Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25510 and BWI76_RS25515 are separated by 77 nucleotides BWI76_RS25515 and BWI76_RS25520 overlap by 1 nucleotides BWI76_RS25520 and BWI76_RS25525 are separated by 121 nucleotides BWI76_RS25525 and BWI76_RS25530 are separated by 9 nucleotides
BWI76_RS25510: BWI76_RS25510 - oxidoreductase, at 5,304,013 to 5,304,987
_RS25510
BWI76_RS25515: BWI76_RS25515 - sulfoxide reductase, at 5,305,065 to 5,306,066
_RS25515
BWI76_RS25520: BWI76_RS25520 - sulfoxide reductase heme-binding subunit YedZ, at 5,306,066 to 5,306,665
_RS25520
BWI76_RS25525: BWI76_RS25525 - hypothetical protein, at 5,306,787 to 5,306,987
_RS25525
BWI76_RS25530: BWI76_RS25530 - acetyl-CoA carboxylase, at 5,306,997 to 5,307,086
_RS25530
BWI76_RS25535: BWI76_RS25535 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 5,307,059 to 5,307,532
_RS25535
Position (kb) 5305
5306
5307 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5304.112 kb on + strand, within BWI76_RS25510 at 5304.139 kb on - strand, within BWI76_RS25510 at 5304.248 kb on - strand, within BWI76_RS25510 at 5304.364 kb on - strand, within BWI76_RS25510 at 5304.468 kb on - strand, within BWI76_RS25510 at 5304.756 kb on + strand, within BWI76_RS25510 at 5304.827 kb on - strand, within BWI76_RS25510 at 5304.972 kb on + strand at 5305.021 kb on - strand at 5305.037 kb on - strand at 5305.165 kb on + strand at 5305.165 kb on + strand at 5305.173 kb on - strand, within BWI76_RS25515 at 5305.228 kb on + strand, within BWI76_RS25515 at 5305.264 kb on - strand, within BWI76_RS25515 at 5305.346 kb on + strand, within BWI76_RS25515 at 5305.354 kb on - strand, within BWI76_RS25515 at 5305.354 kb on - strand, within BWI76_RS25515 at 5305.439 kb on + strand, within BWI76_RS25515 at 5305.620 kb on - strand, within BWI76_RS25515 at 5305.716 kb on - strand, within BWI76_RS25515 at 5305.726 kb on - strand at 5305.760 kb on - strand, within BWI76_RS25515 at 5305.837 kb on + strand, within BWI76_RS25515 at 5306.162 kb on - strand, within BWI76_RS25520 at 5306.346 kb on + strand, within BWI76_RS25520 at 5306.346 kb on + strand, within BWI76_RS25520 at 5306.346 kb on + strand, within BWI76_RS25520 at 5306.346 kb on + strand, within BWI76_RS25520 at 5306.346 kb on + strand, within BWI76_RS25520 at 5306.354 kb on - strand, within BWI76_RS25520 at 5306.354 kb on - strand, within BWI76_RS25520 at 5306.354 kb on - strand, within BWI76_RS25520 at 5306.354 kb on - strand, within BWI76_RS25520 at 5306.354 kb on - strand, within BWI76_RS25520 at 5306.386 kb on + strand, within BWI76_RS25520 at 5306.503 kb on + strand, within BWI76_RS25520 at 5306.575 kb on + strand, within BWI76_RS25520
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 5,304,112 + BWI76_RS25510 0.10 +1.4 5,304,139 - BWI76_RS25510 0.13 +0.7 5,304,248 - BWI76_RS25510 0.24 +0.2 5,304,364 - BWI76_RS25510 0.36 +0.4 5,304,468 - BWI76_RS25510 0.47 -0.3 5,304,756 + BWI76_RS25510 0.76 +1.7 5,304,827 - BWI76_RS25510 0.83 +0.7 5,304,972 + -1.3 5,305,021 - +0.2 5,305,037 - +1.3 5,305,165 + -2.8 5,305,165 + -2.4 5,305,173 - BWI76_RS25515 0.11 -0.2 5,305,228 + BWI76_RS25515 0.16 +0.7 5,305,264 - BWI76_RS25515 0.20 -2.9 5,305,346 + BWI76_RS25515 0.28 -1.0 5,305,354 - BWI76_RS25515 0.29 +1.3 5,305,354 - BWI76_RS25515 0.29 -3.5 5,305,439 + BWI76_RS25515 0.37 -2.4 5,305,620 - BWI76_RS25515 0.55 +1.2 5,305,716 - BWI76_RS25515 0.65 +0.6 5,305,726 - +0.7 5,305,760 - BWI76_RS25515 0.69 +0.9 5,305,837 + BWI76_RS25515 0.77 +2.5 5,306,162 - BWI76_RS25520 0.16 +1.3 5,306,346 + BWI76_RS25520 0.47 -1.0 5,306,346 + BWI76_RS25520 0.47 +0.8 5,306,346 + BWI76_RS25520 0.47 -0.3 5,306,346 + BWI76_RS25520 0.47 +0.1 5,306,346 + BWI76_RS25520 0.47 +1.8 5,306,354 - BWI76_RS25520 0.48 +0.1 5,306,354 - BWI76_RS25520 0.48 +1.2 5,306,354 - BWI76_RS25520 0.48 +0.5 5,306,354 - BWI76_RS25520 0.48 -0.4 5,306,354 - BWI76_RS25520 0.48 +0.9 5,306,386 + BWI76_RS25520 0.53 +0.3 5,306,503 + BWI76_RS25520 0.73 -0.8 5,306,575 + BWI76_RS25520 0.85 -1.9
Or see this region's nucleotide sequence