Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16555

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16535 and BWI76_RS16540 are separated by 197 nucleotidesBWI76_RS16540 and BWI76_RS16545 are separated by 4 nucleotidesBWI76_RS16545 and BWI76_RS16550 are separated by 4 nucleotidesBWI76_RS16550 and BWI76_RS16555 are separated by 442 nucleotidesBWI76_RS16555 and BWI76_RS16560 are separated by 90 nucleotidesBWI76_RS16560 and BWI76_RS16565 are separated by 32 nucleotides BWI76_RS16535: BWI76_RS16535 - MATE family efflux transporter, at 3,428,107 to 3,429,480 _RS16535 BWI76_RS16540: BWI76_RS16540 - tRNA-Val, at 3,429,678 to 3,429,754 _RS16540 BWI76_RS16545: BWI76_RS16545 - tRNA-Val, at 3,429,759 to 3,429,835 _RS16545 BWI76_RS16550: BWI76_RS16550 - tRNA-Val, at 3,429,840 to 3,429,916 _RS16550 BWI76_RS16555: BWI76_RS16555 - hypothetical protein, at 3,430,359 to 3,430,544 _RS16555 BWI76_RS16560: BWI76_RS16560 - YciE/YciF family protein, at 3,430,635 to 3,431,132 _RS16560 BWI76_RS16565: BWI76_RS16565 - YciE/YciF family protein, at 3,431,165 to 3,431,671 _RS16565 Position (kb) 3430 3431Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3429.364 kb on + strandat 3429.446 kb on - strandat 3429.857 kb on + strand, within BWI76_RS16550at 3429.857 kb on + strand, within BWI76_RS16550at 3429.864 kb on + strand, within BWI76_RS16550at 3429.874 kb on + strand, within BWI76_RS16550at 3429.878 kb on + strand, within BWI76_RS16550at 3429.909 kb on + strandat 3430.218 kb on - strandat 3430.272 kb on - strandat 3430.336 kb on + strandat 3430.336 kb on + strandat 3430.368 kb on + strandat 3430.424 kb on + strand, within BWI76_RS16555at 3430.424 kb on + strand, within BWI76_RS16555at 3430.424 kb on + strand, within BWI76_RS16555at 3430.433 kb on - strand, within BWI76_RS16555at 3430.434 kb on + strand, within BWI76_RS16555at 3430.434 kb on + strand, within BWI76_RS16555at 3430.434 kb on + strand, within BWI76_RS16555at 3430.434 kb on + strandat 3430.439 kb on + strand, within BWI76_RS16555at 3430.439 kb on + strand, within BWI76_RS16555at 3430.444 kb on + strand, within BWI76_RS16555at 3430.444 kb on + strand, within BWI76_RS16555at 3430.592 kb on + strandat 3430.623 kb on + strandat 3430.727 kb on + strand, within BWI76_RS16560at 3430.735 kb on - strand, within BWI76_RS16560at 3430.735 kb on - strand, within BWI76_RS16560at 3430.735 kb on - strand, within BWI76_RS16560at 3430.749 kb on + strand, within BWI76_RS16560at 3430.751 kb on + strand, within BWI76_RS16560at 3430.751 kb on + strand, within BWI76_RS16560at 3430.751 kb on + strand, within BWI76_RS16560at 3430.751 kb on + strand, within BWI76_RS16560at 3430.751 kb on + strand, within BWI76_RS16560at 3430.751 kb on + strand, within BWI76_RS16560at 3430.751 kb on + strand, within