Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15335 and BWI76_RS15340 are separated by 84 nucleotides BWI76_RS15340 and BWI76_RS15345 are separated by 176 nucleotides
BWI76_RS15335: BWI76_RS15335 - branched-chain amino acid ABC transporter permease, at 3,210,917 to 3,212,491
_RS15335
BWI76_RS15340: BWI76_RS15340 - urea ABC transporter substrate-binding protein, at 3,212,576 to 3,213,847
_RS15340
BWI76_RS15345: BWI76_RS15345 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 3,214,024 to 3,215,127
_RS15345
Position (kb)
3212
3213
3214 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3211.850 kb on + strand, within BWI76_RS15335 at 3211.885 kb on - strand, within BWI76_RS15335 at 3212.101 kb on + strand, within BWI76_RS15335 at 3212.534 kb on + strand at 3212.758 kb on + strand, within BWI76_RS15340 at 3213.004 kb on - strand, within BWI76_RS15340 at 3213.110 kb on - strand, within BWI76_RS15340 at 3213.476 kb on - strand, within BWI76_RS15340 at 3213.488 kb on - strand, within BWI76_RS15340 at 3213.794 kb on + strand at 3213.795 kb on - strand at 3213.797 kb on + strand at 3213.856 kb on + strand at 3213.856 kb on + strand at 3213.856 kb on + strand at 3213.864 kb on - strand at 3213.864 kb on - strand at 3213.957 kb on - strand at 3214.012 kb on + strand at 3214.033 kb on - strand at 3214.040 kb on - strand at 3214.159 kb on - strand, within BWI76_RS15345 at 3214.451 kb on + strand, within BWI76_RS15345 at 3214.489 kb on + strand, within BWI76_RS15345 at 3214.554 kb on - strand, within BWI76_RS15345 at 3214.844 kb on - strand, within BWI76_RS15345
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,211,850 + BWI76_RS15335 0.59 +0.8 3,211,885 - BWI76_RS15335 0.61 -0.3 3,212,101 + BWI76_RS15335 0.75 +0.4 3,212,534 + -2.4 3,212,758 + BWI76_RS15340 0.14 +1.3 3,213,004 - BWI76_RS15340 0.34 -0.3 3,213,110 - BWI76_RS15340 0.42 -3.1 3,213,476 - BWI76_RS15340 0.71 -1.3 3,213,488 - BWI76_RS15340 0.72 +0.8 3,213,794 + +0.1 3,213,795 - -0.4 3,213,797 + +0.1 3,213,856 + +1.1 3,213,856 + +2.4 3,213,856 + +1.0 3,213,864 - +1.3 3,213,864 - +0.3 3,213,957 - +0.5 3,214,012 + -0.2 3,214,033 - -2.4 3,214,040 - -1.2 3,214,159 - BWI76_RS15345 0.12 +1.9 3,214,451 + BWI76_RS15345 0.39 +1.4 3,214,489 + BWI76_RS15345 0.42 -0.1 3,214,554 - BWI76_RS15345 0.48 +1.1 3,214,844 - BWI76_RS15345 0.74 +0.1
Or see this region's nucleotide sequence