Experiment: MoLS4 with Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yscR and DVUA0123 are separated by 30 nucleotides DVUA0123 and DVUA0124 are separated by 15 nucleotides DVUA0124 and slt are separated by 10 nucleotides slt and DVUA0126 are separated by 373 nucleotides DVUA0126 and DORF2161 are separated by 59 nucleotides
DVUA0122: yscR - type III secretion system protein, YscR family (TIGR), at 161,611 to 162,261
yscR
DVUA0123: DVUA0123 - anti-anti-sigma factor (TIGR), at 162,292 to 162,627
DVUA0123
DVUA0124: DVUA0124 - sigma factor serine-protein kinase (TIGR), at 162,643 to 163,059
DVUA0124
DVUA0125: slt - transglycosylase, SLT family (TIGR), at 163,070 to 164,422
slt
DVUA0126: DVUA0126 - conserved hypothetical protein (TIGR), at 164,796 to 165,263
DVUA0126
DORF2161: DORF2161 - SEED: FIG00606767: hypothetical protein, at 165,323 to 167,803
DORF2161
Position (kb) 163
164
165 Strain fitness (log2 ratio) -2
-1
0
1
2 at 162.093 kb on + strand, within yscR at 162.094 kb on + strand, within yscR at 162.116 kb on + strand, within yscR at 162.174 kb on - strand, within yscR at 162.243 kb on + strand at 162.251 kb on - strand at 162.280 kb on + strand at 162.380 kb on + strand, within DVUA0123 at 162.402 kb on - strand, within DVUA0123 at 162.450 kb on - strand, within DVUA0123 at 162.491 kb on - strand, within DVUA0123 at 162.543 kb on + strand, within DVUA0123 at 163.210 kb on - strand, within slt at 163.216 kb on + strand, within slt at 163.235 kb on - strand, within slt at 163.281 kb on + strand, within slt at 163.384 kb on + strand, within slt at 163.417 kb on + strand, within slt at 163.423 kb on + strand, within slt at 163.436 kb on - strand, within slt at 163.441 kb on - strand, within slt at 163.451 kb on + strand, within slt at 163.536 kb on + strand, within slt at 163.613 kb on + strand, within slt at 163.630 kb on + strand, within slt at 163.635 kb on + strand, within slt at 163.635 kb on + strand, within slt at 163.635 kb on + strand, within slt at 163.635 kb on + strand, within slt at 163.643 kb on - strand, within slt at 163.688 kb on + strand, within slt at 163.733 kb on - strand, within slt at 163.824 kb on + strand, within slt at 163.848 kb on + strand, within slt at 163.958 kb on - strand, within slt at 163.975 kb on - strand, within slt at 164.097 kb on + strand, within slt at 164.180 kb on - strand, within slt at 164.428 kb on + strand at 164.428 kb on + strand at 164.522 kb on + strand at 164.530 kb on - strand at 164.679 kb on - strand at 164.859 kb on + strand, within DVUA0126 at 164.919 kb on + strand, within DVUA0126 at 164.927 kb on - strand, within DVUA0126 at 165.000 kb on + strand, within DVUA0126 at 165.011 kb on + strand, within DVUA0126 at 165.127 kb on + strand, within DVUA0126 at 165.128 kb on + strand, within DVUA0126 at 165.139 kb on + strand, within DVUA0126 at 165.149 kb on - strand, within DVUA0126 at 165.184 kb on - strand, within DVUA0126 at 165.184 kb on - strand, within DVUA0126 at 165.204 kb on - strand, within DVUA0126 at 165.290 kb on + strand at 165.300 kb on - strand at 165.300 kb on - strand at 165.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Thymine (N) remove 162,093 + yscR DVUA0122 0.74 -1.7 162,094 + yscR DVUA0122 0.74 +0.3 162,116 + yscR DVUA0122 0.78 -1.5 162,174 - yscR DVUA0122 0.86 -0.2 162,243 + +0.5 162,251 - -0.1 162,280 + +0.2 162,380 + DVUA0123 0.26 -0.1 162,402 - DVUA0123 0.33 -2.1 162,450 - DVUA0123 0.47 -2.4 162,491 - DVUA0123 0.59 -2.0 162,543 + DVUA0123 0.75 -1.8 163,210 - slt DVUA0125 0.10 -0.8 163,216 + slt DVUA0125 0.11 +0.4 163,235 - slt DVUA0125 0.12 -0.2 163,281 + slt DVUA0125 0.16 +0.4 163,384 + slt DVUA0125 0.23 +0.1 163,417 + slt DVUA0125 0.26 -0.1 163,423 + slt DVUA0125 0.26 +0.0 163,436 - slt DVUA0125 0.27 -1.3 163,441 - slt DVUA0125 0.27 -0.2 163,451 + slt DVUA0125 0.28 -0.1 163,536 + slt DVUA0125 0.34 -1.9 163,613 + slt DVUA0125 0.40 -0.1 163,630 + slt DVUA0125 0.41 +0.4 163,635 + slt DVUA0125 0.42 -0.1 163,635 + slt DVUA0125 0.42 +0.1 163,635 + slt DVUA0125 0.42 -0.9 163,635 + slt DVUA0125 0.42 +2.2 163,643 - slt DVUA0125 0.42 +0.4 163,688 + slt DVUA0125 0.46 +0.5 163,733 - slt DVUA0125 0.49 +0.6 163,824 + slt DVUA0125 0.56 -0.1 163,848 + slt DVUA0125 0.58 +0.5 163,958 - slt DVUA0125 0.66 +0.7 163,975 - slt DVUA0125 0.67 -1.9 164,097 + slt DVUA0125 0.76 +0.4 164,180 - slt DVUA0125 0.82 +0.3 164,428 + -0.1 164,428 + -0.5 164,522 + +0.9 164,530 - -0.3 164,679 - -0.1 164,859 + DVUA0126 0.13 +0.1 164,919 + DVUA0126 0.26 +0.2 164,927 - DVUA0126 0.28 +0.4 165,000 + DVUA0126 0.44 -0.2 165,011 + DVUA0126 0.46 -0.3 165,127 + DVUA0126 0.71 -0.9 165,128 + DVUA0126 0.71 -0.8 165,139 + DVUA0126 0.73 +0.0 165,149 - DVUA0126 0.75 +0.4 165,184 - DVUA0126 0.83 -0.8 165,184 - DVUA0126 0.83 -0.1 165,204 - DVUA0126 0.87 +0.1 165,290 + -0.3 165,300 - +0.0 165,300 - -0.5 165,403 - -1.2
Or see this region's nucleotide sequence