Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11240 and BWI76_RS11245 overlap by 4 nucleotides BWI76_RS11245 and BWI76_RS11250 overlap by 4 nucleotides BWI76_RS11250 and BWI76_RS11255 overlap by 17 nucleotides
BWI76_RS11240: BWI76_RS11240 - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 2,371,207 to 2,372,253
_RS11240
BWI76_RS11245: BWI76_RS11245 - spermidine/putrescine ABC transporter permease PotC, at 2,372,250 to 2,373,035
_RS11245
BWI76_RS11250: BWI76_RS11250 - spermidine/putrescine ABC transporter permease PotB, at 2,373,032 to 2,373,889
_RS11250
BWI76_RS11255: BWI76_RS11255 - putrescine/spermidine ABC transporter ATP-binding protein, at 2,373,873 to 2,375,009
_RS11255
Position (kb)
2373
2374 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2372.461 kb on - strand, within BWI76_RS11245 at 2372.599 kb on - strand, within BWI76_RS11245 at 2372.922 kb on + strand, within BWI76_RS11245 at 2373.411 kb on + strand, within BWI76_RS11250 at 2373.732 kb on - strand, within BWI76_RS11250 at 2373.837 kb on - strand at 2374.067 kb on - strand, within BWI76_RS11255 at 2374.067 kb on - strand, within BWI76_RS11255 at 2374.067 kb on - strand, within BWI76_RS11255
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,372,461 - BWI76_RS11245 0.27 +0.1 2,372,599 - BWI76_RS11245 0.44 +0.5 2,372,922 + BWI76_RS11245 0.85 -0.8 2,373,411 + BWI76_RS11250 0.44 -1.6 2,373,732 - BWI76_RS11250 0.82 -1.5 2,373,837 - -1.0 2,374,067 - BWI76_RS11255 0.17 -3.5 2,374,067 - BWI76_RS11255 0.17 -2.6 2,374,067 - BWI76_RS11255 0.17 -0.6
Or see this region's nucleotide sequence