Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24825 and BWI76_RS24830 are separated by 28 nucleotides BWI76_RS24830 and BWI76_RS24835 are separated by 28 nucleotides BWI76_RS24835 and BWI76_RS24840 are separated by 392 nucleotides
BWI76_RS24825: BWI76_RS24825 - 2-hydroxy-3-oxopropionate reductase, at 5,172,634 to 5,173,524
_RS24825
BWI76_RS24830: BWI76_RS24830 - 5-keto-4-deoxy-D-glucarate aldolase, at 5,173,553 to 5,174,323
_RS24830
BWI76_RS24835: BWI76_RS24835 - MFS transporter, at 5,174,352 to 5,175,686
_RS24835
BWI76_RS24840: BWI76_RS24840 - galactarate dehydratase, at 5,176,079 to 5,177,650
_RS24840
Position (kb) 5174
5175
5176 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5173.533 kb on - strand at 5173.636 kb on - strand, within BWI76_RS24830 at 5173.639 kb on + strand, within BWI76_RS24830 at 5173.741 kb on + strand, within BWI76_RS24830 at 5173.777 kb on - strand, within BWI76_RS24830 at 5173.864 kb on - strand, within BWI76_RS24830 at 5173.923 kb on + strand, within BWI76_RS24830 at 5173.923 kb on + strand, within BWI76_RS24830 at 5174.191 kb on - strand, within BWI76_RS24830 at 5174.326 kb on + strand at 5174.334 kb on - strand at 5174.412 kb on + strand at 5174.446 kb on + strand at 5174.487 kb on - strand, within BWI76_RS24835 at 5174.586 kb on + strand, within BWI76_RS24835 at 5174.796 kb on - strand, within BWI76_RS24835 at 5174.814 kb on + strand, within BWI76_RS24835 at 5174.952 kb on - strand, within BWI76_RS24835 at 5175.082 kb on - strand, within BWI76_RS24835 at 5175.103 kb on - strand, within BWI76_RS24835 at 5175.116 kb on + strand, within BWI76_RS24835 at 5175.116 kb on + strand, within BWI76_RS24835 at 5175.208 kb on + strand at 5175.223 kb on + strand, within BWI76_RS24835 at 5175.234 kb on + strand, within BWI76_RS24835 at 5175.330 kb on - strand, within BWI76_RS24835 at 5175.353 kb on + strand, within BWI76_RS24835 at 5175.469 kb on + strand, within BWI76_RS24835 at 5175.506 kb on - strand, within BWI76_RS24835 at 5175.506 kb on - strand, within BWI76_RS24835 at 5175.522 kb on + strand, within BWI76_RS24835 at 5175.530 kb on - strand, within BWI76_RS24835 at 5175.530 kb on - strand, within BWI76_RS24835 at 5175.537 kb on + strand, within BWI76_RS24835 at 5175.540 kb on - strand, within BWI76_RS24835 at 5175.540 kb on - strand, within BWI76_RS24835 at 5175.540 kb on - strand, within BWI76_RS24835 at 5175.540 kb on - strand, within BWI76_RS24835 at 5175.547 kb on + strand, within BWI76_RS24835 at 5175.552 kb on + strand, within BWI76_RS24835 at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.560 kb on + strand at 5175.560 kb on + strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on - strand at 5175.568 kb on - strand at 5175.576 kb on - strand at 5175.576 kb on - strand at 5175.576 kb on - strand at 5175.576 kb on - strand at 5175.576 kb on - strand at 5175.576 kb on - strand at 5175.578 kb on + strand at 5175.578 kb on + strand at 5175.586 kb on - strand at 5175.586 kb on - strand at 5175.587 kb on + strand at 5175.587 kb on + strand at 5175.595 kb on - strand at 5175.595 kb on - strand at 5175.652 kb on + strand at 5175.665 kb on + strand at 5175.704 kb on + strand at 5175.781 kb on + strand at 5175.797 kb on + strand at 5175.814 