Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19655 and BWI76_RS19660 are separated by 15 nucleotides BWI76_RS19660 and BWI76_RS19665 are separated by 242 nucleotides BWI76_RS19665 and BWI76_RS19670 overlap by 73 nucleotides BWI76_RS19670 and BWI76_RS19675 are separated by 53 nucleotides
BWI76_RS19655: BWI76_RS19655 - hypothetical protein, at 4,108,074 to 4,109,231
_RS19655
BWI76_RS19660: BWI76_RS19660 - GTP cyclohydrolase I FolE, at 4,109,247 to 4,109,915
_RS19660
BWI76_RS19665: BWI76_RS19665 - hypothetical protein, at 4,110,158 to 4,110,355
_RS19665
BWI76_RS19670: BWI76_RS19670 - S-formylglutathione hydrolase, at 4,110,283 to 4,111,119
_RS19670
BWI76_RS19675: BWI76_RS19675 - hypothetical protein, at 4,111,173 to 4,111,370
_RS19675
Position (kb) 4110
4111 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4109.236 kb on - strand at 4110.289 kb on - strand at 4110.568 kb on - strand, within BWI76_RS19670 at 4110.738 kb on + strand, within BWI76_RS19670 at 4111.299 kb on - strand, within BWI76_RS19675
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,109,236 - +0.5 4,110,289 - -3.5 4,110,568 - BWI76_RS19670 0.34 -1.1 4,110,738 + BWI76_RS19670 0.54 -3.6 4,111,299 - BWI76_RS19675 0.64 +1.2
Or see this region's nucleotide sequence