Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14360

Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
3,000,376	+				-1.8
3,000,445	-		BWI76_RS14350	0.17	-3.0
3,000,494	+		BWI76_RS14350	0.41	+0.5
3,000,503	+		BWI76_RS14350	0.46	+3.1
3,000,536	+		BWI76_RS14350	0.62	+0.4
3,000,544	-		BWI76_RS14350	0.66	+1.7
3,000,544	-		BWI76_RS14350	0.66	+0.9
3,000,544	-		BWI76_RS14350	0.66	-0.6
3,000,544	-		BWI76_RS14350	0.66	+1.2
3,000,580	-		BWI76_RS14350	0.84	-2.5
3,000,660	-				-1.7
3,000,722	-				+1.1
3,000,758	+				-3.3
3,000,813	+				+1.4
3,000,821	-				+0.4
3,000,844	+				-0.2
3,000,844	+				+0.3
3,000,844	+				-1.3
3,000,844	+				+2.1
3,000,852	-				-3.4
3,000,852	-				+0.5
3,000,867	-				+1.4
3,000,881	-				+0.1
3,000,887	+				+0.1
3,000,895	-				-1.5
3,000,926	+		BWI76_RS14355	0.27	+0.7
3,000,953	+		BWI76_RS14355	0.63	-2.5
3,001,015	-				+1.1
3,001,048	+				+0.9
3,001,070	+				-4.4
3,001,093	+				+0.1
3,001,095	+				-1.3
3,001,095	+				-0.4
3,001,096	+				-0.1
3,001,104	-				-1.5
3,001,106	+				+1.1
3,001,106	+				+1.3
3,001,106	+				-0.5
3,001,106	+				-3.1
3,001,114	-				-1.9
3,001,114	-				-3.1
3,001,123	+				-0.2
3,001,129	+				-1.7
3,001,135	+				+0.8
3,001,142	+				+0.4
3,001,142	+				-1.0
3,001,145	-				-1.5
3,001,183	+				+4.0
3,001,183	+				-0.8
3,001,183	+				-0.5
3,001,183	+				+0.1
3,001,245	+				-0.3
3,001,245	+				-0.5
3,001,245	+				+1.1
3,001,245	+				+0.7
3,001,245	+				-3.2
3,001,245	+				+0.7
3,001,245	+				+0.6
3,001,245	+				+0.6
3,001,245	+				+0.7
3,001,245	+				-0.6
3,001,245	+				+0.3
3,001,245	+				+0.1
3,001,247	+				-0.1
3,001,247	+				-2.4
3,001,252	+				+1.5
3,001,253	-				+0.9
3,001,253	-				+1.1
3,001,255	-				-0.2
3,001,282	+				+0.2
3,001,284	+				+0.4
3,001,284	+				+0.3
3,001,295	+				+0.2
3,001,312	+				+0.1
3,001,320	-				-2.3
3,001,344	+				-1.7
3,001,463	-		BWI76_RS14360	0.16	-0.9
3,001,468	-		BWI76_RS14360	0.17	+3.3
3,001,500	-		BWI76_RS14360	0.23	-0.5
3,001,905	+				+4.9
3,001,913	-				-0.5
3,001,976	-				+2.1
3,001,976	-				+0.5
3,002,083	+		BWI76_RS14365	0.16	-0.0
3,002,094	+		BWI76_RS14365	0.20	-2.3
3,002,209	+		BWI76_RS14365	0.54	-0.2
3,002,209	+		BWI76_RS14365	0.54	-2.0
3,002,245	+		BWI76_RS14365	0.65	+0.3
3,002,250	+		BWI76_RS14365	0.66	-0.5
3,002,250	+		BWI76_RS14365	0.66	-1.6
3,002,255	+		BWI76_RS14365	0.68	+0.2
3,002,261	+		BWI76_RS14365	0.70	+0.5
3,002,290	+		BWI76_RS14365	0.78	-1.5
3,002,306	+		BWI76_RS14365	0.83	+1.1
3,002,408	+		BWI76_RS14370	0.32	-2.5
3,002,408	+		BWI76_RS14370	0.32	+0.0
3,002,408	+		BWI76_RS14370	0.32	-4.2
3,002,408	+		BWI76_RS14370	0.32	+0.0
3,002,408	+		BWI76_RS14370	0.32	-1.5
3,002,408	+		BWI76_RS14370	0.32	+0.3
3,002,408	+		BWI76_RS14370	0.32	+0.8
3,002,408	+		BWI76_RS14370	0.32	-2.5
3,002,408	+		BWI76_RS14370	0.32	+0.1
3,002,408	+		BWI76_RS14370	0.32	+1.1
3,002,408	+		BWI76_RS14370	0.32	+0.0
3,002,408	+		BWI76_RS14370	0.32	+0.1
3,002,408	+		BWI76_RS14370	0.32	+0.2
3,002,416	-		BWI76_RS14370	0.36	+1.5
3,002,431	+		BWI76_RS14370	0.44	+0.6
3,002,431	+		BWI76_RS14370	0.44	-1.0
3,002,436	+		BWI76_RS14370	0.47	+1.0
3,002,436	+		BWI76_RS14370	0.47	-0.2
3,002,436	+		BWI76_RS14370	0.47	-1.4
3,002,441	+		BWI76_RS14370	0.49	-3.8
3,002,462	+		BWI76_RS14370	0.60	-2.8
3,002,462	+		BWI76_RS14370	0.60	-2.7
3,002,470	-				-0.5
3,002,472	+		BWI76_RS14370	0.65	-1.3
3,002,472	+		BWI76_RS14370	0.65	-1.2
3,002,472	+		BWI76_RS14370	0.65	+1.1
3,002,472	+		BWI76_RS14370	0.65	+0.4
3,002,477	+		BWI76_RS14370	0.68	-0.3
3,002,480	-		BWI76_RS14370	0.69	+1.9
3,002,594	+				+0.1
3,002,602	-				+0.5
3,002,602	-				-2.1
3,002,762	-		BWI76_RS14375	0.24	-0.1
3,002,801	+		BWI76_RS14375	0.33	-1.9
3,002,801	+		BWI76_RS14375	0.33	-2.7
3,002,838	-		BWI76_RS14375	0.42	-2.1
3,002,901	+		BWI76_RS14375	0.56	-2.5