Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11915 and BWI76_RS11920 are separated by 56 nucleotides BWI76_RS11920 and BWI76_RS11925 are separated by 410 nucleotides BWI76_RS11925 and BWI76_RS11930 are separated by 329 nucleotides
BWI76_RS11915: BWI76_RS11915 - protease SohB, at 2,506,820 to 2,507,911
_RS11915
BWI76_RS11920: BWI76_RS11920 - hypothetical protein, at 2,507,968 to 2,508,219
_RS11920
BWI76_RS11925: BWI76_RS11925 - DNA topoisomerase I, at 2,508,630 to 2,511,227
_RS11925
BWI76_RS11930: BWI76_RS11930 - LysR family transcriptional regulator CysB, at 2,511,557 to 2,512,531
_RS11930
Position (kb)
2508
2509
2510
2511
2512 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2507.666 kb on - strand, within BWI76_RS11915 at 2507.885 kb on - strand at 2507.946 kb on - strand at 2508.058 kb on - strand, within BWI76_RS11920 at 2508.082 kb on + strand, within BWI76_RS11920 at 2508.106 kb on - strand, within BWI76_RS11920 at 2508.106 kb on - strand, within BWI76_RS11920 at 2508.255 kb on - strand at 2508.383 kb on + strand at 2508.521 kb on + strand at 2511.326 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,507,666 - BWI76_RS11915 0.77 +0.6 2,507,885 - +0.9 2,507,946 - -0.2 2,508,058 - BWI76_RS11920 0.36 +3.1 2,508,082 + BWI76_RS11920 0.45 +1.0 2,508,106 - BWI76_RS11920 0.55 +1.2 2,508,106 - BWI76_RS11920 0.55 +0.6 2,508,255 - +1.2 2,508,383 + -3.3 2,508,521 + -2.9 2,511,326 - +2.5
Or see this region's nucleotide sequence