Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04740

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04735 and BWI76_RS04740 overlap by 4 nucleotidesBWI76_RS04740 and BWI76_RS04745 overlap by 7 nucleotides BWI76_RS04735: BWI76_RS04735 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase, at 987,457 to 988,944 _RS04735 BWI76_RS04740: BWI76_RS04740 - UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase, at 988,941 to 990,299 _RS04740 BWI76_RS04745: BWI76_RS04745 - phospho-N-acetylmuramoyl-pentapeptide- transferase, at 990,293 to 991,375 _RS04745 Position (kb) 988 989 990 991Strain fitness (log2 ratio) -1 0 1at 990.274 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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990,274 + +0.0

Or see this region's nucleotide sequence