Experiment: MoLS4 with TEMED 20mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DORF123 and DVUA0019 are separated by 578 nucleotides DVUA0019 and DVUA0020 overlap by 1 nucleotides DVUA0020 and DVUA0020.1 are separated by 154 nucleotides
DORF123: DORF123 - SEED: FIG00603348: hypothetical protein, at 21,989 to 22,207
DORF123
DVUA0019: DVUA0019 - type II DNA modification methyltransferase, putative (TIGR), at 22,786 to 24,444
DVUA0019
DVUA0020: DVUA0020 - type II restriction endonuclease, putative (TIGR), at 24,444 to 25,184
DVUA0020
DVUA0020.1: DVUA0020.1 - SEED: FIG00604339: hypothetical protein, at 25,339 to 26,583
DVUA0020.1
Position (kb)
22
23
24
25 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 21.859 kb on + strand at 21.922 kb on - strand at 21.948 kb on + strand at 22.013 kb on - strand, within DORF123 at 22.013 kb on - strand, within DORF123 at 22.024 kb on - strand, within DORF123 at 22.024 kb on - strand, within DORF123 at 22.077 kb on - strand, within DORF123 at 22.077 kb on - strand, within DORF123 at 22.077 kb on - strand, within DORF123 at 22.079 kb on - strand, within DORF123 at 22.084 kb on + strand, within DORF123 at 22.084 kb on + strand, within DORF123 at 22.092 kb on - strand, within DORF123 at 22.101 kb on + strand, within DORF123 at 22.220 kb on - strand at 22.226 kb on + strand at 22.226 kb on - strand at 22.233 kb on - strand at 22.234 kb on - strand at 22.363 kb on + strand at 22.386 kb on - strand at 22.397 kb on + strand at 22.397 kb on - strand at 22.405 kb on - strand at 22.659 kb on - strand at 24.479 kb on + strand at 24.487 kb on - strand at 24.578 kb on - strand, within DVUA0020 at 24.727 kb on - strand, within DVUA0020 at 24.744 kb on - strand, within DVUA0020 at 24.846 kb on + strand, within DVUA0020 at 24.854 kb on - strand, within DVUA0020 at 24.878 kb on - strand, within DVUA0020 at 24.936 kb on + strand, within DVUA0020 at 24.974 kb on + strand, within DVUA0020 at 25.040 kb on + strand, within DVUA0020 at 25.310 kb on + strand at 25.339 kb on + strand at 25.343 kb on - strand at 25.379 kb on - strand at 25.379 kb on - strand at 25.443 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with TEMED 20mM (N) remove 21,859 + -0.3 21,922 - -0.2 21,948 + -0.3 22,013 - DORF123 0.11 +1.5 22,013 - DORF123 0.11 +0.1 22,024 - DORF123 0.16 -0.6 22,024 - DORF123 0.16 -1.3 22,077 - DORF123 0.40 +0.6 22,077 - DORF123 0.40 +0.2 22,077 - DORF123 0.40 -0.3 22,079 - DORF123 0.41 -0.5 22,084 + DORF123 0.43 +0.4 22,084 + DORF123 0.43 -0.2 22,092 - DORF123 0.47 +0.3 22,101 + DORF123 0.51 +0.1 22,220 - +0.4 22,226 + -0.2 22,226 - -0.3 22,233 - -0.5 22,234 - -0.5 22,363 + -1.7 22,386 - -0.4 22,397 + +1.1 22,397 - +0.1 22,405 - +0.5 22,659 - -0.6 24,479 + -0.6 24,487 - -0.3 24,578 - DVUA0020 0.18 -2.7 24,727 - DVUA0020 0.38 +2.7 24,744 - DVUA0020 0.40 -0.2 24,846 + DVUA0020 0.54 -1.9 24,854 - DVUA0020 0.55 +0.1 24,878 - DVUA0020 0.59 -0.1 24,936 + DVUA0020 0.66 +0.4 24,974 + DVUA0020 0.72 -0.2 25,040 + DVUA0020 0.80 -0.7 25,310 + -0.3 25,339 + -0.5 25,343 - -1.4 25,379 - +0.4 25,379 - +0.0 25,443 - -0.7
Or see this region's nucleotide sequence