kb on - strand at 5175.816 kb on + strand at 5175.816 kb on + strand at 5175.824 kb on + strand at 5175.824 kb on - strand at 5175.824 kb on - strand at 5175.824 kb on - strand at 5175.824 kb on - strand at 5175.824 kb on - strand at 5175.826 kb on + strand at 5175.826 kb on + strand at 5175.826 kb on + strand at 5175.826 kb on + strand at 5175.827 kb on + strand at 5175.832 kb on - strand at 5175.832 kb on - strand at 5175.834 kb on - strand at 5175.834 kb on - strand at 5175.835 kb on - strand at 5175.835 kb on - strand at 5175.836 kb on + strand at 5175.886 kb on + strand at 5175.900 kb on + strand at 5175.938 kb on + strand at 5175.953 kb on - strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.958 kb on + strand at 5175.959 kb on + strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.982 kb on + strand at 5176.069 kb on + strand at 5176.077 kb on - strand at 5176.077 kb on - strand at 5176.127 kb on + strand at 5176.149 kb on - strand at 5176.160 kb on + strand at 5176.179 kb on - strand at 5176.179 kb on - strand at 5176.180 kb on - strand at 5176.194 kb on + strand at 5176.194 kb on + strand at 5176.194 kb on + strand at 5176.202 kb on - strand at 5176.203 kb on + strand at 5176.203 kb on + strand at 5176.203 kb on + strand at 5176.203 kb on + strand at 5176.203 kb on + strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.213 kb on + strand at 5176.213 kb on + strand at 5176.213 kb on + strand at 5176.213 kb on + strand at 5176.215 kb on - strand at 5176.221 kb on - strand at 5176.231 kb on + strand at 5176.292 kb on + strand, within BWI76_RS24840 at 5176.292 kb on + strand, within BWI76_RS24840 at 5176.292 kb on + strand, within BWI76_RS24840 at 5176.300 kb on - strand, within BWI76_RS24840 at 5176.300 kb on - strand, within BWI76_RS24840 at 5176.315 kb on - strand, within BWI76_RS24840 at 5176.316 kb on + strand, within BWI76_RS24840 at 5176.324 kb on - strand, within BWI76_RS24840 at 5176.327 kb on - strand, within BWI76_RS24840 at 5176.356 kb on - strand, within BWI76_RS24840 at 5176.427 kb on + strand, within BWI76_RS24840 at 5176.507 kb on - strand, within BWI76_RS24840 at 5176.669 kb on - strand, within BWI76_RS24840
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 5,173,533 - -1.3 5,173,636 - BWI76_RS24830 0.11 +1.7 5,173,639 + BWI76_RS24830 0.11 +2.7 5,173,741 + BWI76_RS24830 0.24 +0.0 5,173,777 - BWI76_RS24830 0.29 +0.6 5,173,864 - BWI76_RS24830 0.40 +1.6 5,173,923 + BWI76_RS24830 0.48 +1.7 5,173,923 + BWI76_RS24830 0.48 +1.1 5,174,191 - BWI76_RS24830 0.83 -1.3 5,174,326 + +0.9 5,174,334 - -0.8 5,174,412 + -0.1 5,174,446 + +1.2 5,174,487 - BWI76_RS24835 0.10 -0.1 5,174,586 + BWI76_RS24835 0.18 +0.8 5,174,796 - BWI76_RS24835 0.33 +3.6 5,174,814 + BWI76_RS24835 0.35 -0.6 5,174,952 - BWI76_RS24835 0.45 +0.6 5,175,082 - BWI76_RS24835 0.55 -1.8 5,175,103 - BWI76_RS24835 0.56 +0.3 5,175,116 + BWI76_RS24835 0.57 +2.2 5,175,116 + BWI76_RS24835 0.57 +1.6 5,175,208 + +1.2 5,175,223 + BWI76_RS24835 0.65 -0.8 5,175,234 + BWI76_RS24835 0.66 -1.1 5,175,330 - BWI76_RS24835 0.73 -0.2 5,175,353 + BWI76_RS24835 0.75 -3.1 5,175,469 + BWI76_RS24835 0.84 +0.9 5,175,506 - BWI76_RS24835 0.86 +1.0 5,175,506 - BWI76_RS24835 0.86 +0.3 5,175,522 + BWI76_RS24835 0.88 +0.4 