Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08825

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08815 and BWI76_RS08820 are separated by 11 nucleotidesBWI76_RS08820 and BWI76_RS08825 are separated by 3 nucleotidesBWI76_RS08825 and BWI76_RS08830 overlap by 8 nucleotidesBWI76_RS08830 and BWI76_RS08835 are separated by 1 nucleotidesBWI76_RS08835 and BWI76_RS08840 are separated by 44 nucleotides BWI76_RS08815: BWI76_RS08815 - cyclic pyranopterin phosphate synthase, at 1,858,509 to 1,859,508 _RS08815 BWI76_RS08820: BWI76_RS08820 - molybdenum cofactor biosynthesis protein, at 1,859,520 to 1,860,032 _RS08820 BWI76_RS08825: BWI76_RS08825 - cyclic pyranopterin monophosphate synthase MoaC, at 1,860,036 to 1,860,524 _RS08825 BWI76_RS08830: BWI76_RS08830 - molybdopterin synthase sulfur carrier subunit, at 1,860,517 to 1,860,765 _RS08830 BWI76_RS08835: BWI76_RS08835 - molybdopterin guanine dinucleotide biosynthesis protein MoaE, at 1,860,767 to 1,861,219 _RS08835 BWI76_RS08840: BWI76_RS08840 - hypothetical protein, at 1,861,264 to 1,861,968 _RS08840 Position (kb) 1860 1861Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1859.062 kb on + strand, within BWI76_RS08815at 1859.252 kb on + strand, within BWI76_RS08815at 1859.327 kb on + strand, within BWI76_RS08815at 1859.536 kb on - strandat 1859.592 kb on + strand, within BWI76_RS08820at 1859.821 kb on - strand, within BWI76_RS08820at 1859.866 kb on - strand, within BWI76_RS08820at 1859.965 kb on + strand, within BWI76_RS08820at 1860.154 kb on - strand, within BWI76_RS08825at 1860.268 kb on - strand, within BWI76_RS08825at 1860.425 kb on - strand, within BWI76_RS08825at 1860.563 kb on - strand, within BWI76_RS08830at 1860.768 kb on - strandat 1860.825 kb on - strand, within BWI76_RS08835at 1860.886 kb on + strand, within BWI76_RS08835at 1860.926 kb on - strand, within BWI76_RS08835at 1860.928 kb on - strand, within BWI76_RS08835at 1861.135 kb on + strand, within BWI76_RS08835at 1861.327 kb on + strandat 1861.355 kb on + strand, within BWI76_RS08840

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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1,859,062 + BWI76_RS08815 0.55 +0.8
1,859,252 + BWI76_RS08815 0.74 -0.3
1,859,327 + BWI76_RS08815 0.82 +0.8
1,859,536 - -1.9
1,859,592 + BWI76_RS08820 0.14 -3.5
1,859,821 - BWI76_RS08820 0.59 -0.8
1,859,866 - BWI76_RS08820 0.67 +1.4
1,859,965 + BWI76_RS08820 0.87 +0.2
1,860,154 - BWI76_RS08825 0.24 +0.8
1,860,268 - BWI76_RS08825 0.47 +0.2
1,860,425 - BWI76_RS08825 0.80 +1.8
1,860,563 - BWI76_RS08830 0.18 -1.9
1,860,768 - +0.1
1,860,825 - BWI76_RS08835 0.13 +0.9
1,860,886 + BWI76_RS08835 0.26 +0.9
1,860,926 - BWI76_RS08835 0.35 -1.0
1,860,928 - BWI76_RS08835 0.36 +0.6
1,861,135 + BWI76_RS08835 0.81 +0.4
1,861,327 + +0.3
1,861,355 + BWI76_RS08840 0.13 +1.2

Or see this region's nucleotide sequence