Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04680 and BWI76_RS04685 are separated by 95 nucleotides BWI76_RS04685 and BWI76_RS04690 are separated by 621 nucleotides
BWI76_RS04680: BWI76_RS04680 - 2-isopropylmalate synthase, at 976,179 to 977,750
_RS04680
BWI76_RS04685: BWI76_RS04685 - putative leucine operon leader peptide, at 977,846 to 977,932
_RS04685
BWI76_RS04690: BWI76_RS04690 - transcriptional regulator LeuO, at 978,554 to 979,504
_RS04690
Position (kb) 977
978 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 976.858 kb on + strand, within BWI76_RS04680 at 976.895 kb on - strand, within BWI76_RS04680 at 976.918 kb on + strand, within BWI76_RS04680 at 977.079 kb on - strand, within BWI76_RS04680 at 977.084 kb on - strand, within BWI76_RS04680 at 977.091 kb on + strand, within BWI76_RS04680 at 977.104 kb on - strand, within BWI76_RS04680 at 977.135 kb on - strand, within BWI76_RS04680 at 977.172 kb on + strand, within BWI76_RS04680 at 977.222 kb on + strand, within BWI76_RS04680 at 977.222 kb on + strand, within BWI76_RS04680 at 977.234 kb on - strand, within BWI76_RS04680 at 977.361 kb on - strand, within BWI76_RS04680 at 977.361 kb on - strand, within BWI76_RS04680 at 977.450 kb on + strand, within BWI76_RS04680 at 977.550 kb on + strand, within BWI76_RS04680 at 977.550 kb on + strand, within BWI76_RS04680 at 977.571 kb on + strand, within BWI76_RS04680 at 977.572 kb on - strand, within BWI76_RS04680 at 977.579 kb on - strand, within BWI76_RS04680 at 977.627 kb on + strand at 977.627 kb on + strand at 977.632 kb on + strand at 977.686 kb on - strand at 977.688 kb on + strand at 977.688 kb on + strand at 977.702 kb on + strand at 977.707 kb on - strand at 977.707 kb on - strand at 977.710 kb on - strand at 977.712 kb on + strand at 977.712 kb on + strand at 977.720 kb on - strand at 977.720 kb on - strand at 977.760 kb on - strand at 977.760 kb on - strand at 977.769 kb on + strand at 977.771 kb on + strand at 977.777 kb on - strand at 977.781 kb on - strand at 977.820 kb on + strand at 977.844 kb on + strand at 977.859 kb on + strand, within BWI76_RS04685 at 977.866 kb on + strand, within BWI76_RS04685 at 977.866 kb on + strand, within BWI76_RS04685 at 977.874 kb on - strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on - strand, within BWI76_RS04685 at 977.899 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.950 kb on - strand at 977.950 kb on - strand at 977.970 kb on - strand at 977.970 kb on - strand at 977.970 kb on - strand at 977.970 kb on - strand at 977.977 kb on + strand at 977.977 kb on + strand at 977.977 kb on + strand at 977.985 kb on - strand at 977.990 kb on + strand at 977.991 kb on + strand at 977.997 kb on + strand at 978.004 kb on - strand at 978.005 kb on - strand at 978.047 kb on + strand at 978.055 kb on - strand at 978.057 kb on - strand at 978.155 kb on - strand at 978.167 kb on - strand at 978.169 kb on + strand at 978.169 kb on + strand at 978.177 kb on - strand at 978.177 kb on - strand at 978.178 kb on - strand at 978.221 kb on - strand at 978.271 kb on + strand at 978.271 kb on + strand at 978.271 kb on + strand at 978.271 kb on + strand at 978.279 kb on - strand at 978.377 kb on + strand at 978.377 kb on + strand at 978.377 kb on + strand at 978.431 kb on + strand at 978.431 kb on - strand at 978.548 kb on - strand at 978.550 kb on - strand at 978.585 kb on + strand at 978.596 kb on + strand at 978.600 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.606 kb on + strand at 978.606 kb on + strand at 978.608 kb on + strand at 978.608 kb on + strand at 978.608 kb on + strand at 978.608 kb on + strand at 978.608 kb on - strand at 978.608 kb on - strand at 978.609 kb on - strand at 978.609 kb on - strand at 978.616 kb on - strand at 978.616 kb on - strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.635 kb on - strand at 978.693 kb on + strand, within BWI76_RS04690 at 978.700 kb on - strand, within BWI76_RS04690 at 978.700 kb on - strand, within BWI76_RS04690 at 978.701 kb on - strand, within BWI76_RS04690 at 978.703 kb on + strand, within BWI76_RS04690 at 978.713 kb on + strand at 978.713 kb on + strand, within BWI76_RS04690 at 978.713 kb on + strand, within BWI76_RS04690 at 978.713 kb on + strand, within BWI76_RS04690 at 978.721 kb on - strand, within BWI76_RS04690 at 978.728 kb on - strand, within BWI76_RS04690 at 978.728 kb on - strand, within BWI76_RS04690 at 978.756 kb on - strand, within BWI76_RS04690 at 978.783 kb on + strand, within BWI76_RS04690 at 978.789 kb on + strand, within BWI76_RS04690 at 978.789 kb on + strand, within BWI76_RS04690 at 978.791 kb on - strand, within BWI76_RS04690 at 978.814 kb on + strand, within BWI76_RS04690 at 978.819 kb on + strand, within BWI76_RS04690 at 978.822 kb on - strand, within BWI76_RS04690 at 978.827 kb on - strand, within BWI76_RS04690 at 978.840 kb on + strand, within BWI76_RS04690 at 978.845 kb on + strand, within BWI76_RS04690 at 978.863 kb on + strand, within BWI76_RS04690 at 978.870 kb on + strand, within BWI76_RS04690 at 978.870 kb on + strand, within BWI76_RS04690 at 978.878 kb on - strand, within BWI76_RS04690 at 978.878 kb on - strand, within BWI76_RS04690 at 978.880 kb on + strand, within BWI76_RS04690 at 978.880 kb on + strand, within BWI76_RS04690 at 978.880 kb on + strand, within BWI76_RS04690 at 978.880 kb on + strand, within BWI76_RS04690 at 978.888 kb on - strand, within BWI76_RS04690 at 978.888 kb on - strand, within BWI76_RS04690 at 978.888 kb on - strand, within BWI76_RS04690 at 978.888 kb on - strand, within BWI76_RS04690 at 978.888 kb on - strand, within BWI76_RS04690 at 978.888 kb on - strand, within BWI76_RS04690 at 978.918 kb on - strand, within BWI76_RS04690 at 978.920 kb on + strand, within BWI76_RS04690 at 978.925 kb on + strand, within BWI76_RS04690
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 976,858 + BWI76_RS04680 0.43 -6.5 976,895 - BWI76_RS04680 0.46 -1.8 976,918 + BWI76_RS04680 0.47 +1.1 977,079 - BWI76_RS04680 0.57 -0.4 977,084 - BWI76_RS04680 0.58 -4.0 977,091 + BWI76_RS04680 0.58 -2.7 977,104 - BWI76_RS04680 0.59 -5.4 977,135 - BWI76_RS04680 0.61 +0.1 977,172 + BWI76_RS04680 0.63 -2.4 977,222 + BWI76_RS04680 0.66 -3.2 977,222 + BWI76_RS04680 0.66 -3.9 977,234 - BWI76_RS04680 0.67 -1.6 977,361 - BWI76_RS04680 0.75 -2.9 977,361 - BWI76_RS04680 0.75 +0.1 977,450 + BWI76_RS04680 0.81 -1.2 977,550 + BWI76_RS04680 0.87 -5.6 977,550 + BWI76_RS04680 0.87 +1.1 977,571 + BWI76_RS04680 0.89 -4.0 977,572 - BWI76_RS04680 0.89 -5.4 977,579 - BWI76_RS04680 0.89 -3.3 977,627 + +0.7 977,627 + -4.0 977,632 + -4.1 977,686 - -2.5 977,688 + -5.2 977,688 + -1.7 977,702 + -5.6 977,707 - -4.4 977,707 - +0.1 977,710 - -4.0 977,712 + -0.4 977,712 + -4.2 977,720 - -0.9 977,720 - -3.4 977,760 - -2.9 977,760 - -3.0 977,769 + -3.9 977,771 + -4.9 977,777 - -0.6 977,781 - -4.4 977,820 + -1.4 977,844 + -3.8 977,859 + BWI76_RS04685 0.15 -5.5 977,866 + BWI76_RS04685 0.23 -1.4 977,866 + BWI76_RS04685 0.23 +1.1 977,874 - BWI76_RS04685 0.32 -4.9 977,899 + BWI76_RS04685 0.61 -2.2 977,899 + BWI76_RS04685 0.61 -2.4 977,899 + BWI76_RS04685 0.61 -3.9 977,899 + BWI76_RS04685 0.61 -5.5 977,899 - BWI76_RS04685 0.61 -4.7 977,899 - BWI76_RS04685 0.61 +1.1 977,907 - BWI76_RS04685 0.70 +0.1 977,907 - BWI76_RS04685 0.70 -3.2 977,907 - BWI76_RS04685 0.70 -2.8 977,907 - BWI76_RS04685 0.70 -3.7 977,907 - BWI76_RS04685 0.70 -3.3 977,950 - -0.4 977,950 - -2.9 977,970 - -0.8 977,970 - -1.1 977,970 - +0.6 977,970 - -3.8 977,977 + +1.1 977,977 + -3.7 977,977 + -1.7 977,985 - +0.6 977,990 + -1.2 977,991 + +1.4 977,997 + +0.0 978,004 - +0.3 978,005 - -1.7 978,047 + +0.5 978,055 - -1.2 978,057 - -2.2 978,155 - -0.4 978,167 - -1.4 978,169 + -2.7 978,169 + -0.4 978,177 - +0.1 978,177 - -2.4 978,178 - -3.0 978,221 - -0.4 978,271 + +0.6 978,271 + -1.9 978,271 + +1.1 978,271 + -0.4 978,279 - -0.4 978,377 + -0.2 978,377 + -3.2 978,377 + -2.0 978,431 + +0.1 978,431 - -2.8 978,548 - -1.4 978,550 - -0.4 978,585 + -0.9 978,596 + +1.1 978,600 + +1.8 978,601 + -1.7 978,601 + +1.1 978,601 + +0.2 978,601 + +0.0 978,601 + -1.9 978,601 + -1.4 978,606 + -2.0 978,606 + +0.6 978,608 + -0.9 978,608 + -0.7 978,608 + -0.7 978,608 + +1.7 978,608 - -2.2 978,608 - -3.7 978,609 - -1.9 978,609 - +1.1 978,616 - -1.9 978,616 - -0.9 978,627 + +0.1 978,627 + +1.1 978,627 + -0.9 978,627 + -1.2 978,627 + -1.2 978,635 - +1.1 978,693 + BWI76_RS04690 0.15 -0.8 978,700 - BWI76_RS04690 0.15 -1.3 978,700 - BWI76_RS04690 0.15 +0.3 978,701 - BWI76_RS04690 0.15 -1.6 978,703 + BWI76_RS04690 0.16 +0.9 978,713 + +1.1 978,713 + BWI76_RS04690 0.17 -0.9 978,713 + BWI76_RS04690 0.17 -0.4 978,713 + BWI76_RS04690 0.17 +1.1 978,721 - BWI76_RS04690 0.18 -0.4 978,728 - BWI76_RS04690 0.18 +1.0 978,728 - BWI76_RS04690 0.18 +0.3 978,756 - BWI76_RS04690 0.21 +0.2 978,783 + BWI76_RS04690 0.24 -2.6 978,789 + BWI76_RS04690 0.25 -2.0 978,789 + BWI76_RS04690 0.25 +1.9 978,791 - BWI76_RS04690 0.25 -2.3 978,814 + BWI76_RS04690 0.27 +0.9 978,819 + BWI76_RS04690 0.28 -2.8 978,822 - BWI76_RS04690 0.28 +0.1 978,827 - BWI76_RS04690 0.29 +0.3 978,840 + BWI76_RS04690 0.30 -0.9 978,845 + BWI76_RS04690 0.31 -2.6 978,863 + BWI76_RS04690 0.32 +2.1 978,870 + BWI76_RS04690 0.33 -1.4 978,870 + BWI76_RS04690 0.33 +0.1 978,878 - BWI76_RS04690 0.34 +1.1 978,878 - BWI76_RS04690 0.34 +0.1 978,880 + BWI76_RS04690 0.34 -0.1 978,880 + BWI76_RS04690 0.34 +0.1 978,880 + BWI76_RS04690 0.34 -1.9 978,880 + BWI76_RS04690 0.34 +1.1 978,888 - BWI76_RS04690 0.35 -1.4 978,888 - BWI76_RS04690 0.35 -4.0 978,888 - BWI76_RS04690 0.35 -0.4 978,888 - BWI76_RS04690 0.35 -1.9 978,888 - BWI76_RS04690 0.35 -2.3 978,888 - BWI76_RS04690 0.35 +1.1 978,918 - BWI76_RS04690 0.38 +0.1 978,920 + BWI76_RS04690 0.38 -0.5 978,925 + BWI76_RS04690 0.39 +0.1
Or see this region's nucleotide sequence