5,175,530 - BWI76_RS24835 0.88 -0.8 5,175,530 - BWI76_RS24835 0.88 +1.8 5,175,537 + BWI76_RS24835 0.89 +1.4 5,175,540 - BWI76_RS24835 0.89 -0.8 5,175,540 - BWI76_RS24835 0.89 -2.6 5,175,540 - BWI76_RS24835 0.89 +0.6 5,175,540 - BWI76_RS24835 0.89 -1.7 5,175,547 + BWI76_RS24835 0.90 +0.5 5,175,552 + BWI76_RS24835 0.90 +0.5 5,175,557 + -2.2 5,175,557 + -2.2 5,175,557 + -0.8 5,175,557 + -2.3 5,175,557 + +0.8 5,175,557 + -2.3 5,175,557 + -0.7 5,175,557 + +0.4 5,175,557 + +0.8 5,175,557 + -2.7 5,175,557 + -1.1 5,175,557 + -1.1 5,175,557 + +2.4 5,175,557 + -3.2 5,175,557 + -3.5 5,175,557 + -1.6 5,175,557 + -1.5 5,175,560 + -1.9 5,175,560 + +1.9 5,175,565 - -0.2 5,175,565 - +0.9 5,175,565 - +0.2 5,175,565 - +1.7 5,175,565 - +0.6 5,175,565 - -1.9 5,175,565 - -0.2 5,175,565 - +0.2 5,175,565 - -2.5 5,175,565 - -2.6 5,175,565 - -0.6 5,175,565 - -0.5 5,175,565 - +0.2 5,175,565 - -3.1 5,175,565 - +0.8 5,175,565 - -0.6 5,175,568 + +1.5 5,175,568 + -3.5 5,175,568 + +0.2 5,175,568 + +1.4 5,175,568 + -0.2 5,175,568 + -1.2 5,175,568 + +0.2 5,175,568 + +0.8 5,175,568 + -1.9 5,175,568 + -0.1 5,175,568 - +2.4 5,175,568 - -1.9 5,175,576 - -0.8 5,175,576 - +1.2 5,175,576 - -1.0 5,175,576 - -4.0 5,175,576 - -3.0 5,175,576 - +0.9 5,175,578 + -2.5 5,175,578 + +1.0 5,175,586 - -0.3 5,175,586 - +1.2 5,175,587 + +0.2 5,175,587 + -1.1 5,175,595 - +2.0 5,175,595 - -1.3 5,175,652 + -0.7 5,175,665 + +1.7 5,175,704 + -1.8 5,175,781 + +1.2 5,175,797 + -0.8 5,175,814 - -3.4 5,175,816 + +2.5 5,175,816 + -2.1 5,175,824 + +0.2 5,175,824 - -2.0 5,175,824 - +0.9 5,175,824 - -2.3 5,175,824 - +0.5 5,175,824 - -2.3 5,175,826 + -0.3 5,175,826 + -0.8 5,175,826 + -0.8 5,175,826 + +0.2 5,175,827 + -0.8 5,175,832 - +0.9 5,175,832 - +3.6 5,175,834 - +1.2 5,175,834 - -0.8 5,175,835 - -1.1 5,175,835 - -0.6 5,175,836 + -1.9 5,175,886 + -1.8 5,175,900 + -4.3 5,175,938 + +0.8 5,175,953 - +0.1 5,175,957 + +1.3 5,175,957 + +1.7 5,175,957 + -0.3 5,175,957 + -0.8 5,175,957 + +0.2 5,175,957 + -1.3 5,175,957 + +1.2 5,175,958 + -3.5 5,175,959 + +0.7 5,175,965 - +0.7 5,175,965 - +1.2 5,175,965 - -0.8 5,175,965 - -1.0 5,175,965 - +2.2 5,175,965 - -2.8 5,175,965 - -4.3 5,175,965 - +1.0 5,175,982 + -3.2 5,176,069 + -0.1 5,176,077 - -4.1 5,176,077 - +1.2 5,176,127 + -3.2 5,176,149 - +1.7 5,176,160 + +0.2 5,176,179 - -4.7 5,176,179 - -0.3 5,176,180 - -1.8 5,176,194 + +0.2 5,176,194 + +1.3 5,176,194 + +0.4 5,176,202 - -1.1 5,176,203 + -1.2 5,176,203 + +0.2 5,176,203 + -0.1 5,176,203 + +0.1 5,176,203 + +0.5 5,176,211 - -1.3 5,176,211 - -1.3 5,176,211 - +1.0 5,176,211 - -2.1 5,176,211 - +1.2 5,176,211 - +0.3 5,176,211 - +1.3 5,176,211 - +0.8 5,176,213 + -0.3 5,176,213 + -0.8 5,176,213 + +0.3 5,176,213 + +0.7 5,176,215 - -3.8 5,176,221 - -4.0 5,176,231 + +2.1 5,176,292 + BWI76_RS24840 0.14 -2.9 5,176,292 + BWI76_RS24840 0.14 -0.4 5,176,292 + BWI76_RS24840 0.14 -1.1 5,176,300 - BWI76_RS24840 0.14 -0.3 5,176,300 - BWI76_RS24840 0.14 -4.3 5,176,315 - BWI76_RS24840 0.15 -1.0 5,176,316 + BWI76_RS24840 0.15 -0.0 5,176,324 - BWI76_RS24840 0.16 +0.2 5,176,327 - BWI76_RS24840 0.16 -1.1 5,176,356 - BWI76_RS24840 0.18 +0.2 5,176,427 + BWI76_RS24840 0.22 -2.8 5,176,507 - BWI76_RS24840 0.27 -0.5 5,176,669 - BWI76_RS24840 0.38 +1.1
Or see this region's nucleotide sequence