BWI76_RS16560at 3430.751 kb on + strand, within BWI76_RS16560at 3430.751 kb on + strandat 3430.759 kb on - strand, within BWI76_RS16560at 3430.759 kb on - strand, within BWI76_RS16560at 3430.759 kb on - strand, within BWI76_RS16560at 3430.759 kb on - strand, within BWI76_RS16560at 3430.759 kb on - strand, within BWI76_RS16560at 3430.759 kb on - strand, within BWI76_RS16560at 3430.759 kb on - strand, within BWI76_RS16560at 3430.759 kb on - strand, within BWI76_RS16560at 3430.759 kb on - strand, within BWI76_RS16560at 3430.771 kb on - strand, within BWI76_RS16560at 3430.771 kb on - strand, within BWI76_RS16560at 3430.771 kb on - strand, within BWI76_RS16560at 3430.774 kb on + strand, within BWI76_RS16560at 3430.774 kb on + strand, within BWI76_RS16560at 3430.774 kb on + strand, within BWI76_RS16560at 3430.774 kb on + strand, within BWI76_RS16560at 3430.774 kb on + strand, within BWI76_RS16560at 3430.782 kb on - strand, within BWI76_RS16560at 3430.782 kb on - strandat 3430.782 kb on - strand, within BWI76_RS16560at 3430.782 kb on - strandat 3430.783 kb on - strand, within BWI76_RS16560at 3430.783 kb on - strand, within BWI76_RS16560at 3430.792 kb on - strand, within BWI76_RS16560at 3430.792 kb on - strand, within BWI76_RS16560at 3430.794 kb on - strand, within BWI76_RS16560at 3430.794 kb on - strand, within BWI76_RS16560at 3430.794 kb on - strand, within BWI76_RS16560at 3430.804 kb on - strand, within BWI76_RS16560at 3430.804 kb on - strand, within BWI76_RS16560at 3430.863 kb on + strand, within BWI76_RS16560at 3430.904 kb on - strand, within BWI76_RS16560at 3430.992 kb on + strand, within BWI76_RS16560at 3430.997 kb on - strand, within BWI76_RS16560at 3431.007 kb on - strand, within BWI76_RS16560at 3431.018 kb on - strand, within BWI76_RS16560at 3431.180 kb on - strandat 3431.262 kb on + strand, within BWI76_RS16565at 3431.268 kb on + strand, within BWI76_RS16565at 3431.268 kb on + strand, within BWI76_RS16565at 3431.268 kb on + strand, within BWI76_RS16565at 3431.268 kb on + strand, within BWI76_RS16565at 3431.268 kb on + strand, within BWI76_RS16565at 3431.268 kb on + strand, within BWI76_RS16565at 3431.268 kb on + strand, within BWI76_RS16565at 3431.268 kb on + strand, within BWI76_RS16565at 3431.268 kb on + strand, within BWI76_RS16565at 3431.268 kb on + strand, within BWI76_RS16565at 3431.273 kb on + strand, within BWI76_RS16565at 3431.276 kb on - strand, within BWI76_RS16565at 3431.276 kb on - strand, within BWI76_RS16565at 3431.276 kb on - strandat 3431.276 kb on - strand, within BWI76_RS16565at 3431.276 kb on - strand, within BWI76_RS16565at 3431.276 kb on - strand, within BWI76_RS16565at 3431.286 kb on + strandat 3431.286 kb on + strand, within BWI76_RS16565at 3431.286 kb on + strand, within BWI76_RS16565at 3431.286 kb on + strand, within BWI76_RS16565at 3431.294 kb on - strand, within BWI76_RS16565at 3431.296 kb on + strand, within BWI76_RS16565at 3431.296 kb on + strand, within BWI76_RS16565at 3431.369 kb on + strand, within BWI76_RS16565at 3431.369 kb on + strand, within BWI76_RS16565at 3431.369 kb on + strand, within BWI76_RS16565at 3431.369 kb on + strand, within BWI76_RS16565at 3431.375 kb on - strand, within BWI76_RS16565at 3431.377 kb on - strand, within BWI76_RS16565at 3431.400 kb on + strand, within BWI76_RS16565at 3431.400 kb on + strand, within BWI76_RS16565at 3431.408 kb on - strand, within BWI76_RS16565at 3431.421 kb on - strand, within BWI76_RS16565at 3431.433 kb on - strand, within BWI76_RS16565at 3431.469 kb on + strand, within BWI76_RS16565at 3431.479 kb on + strand, within BWI76_RS16565at 3431.479 kb on + strand, within BWI76_RS16565at 3431.479 kb on + strand, within BWI76_RS16565at 3431.479 kb on + strand, within BWI76_RS16565at 3431.500 kb on - strand, within BWI76_RS16565at 3431.500 kb on - strand, within BWI76_RS16565at 3431.518 kb on + strand, within BWI76_RS16565at 3431.526 kb on - strand, within BWI76_RS16565at 3431.526 kb on - strand, within BWI76_RS16565at 3431.526 kb on - strand, within BWI76_RS16565

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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3,429,364 + -0.0
3,429,446 - +1.0
3,429,857 + BWI76_RS16550 0.22 -2.2
3,429,857 + BWI76_RS16550 0.22 -0.3
3,429,864 + BWI76_RS16550 0.31 -0.2
3,429,874 + BWI76_RS16550 0.44 -1.6
3,429,878 + BWI76_RS16550 0.49 -1.6
3,429,909 + -1.4
3,430,218 - -1.4
3,430,272 - +2.0
3,430,336 + -0.6
3,430,336 + +0.8
3,430,368 + +1.7
3,430,424 + BWI76_RS16555 0.35 -2.8
3,430,424 + BWI76_RS16555 0.35 -0.8
3,430,424 + BWI76_RS16555 0.35 -1.0
3,430,433 - BWI76_RS16555 0.40 +2.2
3,430,434 + BWI76_RS16555 0.40 -1.0
3,430,434 + BWI76_RS16555 0.40 -2.8
3,430,434 + BWI76_RS16555 0.40 -2.4
3,430,434 + +1.0
3,430,439 + BWI76_RS16555 0.43 -2.4
3,430,439 + BWI76_RS16555 0.43 -2.7
3,430,444 + BWI76_RS16555 0.46 -0.0
3,430,444 + BWI76_RS16555 0.46 -1.0
3,430,592 + -2.2
3,430,623 + -1.4
3,430,727 + BWI76_RS16560 0.18 -1.5
3,430,735 - BWI76_RS16560 0.20 -1.0
3,430,735 - BWI76_RS16560 0.20 -0.5
3,430,735 - BWI76_RS16560 0.20 +2.0
3,430,749 + BWI76_RS16560 0.23 -1.0
3,430,751 + BWI76_RS16560 0.23 -1.4
3,430,751 + BWI76_RS16560 0.23 -1.4
3,430,751 + BWI76_RS16560 0.23 -2.7
3,430,751 + BWI76_RS16560 0.23 -0.0
3,430,751 + BWI76_RS16560 0.23 -0.6
3,430,751 + BWI76_RS16560 0.23 +0.5
3,430,751 + BWI76_RS16560 0.23 -1.1
3,430,751 + BWI76_RS16560 0.23 -1.6
3,430,751 + -0.6
3,430,759 - BWI76_RS16560 0.25 -1.1
3,430,759 - BWI76_RS16560 0.25 -0.7
3,430,759 - BWI76_RS16560 0.25 +3.3
3,430,759 - BWI76_RS16560 0.25 -1.6
3,430,759 - BWI76_RS16560 0.25 +2.0
