Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23345

Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23335 and BWI76_RS23340 overlap by 4 nucleotidesBWI76_RS23340 and BWI76_RS23345 are separated by 68 nucleotidesBWI76_RS23345 and BWI76_RS23350 are separated by 7 nucleotides BWI76_RS23335: BWI76_RS23335 - GNAT family acetyltransferase, at 4,865,817 to 4,866,296 _RS23335 BWI76_RS23340: BWI76_RS23340 - transcriptional regulator, at 4,866,293 to 4,867,066 _RS23340 BWI76_RS23345: BWI76_RS23345 - glycoside hydrolase, at 4,867,135 to 4,868,562 _RS23345 BWI76_RS23350: BWI76_RS23350 - PTS system lactose/cellobiose family transporter subunit IIC, at 4,868,570 to 4,869,907 _RS23350 Position (kb) 4867 4868 4869Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 4866.161 kb on + strand, within BWI76_RS23335at 4866.251 kb on + strandat 4866.274 kb on + strandat 4866.283 kb on + strandat 4866.296 kb on + strandat 4866.597 kb on + strand, within BWI76_RS23340at 4866.858 kb on - strand, within BWI76_RS23340at 4867.718 kb on + strand, within BWI76_RS23345at 4868.058 kb on + strand, within BWI76_RS23345at 4868.198 kb on - strand, within BWI76_RS23345at 4868.198 kb on - strand, within BWI76_RS23345at 4868.252 kb on - strand, within BWI76_RS23345at 4868.511 kb on + strandat 4868.906 kb on + strand, within BWI76_RS23350at 4868.914 kb on - strand, within BWI76_RS23350at 4868.966 kb on + strand, within BWI76_RS23350at 4869.070 kb on + strand, within BWI76_RS23350at 4869.078 kb on - strand, within BWI76_RS23350at 4869.126 kb on + strand, within BWI76_RS23350at 4869.167 kb on - strandat 4869.206 kb on + strand, within BWI76_RS23350at 4869.242 kb on + strand, within BWI76_RS23350at 4869.250 kb on - strand, within BWI76_RS23350at 4869.262 kb on + strand, within BWI76_RS23350

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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4,866,161 + BWI76_RS23335 0.72 +0.2
4,866,251 + +0.6
4,866,274 + -2.2
4,866,283 + -3.4
4,866,296 + +1.5
4,866,597 + BWI76_RS23340 0.39 -0.3
4,866,858 - BWI76_RS23340 0.73 -1.0
4,867,718 + BWI76_RS23345 0.41 +1.0
4,868,058 + BWI76_RS23345 0.65 +1.8
4,868,198 - BWI76_RS23345 0.74 -3.6
4,868,198 - BWI76_RS23345 0.74 +0.3
4,868,252 - BWI76_RS23345 0.78 +1.4
4,868,511 + -0.4
4,868,906 + BWI76_RS23350 0.25 +0.2
4,868,914 - BWI76_RS23350 0.26 -2.7
4,868,966 + BWI76_RS23350 0.30 -0.1
4,869,070 + BWI76_RS23350 0.37 -0.6
4,869,078 - BWI76_RS23350 0.38 +4.0
4,869,126 + BWI76_RS23350 0.42 +0.7
4,869,167 - -0.4
4,869,206 + BWI76_RS23350 0.48 +1.0
4,869,242 + BWI76_RS23350 0.50 +1.0
4,869,250 - BWI76_RS23350 0.51 -0.5
4,869,262 + BWI76_RS23350 0.52 -2.3

Or see this region's nucleotide sequence