19185 Position (kb) 3991 3992 3993 3994Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3990.978 kb on + strand, within BWI76_RS19175at 3990.986 kb on - strand, within BWI76_RS19175at 3991.052 kb on + strand, within BWI76_RS19175at 3991.254 kb on - strand, within BWI76_RS19175at 3991.401 kb on + strand, within BWI76_RS19175at 3991.409 kb on - strand, within BWI76_RS19175at 3991.420 kb on - strand, within BWI76_RS19175at 3991.542 kb on - strand, within BWI76_RS19175at 3991.542 kb on - strand, within BWI76_RS19175at 3991.642 kb on - strandat 3991.642 kb on - strandat 3991.664 kb on + strandat 3991.668 kb on - strandat 3991.674 kb on - strandat 3991.685 kb on + strandat 3992.030 kb on + strandat 3992.045 kb on + strandat 3992.069 kb on + strandat 3992.099 kb on + strandat 3992.099 kb on + strandat 3992.107 kb on - strand, within BWI76_RS19180at 3992.107 kb on - strand, within BWI76_RS19180at 3992.164 kb on - strand, within BWI76_RS19180at 3992.263 kb on - strand, within BWI76_RS19180at 3992.263 kb on - strand, within BWI76_RS19180at 3992.348 kb on + strand, within BWI76_RS19180at 3992.414 kb on + strand, within BWI76_RS19180at 3992.459 kb on + strand, within BWI76_RS19180at 3992.599 kb on + strand, within BWI76_RS19180at 3992.624 kb on - strand, within BWI76_RS19180at 3992.758 kb on - strand, within BWI76_RS19180at 3993.039 kb on + strand, within BWI76_RS19180at 3993.240 kb on - strand, within BWI76_RS19180at 3993.296 kb on - strand, within BWI76_RS19180at 3993.597 kb on + strand, within BWI76_RS19185at 3993.731 kb on - strand, within BWI76_RS19185at 3993.743 kb on + strand, within BWI76_RS19185at 3993.890 kb on + strand, within BWI76_RS19185at 3993.915 kb on - strand, within BWI76_RS19185at 3994.031 kb on + strand, within BWI76_RS19185at 3994.236 kb on + strand, within BWI76_RS19185at 3994.294 kb on + strand, within BWI76_RS19185at 3994.373 kb on + strand, within BWI76_RS19185at 3994.438 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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3,990,978 + BWI76_RS19175 0.42 -4.4
3,990,986 - BWI76_RS19175 0.42 +0.4
3,991,052 + BWI76_RS19175 0.48 +1.0
3,991,254 - BWI76_RS19175 0.63 +0.4
3,991,401 + BWI76_RS19175 0.75 +0.8
3,991,409 - BWI76_RS19175 0.76 -0.2
3,991,420 - BWI76_RS19175 0.76 +0.0
3,991,542 - BWI76_RS19175 0.86 +0.0
3,991,542 - BWI76_RS19175 0.86 +0.3
3,991,642 - +0.1
3,991,642 - +1.2
3,991,664 + +0.1
3,991,668 - -1.8
3,991,674 - -1.5
3,991,685 + -0.4
3,992,030 + +0.2
3,992,045 + -0.8
3,992,069 + +0.6
3,992,099 + +1.0
3,992,099 + -0.3
3,992,107 - BWI76_RS19180 0.10 +0.4
3,992,107 - BWI76_RS19180 0.10 -1.3
3,992,164 - BWI76_RS19180 0.14 -0.3
3,992,263 - BWI76_RS19180 0.20 +1.5
3,992,263 - BWI76_RS19180 0.20 +0.6
3,992,348 + BWI76_RS19180 0.26 -0.3
3,992,414 + BWI76_RS19180 0.30 +0.7
3,992,459 + BWI76_RS19180 0.33 +1.0
3,992,599 + BWI76_RS19180 0.43 +0.8
3,992,624 - BWI76_RS19180 0.44 +0.3
3,992,758 - BWI76_RS19180 0.53 -0.0
3,993,039 + BWI76_RS19180 0.72 +0.1
3,993,240 - BWI76_RS19180 0.85 +0.2
3,993,296 - BWI76_RS19180 0.89 +1.3
3,993,597 + BWI76_RS19185 0.14 -1.4
3,993,731 - BWI76_RS19185 0.27 +1.0
3,993,743 + BWI76_RS19185 0.28 -0.6
3,993,890 + BWI76_RS19185 0.43 -0.8
3,993,915 - BWI76_RS19185 0.45 +0.6
3,994,031 + BWI76_RS19185 0.56 -0.4
3,994,236 + BWI76_RS19185 0.76 -0.3
3,994,294 + BWI76_RS19185 0.82 +0.8
3,994,373 + BWI76_RS19185 0.89 -1.8
3,994,438 + +0.3

Or see this region's nucleotide sequence