3,430,759 - BWI76_RS16560 0.25 +1.3
3,430,759 - BWI76_RS16560 0.25 -1.8
3,430,759 - BWI76_RS16560 0.25 -0.6
3,430,759 - BWI76_RS16560 0.25 +0.5
3,430,771 - BWI76_RS16560 0.27 -0.2
3,430,771 - BWI76_RS16560 0.27 +0.2
3,430,771 - BWI76_RS16560 0.27 -0.6
3,430,774 + BWI76_RS16560 0.28 -0.6
3,430,774 + BWI76_RS16560 0.28 -0.0
3,430,774 + BWI76_RS16560 0.28 -1.6
3,430,774 + BWI76_RS16560 0.28 -1.3
3,430,774 + BWI76_RS16560 0.28 -0.6
3,430,782 - BWI76_RS16560 0.30 +0.2
3,430,782 - +1.0
3,430,782 - BWI76_RS16560 0.30 -2.5
3,430,782 - -1.0
3,430,783 - BWI76_RS16560 0.30 +3.0
3,430,783 - BWI76_RS16560 0.30 -2.6
3,430,792 - BWI76_RS16560 0.32 -0.0
3,430,792 - BWI76_RS16560 0.32 -1.9
3,430,794 - BWI76_RS16560 0.32 +1.0
3,430,794 - BWI76_RS16560 0.32 -2.2
3,430,794 - BWI76_RS16560 0.32 +0.3
3,430,804 - BWI76_RS16560 0.34 -2.2
3,430,804 - BWI76_RS16560 0.34 -0.3
3,430,863 + BWI76_RS16560 0.46 -3.6
3,430,904 - BWI76_RS16560 0.54 -0.6
3,430,992 + BWI76_RS16560 0.72 -1.0
3,430,997 - BWI76_RS16560 0.73 -0.8
3,431,007 - BWI76_RS16560 0.75 -0.4
3,431,018 - BWI76_RS16560 0.77 -0.2
3,431,180 - -0.5
3,431,262 + BWI76_RS16565 0.19 -3.6
3,431,268 + BWI76_RS16565 0.20 -0.6
3,431,268 + BWI76_RS16565 0.20 +0.4
3,431,268 + BWI76_RS16565 0.20 +1.5
3,431,268 + BWI76_RS16565 0.20 -1.2
3,431,268 + BWI76_RS16565 0.20 +0.4
3,431,268 + BWI76_RS16565 0.20 -2.8
3,431,268 + BWI76_RS16565 0.20 +0.3
3,431,268 + BWI76_RS16565 0.20 +0.1
3,431,268 + BWI76_RS16565 0.20 -1.4
3,431,268 + BWI76_RS16565 0.20 +0.2
3,431,273 + BWI76_RS16565 0.21 +0.9
3,431,276 - BWI76_RS16565 0.22 -2.5
3,431,276 - BWI76_RS16565 0.22 +1.5
3,431,276 - -0.6
3,431,276 - BWI76_RS16565 0.22 +1.4
3,431,276 - BWI76_RS16565 0.22 -2.7
3,431,276 - BWI76_RS16565 0.22 -0.6
3,431,286 + +1.0
3,431,286 + BWI76_RS16565 0.24 +0.6
3,431,286 + BWI76_RS16565 0.24 -2.0
3,431,286 + BWI76_RS16565 0.24 -0.6
3,431,294 - BWI76_RS16565 0.25 +1.2
3,431,296 + BWI76_RS16565 0.26 -1.4
3,431,296 + BWI76_RS16565 0.26 -0.0
3,431,369 + BWI76_RS16565 0.40 -0.6
3,431,369 + BWI76_RS16565 0.40 -0.7
3,431,369 + BWI76_RS16565 0.40 -1.6
3,431,369 + BWI76_RS16565 0.40 +1.0
3,431,375 - BWI76_RS16565 0.41 -0.6
3,431,377 - BWI76_RS16565 0.42 -2.4
3,431,400 + BWI76_RS16565 0.46 -3.6
3,431,400 + BWI76_RS16565 0.46 -2.4
3,431,408 - BWI76_RS16565 0.48 -0.8
3,431,421 - BWI76_RS16565 0.50 +0.6
3,431,433 - BWI76_RS16565 0.53 -1.0
3,431,469 + BWI76_RS16565 0.60 +0.1
3,431,479 + BWI76_RS16565 0.62 +1.0
3,431,479 + BWI76_RS16565 0.62 -0.8
3,431,479 + BWI76_RS16565 0.62 +0.1
3,431,479 + BWI76_RS16565 0.62 +0.3
3,431,500 - BWI76_RS16565 0.66 +0.4
3,431,500 - BWI76_RS16565 0.66 +0.3
3,431,518 + BWI76_RS16565 0.70 -0.6
3,431,526 - BWI76_RS16565 0.71 +1.0
3,431,526 - BWI76_RS16565 0.71 -0.3
3,431,526 - BWI76_RS16565 0.71 -0.3

Or see this region's nucleotide sequence