Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14025 and BWI76_RS14030 are separated by 24 nucleotides BWI76_RS14030 and BWI76_RS14035 overlap by 4 nucleotides BWI76_RS14035 and BWI76_RS14040 overlap by 4 nucleotides
BWI76_RS14025: BWI76_RS14025 - NMT1/THI5 like domain-containing protein, at 2,931,540 to 2,932,475
_RS14025
BWI76_RS14030: BWI76_RS14030 - ABC transporter permease, at 2,932,500 to 2,933,240
_RS14030
BWI76_RS14035: BWI76_RS14035 - ABC transporter ATPase, at 2,933,237 to 2,933,965
_RS14035
BWI76_RS14040: BWI76_RS14040 - thiaminase, at 2,933,962 to 2,934,657
_RS14040
Position (kb)
2932
2933
2934 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2931.533 kb on + strand at 2931.538 kb on + strand at 2931.546 kb on - strand at 2931.546 kb on - strand at 2931.546 kb on - strand at 2931.546 kb on - strand at 2931.588 kb on - strand at 2931.589 kb on + strand at 2931.600 kb on + strand at 2931.601 kb on + strand at 2931.619 kb on - strand at 2931.620 kb on - strand at 2931.639 kb on - strand, within BWI76_RS14025 at 2931.665 kb on + strand, within BWI76_RS14025 at 2931.672 kb on + strand, within BWI76_RS14025 at 2931.703 kb on + strand, within BWI76_RS14025 at 2931.703 kb on + strand, within BWI76_RS14025 at 2931.711 kb on - strand, within BWI76_RS14025 at 2931.734 kb on + strand, within BWI76_RS14025 at 2931.772 kb on - strand, within BWI76_RS14025 at 2931.898 kb on + strand, within BWI76_RS14025 at 2932.013 kb on - strand, within BWI76_RS14025 at 2932.197 kb on + strand, within BWI76_RS14025 at 2932.483 kb on - strand at 2932.527 kb on + strand at 2932.563 kb on + strand at 2932.789 kb on - strand, within BWI76_RS14030 at 2932.819 kb on + strand, within BWI76_RS14030 at 2933.013 kb on - strand, within BWI76_RS14030 at 2933.034 kb on - strand, within BWI76_RS14030 at 2933.228 kb on + strand at 2933.236 kb on - strand at 2933.485 kb on - strand, within BWI76_RS14035 at 2933.485 kb on - strand, within BWI76_RS14035 at 2933.616 kb on - strand, within BWI76_RS14035 at 2933.701 kb on + strand, within BWI76_RS14035 at 2933.701 kb on + strand, within BWI76_RS14035 at 2933.937 kb on - strand at 2933.994 kb on - strand at 2934.013 kb on - strand at 2934.039 kb on - strand, within BWI76_RS14040 at 2934.039 kb on - strand, within BWI76_RS14040
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,931,533 + +2.8 2,931,538 + +0.3 2,931,546 - +0.9 2,931,546 - -3.2 2,931,546 - -0.1 2,931,546 - -0.7 2,931,588 - +0.1 2,931,589 + +0.8 2,931,600 + -1.1 2,931,601 + +0.2 2,931,619 - +1.4 2,931,620 - +0.5 2,931,639 - BWI76_RS14025 0.11 +0.6 2,931,665 + BWI76_RS14025 0.13 +1.3 2,931,672 + BWI76_RS14025 0.14 -1.3 2,931,703 + BWI76_RS14025 0.17 +0.1 2,931,703 + BWI76_RS14025 0.17 -1.5 2,931,711 - BWI76_RS14025 0.18 +0.1 2,931,734 + BWI76_RS14025 0.21 +0.1 2,931,772 - BWI76_RS14025 0.25 -0.1 2,931,898 + BWI76_RS14025 0.38 -2.2 2,932,013 - BWI76_RS14025 0.51 +0.4 2,932,197 + BWI76_RS14025 0.70 +0.6 2,932,483 - +1.0 2,932,527 + +0.8 2,932,563 + +0.7 2,932,789 - BWI76_RS14030 0.39 +1.5 2,932,819 + BWI76_RS14030 0.43 -0.0 2,933,013 - BWI76_RS14030 0.69 +1.6 2,933,034 - BWI76_RS14030 0.72 +0.7 2,933,228 + -1.5 2,933,236 - +1.0 2,933,485 - BWI76_RS14035 0.34 +0.5 2,933,485 - BWI76_RS14035 0.34 +0.5 2,933,616 - BWI76_RS14035 0.52 +0.4 2,933,701 + BWI76_RS14035 0.64 +0.5 2,933,701 + BWI76_RS14035 0.64 -0.5 2,933,937 - +1.1 2,933,994 - +1.2 2,934,013 - -1.0 2,934,039 - BWI76_RS14040 0.11 -1.8 2,934,039 - BWI76_RS14040 0.11 +0.6
Or see this region's nucleotide sequence